In:
Japanese Journal of Applied Physics, IOP Publishing, Vol. 43, No. 7S ( 2004-07-01), p. 4599-
Abstract:
Scanning force microscopy (SFM) was used to image ribosomes and ribosomal subunits isolated from Escherichia coli . Topographic images of ribosomal particles adsorbed on a mica surface were obtained directly. At 80 nM, the ribosomes aggregated. At ≤8 nM, a sparser coverage was achieved, with single ribosomes isolated from one another. Although the obtained height was significantly lower than the diameter measured by X-ray diffraction, the height histogram revealed that the observed ribosomal particles belong to three height distributions: approximately 4, 9 and 11 nm. These three distributions correspond to the 70S ribosome, and the 50S and 30S ribosomal subunits, respectively. This work demonstrates that SFM is useful in the discrimination of very small amounts of ribosomal particles on a solid substrate surface.
Type of Medium:
Online Resource
ISSN:
0021-4922
,
1347-4065
DOI:
10.1143/JJAP.43.4599
Language:
Unknown
Publisher:
IOP Publishing
Publication Date:
2004
detail.hit.zdb_id:
218223-3
detail.hit.zdb_id:
797294-5
detail.hit.zdb_id:
2006801-3
detail.hit.zdb_id:
797295-7
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