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  • 1
    In: Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
    Abstract: Introduction: Currently, Vietnam is entering the aging population stage and is one of the fastest aging countries worldwide. Old age increases the risk of chronic diseases, particularly mental health disorders. Objective: investigated the prevalence of depression and examined the associated factors with depression among older people. Methods: A cross-sectional study was implemented in 760 older people in some communities of Thua Thien Hue province from January to August 2020. Data were collected by directinterviewing based on a structured questionnaire, including demographic, socio-economic, geriatric Depression scale with 30 questions (GDS-30), health status and physical activities. The GDS-30 was used to evaluate the prevalence of depression. The multiple logistic regression model was applied to exam the associated factors with depression. Results: Our findings indicated that the prevalence of depression among elderly people was 28.6%, comprising mild (23.6%) and seveve (5%). The higher Odds of depression was observed in the low economic status (poor/near-poor) (OR= 2.51; 95% CI: 1.15 – 5.48), live alone (OR= 2.43; 95% CI: 1.02-5.78), co-morbidities chronic disease(OR =1,59; 95% CI: 1.01 – 2.52), self-evaluation not good in overall health status (OR =2.34; 95% CI: 1,50 – 3,66), dissatisfaction in health (OR = 2.55; 95% CI:1.59 – 4.08), lack of physical activities (OR =2.79; 95% CI: 1.83 - 4.27), and low quality of life (OR = 2.79; 95% CI: 1.84-4.24). Conclusions: This research suggests that older people are commonly exposing to depression in the communities. Therefore, the priority strategies for reducing depression should be implemented, particularly in the high-risk groups.
    Type of Medium: Online Resource
    ISSN: 1859-3836
    Language: Vietnamese
    Publisher: Hue University of Medicine and Pharmacy
    Publication Date: 2021
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  • 2
    In: Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
    Abstract: Background: To evaluate the complete blood count of Laotian students in Hue city. Materials and method: A cross-sectional study on 400 Lao students who were examinated the complete blood count at Department of Hematology, Hue University of Medicine and Pharmacy from September 2019 to May 2020. Results: 400 Lao students (male/female = 1/1.2), the students who came from Salavan province accounted for the highest percentage (22.25%). The mean of age was 24.78 ± 6.44 (17 - 44). The mean of red blood cells (RBC) was 5.13 ± 0.73 T/L and of hemoglobin in the study group was 129.27 ± 18.58 g/L. The percentage of students with anemia was 13.5% and there were 3 students with severe anemia. The mean of mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were respectively 81.27 ± 9.32 fL (49.2 - 103.9) and 25.50 ± 3.56 pg (11.8 - 33.2). The mean of white blood cells (WBC) and platelets (PLT) were respectively 7.42 ± 2.29 G/L and 272.33 ± 83.62 G/L. There was a correlation between anemia and age (p 〈 0.05), mainly in 26 - 40. There was significant difference about RBC and Hb between anemic group and non-anemic group (p 〈 0.05). Conclusion: It is essential to check the complete blood count of Laotian students to evaluate anemia and screen hemoglobinopathies. Keywords: Laotian students, red blood cell (RBC), white blood cell (WBC), platelet (PLT), hemoglobin (Hb)
    Type of Medium: Online Resource
    ISSN: 1859-3836
    Language: Vietnamese
    Publisher: Hue University of Medicine and Pharmacy
    Publication Date: 2020
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  • 3
    In: Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
    Abstract: Background: Drug-resistant Tuberculosis (DR-TB) is challenging public health problem in countries with high tuberculosis ​​prevalence and limited resources. Developing and applying the most appropriate and effective methods for diagnosing DR-TB from clinical samples is necessary, allowing a more rapid detection method for large-scale screening. Methods: Applying real-time PCR Taqman allelic discrimination with a PCR Taqman probes panel to identifying the DR-TB associated mutations in rpoB and katG of Mycobacterium Tuberculosis from isolates and clinical samples. Results: Comparing results of the real-time PCR allelic and DNA sequencing results, the sensitivity and specificity for Isoniazid resistance detection by analysing katG were found 95%(75.1 - 99.8) and 100%, Rifampicin resistance determining region (RRDR) of rpoB were found 95.5(77.16 - 99.88) and 100%, respectively. The real-time PCR TaqMan allelic discrimination also showed the sensitivities 100% for both katG and rpoB, and the specificities were 93.55% (78.58 - 99.21) for the rpoB and 93.94% (79.77 - 99.26) for the katG from clinical samples. Conclusions: This study showed that the real-time PCR taqman allelic discrimination assay is useful for detection of TB and DR-TB because of an accurate and rapid diagnosis in the early stages. Key words: rug-resistant, Tuberculosis, clinical samples, real-time PCR taqman allelic discrimination assay, Mycobacterium tuberculosis.
