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  • Hindawi Limited  (3)
  • 1
    In: BioMed Research International, Hindawi Limited, Vol. 2017 ( 2017), p. 1-9
    Abstract: With the emerging role of umbilical cord blood-derived mesenchymal stem cells (hUCB-MSC) for bone regeneration and delivery of therapeutic proteins, there is an increasing need for effective gene delivery systems to modify such cells. mTAT, a TAT peptide sequence bearing histidine and cysteine residues, has been successfully used for intracellular gene delivery. Using a gWiz-GFP plasmid, we demonstrated that polyethylenimine combined with mTAT (mTAT/PEI) displayed good transfection efficacy in hUCB-MSC. hUCB-MSC transfected with mTAT/PEI were shown to express more BMP-2 protein and mRNA, indicating the feasibility of using the cells as a BMP-2 delivery system. Importantly, compared to PEI25, a “gold standard” nonviral transfection polymer, mTAT/PEI had limited toxicity to the cells. Furthermore, we demonstrated enhanced osteogenic activity in vitro for BMP-2 expressing hUCB-MSC. These results provide encouraging evidence for the potential use of mTAT/PEI to genetically modify hUCB-MSC as an approach to enhance tissue regeneration.
    Type of Medium: Online Resource
    ISSN: 2314-6133 , 2314-6141
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2017
    detail.hit.zdb_id: 2698540-8
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  • 2
    In: Geofluids, Hindawi Limited, Vol. 2021 ( 2021-7-30), p. 1-9
    Abstract: For the sake of studying the catastrophe mechanism of stress-fissure coupling field in mining close distance seams in southwest China, a test working face in Guizhou province in southwest China is adopted and researched by the methods of numerical calculation and similar experiment. When the working face advances to 180 m in 4# coal seam in a similar experiment, the overlying rock breaks to the central base plate of Yulongshan limestone, and the conductive fractures run through the Changxing limestone karst cave and Yulongshan limestone karst cave. When the 1402 working face advances to 350 m, the top of vertical karst caves in the middle of the model produces extrusion damage, forming a penetrating water inrush passage. When the 1402 working face advances to 480 m, the top slab of the working face comes under periodic weighting with the short step. Besides, the mining of 9# coal seam starts after 4# coal seam in mining close distance seams. When the working face in 9# coal seam advances to 340 m in numerical simulation, the maximum opening of the overburden fractures is 51.16 mm. The fractures in the roof are mainly caused by the periodic breaking and falling of the basic roof, connected with the floor fractures of 4# coal seam. When the working face in 9# coal seam advances to 500 m, the maximum opening of the overburden fracture is 93.09 mm. Specifically, as the working face advances, the opening of fracture in the roof after collapse of the basic roof periodically is mainly greater than 5 mm, and the compaction closure is mainly 1 mm-5 mm. The fractures in the gob floor are mainly 0.1 mm-1 mm, and the fracture opening of the collapsed rock mass in the gob is mainly 1 mm-5 mm and greater than 5 mm. The karst caves in the overburden reduce the periodic weighting step of working face and play a guiding role in the direction of fracture development and water inflow passage formation. The karst caves are connected to surface waterfall holes and trap pits, and atmospheric precipitation recharges the water in the caves. The research results can be treated as an important basis for the prevention and treatment for water inrush disaster in mining close distance seams in the karst area of southwest China.
    Type of Medium: Online Resource
    ISSN: 1468-8123 , 1468-8115
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2045012-6
    SSG: 13
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  • 3
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2017 ( 2017), p. 1-15
    Abstract: Li-Fei-Xiao-Yan prescription (LFXY) has been clinically used in China to treat inflammatory and infectious diseases including inflammatory lung diseases. The present study was aimed at evaluating the potential therapeutic effects and potential mechanisms of LFXY in a murine model of lipopolysaccharide- (LPS-) induced acute lung injury (ALI). In this study, the mice were orally pretreated with LFXY or dexamethasone (positive drug) before the intratracheal instillation of LPS. Our data indicated that pretreatment with LFXY enhanced the survival rate of ALI mice, reversed pulmonary edema and permeability, improved LPS-induced lung histopathology impairment, suppressed the excessive inflammatory responses via decreasing the expression of proinflammatory cytokines (TNF- α , IL-1 β , and IL-6) and chemokine (MIP-2) and inhibiting inflammatory cells migration, and repressed oxidative stress through the inhibition of MPO and MDA contents and the upregulation of antioxidants (SOD and GSH) activities. Mechanistically, treatment with LFXY significantly prevented LPS-induced TLR4 expression and NF- κ B (p65) phosphorylation. Overall, the present study suggests that LFXY protected mice from acute lung injury induced by LPS via inhibition of TLR4/NF- κ B p65 activation and upregulation of antioxidative enzymes and it may be a potential preventive and therapeutic agent for ALI in the clinical setting.
    Type of Medium: Online Resource
    ISSN: 1741-427X , 1741-4288
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2017
    detail.hit.zdb_id: 2148302-4
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