    Type of Medium: Online Resource
    ISSN: 1859-3836
    Language: Vietnamese
    Publisher: Hue University of Medicine and Pharmacy
    Publication Date: 2023
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  • 4
    In: Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
    Abstract: Introduction: Bacterial meningitis is an acute central nervous infection with high mortality or permanent neurological sequelae if remained undiagnosed. However, traditional diagnostic methods for bacterial meningitis pose challenge in prompt and precise identification of causative agents. Aims: The present study will therefore aim to set up in-house PCR assays for diagnosis of six pathogens causing the disease including H. influenzae type b, S. pneumoniae, N. meningitidis, S. suis serotype 2, E. coli and S. aureus. Methods: inhouse PCR assays for detecting six above-mentioned bacteria were optimized after specific pairs of primers and probes collected from the reliable literature resources and then were performed for cerebrospinal fluid (CSF) samples from patients with suspected meningitis in Hue Hospitals. Results: The set of four PCR assays was developed including a multiplex real-time PCR for S. suis serotype 2, H. influenzae type b and N. meningitides; three monoplex real-time PCRs for E. coli, S. aureus and S. pneumoniae. Application of the in-house PCRs for 116 CSF samples, the results indicated that 48 (39.7%) cases were positive with S. suis serotype 2; one case was positive with H. influenzae type b; 4 cases were positive with E. coli; pneumococcal meningitis were 19 (16.4%) cases, meningitis with S. aureus and N. meningitidis were not observed in any CSF samples in this study. Conclusion: our in-house real-time PCR assays are rapid, sensitive and specific tools for routine diagnosis to detect six mentioned above meningitis etiological agents. Key words: Bacterial meningitis, etiological agents, multiplex real-time PCR
    Type of Medium: Online Resource
    ISSN: 1859-3836
    Language: Vietnamese
    Publisher: Hue University of Medicine and Pharmacy
    Publication Date: 2019
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  • 5
    In: Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
    Abstract: Introduction: Human Papilloma Virus (HPV) is a common sexually transmitted virus. HPV causes many diseases and the risk for many types of cancer which has been reported in both sexes, not just cervical cancer in females. However, the prevention of HPV infection is quite limited among males in Vietnam. The study is to evaluate knowledge, attitude on prevention of HPV infection among parents of male students at some junior high schools in Hue city and explore some related factors. Methods: A cross-sectional study was conducted among 785 parents of male students at four junior high schools in Hue city. Data were collected by direct interviews with structured questionnaire. Multivariate logistic regression model was used to identify the related factors. Results: Participants who got good knowledge and positive attitude of HPV infection prevention accounted for 18.9% and 15.2%, respectively. Occupation, educational level of parents, number of sons and family history of HPV related diseases influenced on knowledge about HPV infection prevention. Relations with son, educational level of parents, defect/chronic disease of son and knowledge of parents were significantly associated with their attitude toward HPV infection prevention (p 〈 0.05). Conclusions: This study highlighted that knowledge, attitude on HPV infection prevention of the parents of male students in Hue city was still low. The health sector needs to implement comprehensive health education and communication programs to improve knowledge, attitude about prevention of HPV infection for people, especially males. Key words: knowledge, attitude, human papilloma virus, HPV, parents, male students
    Type of Medium: Online Resource
    ISSN: 1859-3836
    Language: Vietnamese
    Publisher: Hue University of Medicine and Pharmacy
    Publication Date: 2022
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  • 6
    In: Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
    Abstract: Introduction: Infection with HPV is the main cause of cervical cancer. Determining HPV infection and the types of HPV plays an important role in diagnosis, treatment and prognosis of cervicitis/cervical cancer. Aims: Determining proportion of high-risk HPV types and the occurrence of coinfection with multiple HPV types. Methods: 177 women with cervicitis or abnormal Pap smear result were enrolled in the study. Performing the real-time PCR for detecting HPV and the reverse DOT-BLOT assay for determining type of HPV in cases of positive PCR. Results: 7 types of high-risk HPV was dectected, the majority of these types were HPV type 18 (74.6%) and HPV type 16 (37.6%); the proportion of infection with only one type of HPV was 30.4% and coinfection with multiple HPV types was higher (69.6%), the coinfected cases with 2 and 3 types were dominated (32.2% and 20.3%, respectively) and the coinfected cases with 4 and 5 types were rare. Conclusion: Use of the real-time PCR and reverse DOT-BLOT assay can determine the high-risk HPV types and the occurrence of coinfection with multiple HPV types. Key words: HPV type, Reverse DOT-BLOT, real-time PCR,PCR, cervical cancer
    Type of Medium: Online Resource
    ISSN: 1859-3836
    Language: Vietnamese
    Publisher: Hue University of Medicine and Pharmacy
    Publication Date: 2017
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  • 7
    In: Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
    Abstract: Background: The Nested IS6110 PCR is used for detecting tuberculosis, however IS6110 sequence is not present in the genome of all strains of M.tuberculosis, the result may be false negative. The gene coding 16S ribosome always contains a short sequence specific to M. tuberculosis complex. Objects: Performance of the 16S Real-time PCR to detect M. tuberculosis and combining to the nested IS6110 PCR to determine the rate of Mtb strains without IS6110 from clinical samples. Materials and method: Performance of 16S rDNA PCR by commercial kit of Viet A Inc. for all 480 samples, the samples which were positive with the 16S rDNA PCR were retested in IS6110 PCR assay by in-house kit. Results: The Realtime 16S rDNA PCR detected 258 cases (53.8%) of tuberculosis. There were 3 (1.2 %) M. tuberculosis strains which do not harbor IS6110 sequence in genome. Conclusion: The IS6110 nested PCR can be applied more widely than the 16S rDNA realtime PCR. In case of using IS6110 PCR assay, results may show a low proportion of false negative. Combining 16S rDNA PCR with the IS6110 based PCR allowed detection of deletion of IS6110 sequence in M. tuberculosis isolates.
    Type of Medium: Online Resource
    ISSN: 1859-3836
    Language: Vietnamese
    Publisher: Hue University of Medicine and Pharmacy
    Publication Date: 2012
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  • 8
    In: Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
    Abstract: Backround: Tuberculosis remains a major cause of morbidity and mortality in many countries and a significant public health problem worldwide. If TB is detected early and properly treated, the patients quickly become non-infectious and are eventually cured. Objective: Using MODS assay for detecting rapidly drug resistant and multidrug resistant M. tuberculosis. Methods: The MODS assay for the detection of tuberculosis and drug-resistant tuberculosis, directly from 252 sputum samples from suspected tuberculosis patients or untreated or ≤ 2 weeks treated tuberculosis patients. Culturing and doing susceptibility test by MODS assay (Issoniazid: 0.1 µg/ml; rifampicin: 1 µg/ml, Streptomycin: 2 µg/ml và Ethambutol: 2.5 µg/ml). Realtime PCR 16S was performed for the MODS culture-positive samples before 7 days and the samples with MODS culture-negative but AFB-positive. Results: M.tuberculosis was detected in 153 samples (60.7%) and 46 (30.1%) were antibiotic resistant. One drug resistance was present in 30 strains (19.6%): 18 for RIF, 6 for INH and 3 for STR and EBM. Multidrug resistant M.tuberculosis as defined by WHO (resistant to RIF and INH) was observed in 13 strains. There were additional 14 strains showing resistance to two or more drugs. Conclusion: The MODS assay is a rapid, direct method for simultaneous culture detection and drug susceptibility of M. tuberculosis, can be used as a routine procedure.
    Type of Medium: Online Resource
    ISSN: 1859-3836
    Language: Vietnamese
    Publisher: Hue University of Medicine and Pharmacy
    Publication Date: 2012
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  • 9
    Online Resource
    Online Resource
    Hue University of Medicine and Pharmacy ; 2014
    In:  Journal of Medicine and Pharmacy
    In: Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
    Abstract: Study objectives: To build a database recording all types of spoligotyping genes to serve the purpose of analyzing the result of spoligotyping technique in the study of the genetic diversity of Mycobacteria tuberculosis complex. Object and Methods: To use SpolDB4 as the reference database from Guadeloupe Paster Institute to employ PHP language for programming and record the spoligotyping data in MySQL. The module is run on the web platform. To build statistical functions according to different data entries and different searching tools according to various data input. Results: To complete the building of “SpoligoDB ver4” database to meet the demand of fast, visual and exact searching requirements and to fulfill the result testing process on spoligotyping technique among researches on classifications and drug-resistance and of Mycobacterium tuberculosis complex at Carlo Urbani Center, Hue University of Medicine and Pharmacy and other agencies at the following address http://khdn-yhue.vn/modules.php?name=Spoligodb; http://carlo-urbani-center.org/en/modules.php?name=Spoligodb. Conclusion: To complete the building of SpologoDB ver4 database from enucleated SpolDB4 database provided by the Guadeloupe Pasteur Institute and meet its requirements to serve the result analysis process on spoligotyping technique for those units that wish to research on the genetic diversity of Mycobacterium tuberculosis complex. Keywords: spoligotyping, spoligotype pattern, SpoligoDB ver4, AIE-VNM.
    Type of Medium: Online Resource
    ISSN: 1859-3836
    Language: Vietnamese
    Publisher: Hue University of Medicine and Pharmacy
    Publication Date: 2014
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  • 10
    Online Resource
    Online Resource
    Hue University of Medicine and Pharmacy ; 2023
    In:  Journal of Medicine and Pharmacy
    In: Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
    Abstract: Background: To achieve success in endodontic treatment, the working length must be precisely defined and maintained during preparation. The introduction of the electronic apex locator makes it possible to determine the working length quickly and accurately. Apex ID is the 4th generation electronic apex locator, which can determine the working length in humid conditions. The objective of the study was to evaluate the accuracy of the Apex ID apex locator. Materials and methods: In vitro study on 50 orthodontic extracted mandibular premolars, measured working length by Apex ID apex locator. The measured value was compared with the actual length of the canal and the working length was determined by periapical X-ray. The accuracy of Apex ID was assessed through the distribution of measured values within an acceptable deviation of 0.5mm from the apical foramen. Result: The working length measured by the Apex ID machine was shorter than that measured by the periapical X-ray and the actual length of the canal (p 〈 0.05). Within the accuracy of ±0.5mm compared to the apical foramen, the working length measured by the apex locator had an accuracy of 90%, higher than that measured by the X-ray at the rate of 80% (p 〈 0.05). Conclusion: Apex ID apex locator has high accuracy, and can replace X-ray film in determining working length in clinical practice. Key words: working length, electronic apex locator, Apex ID, in vitro
    Type of Medium: Online Resource
    ISSN: 1859-3836
    Language: Vietnamese
    Publisher: Hue University of Medicine and Pharmacy
    Publication Date: 2023
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