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  • Hindawi Limited  (27)
  • 1
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    Online Resource
    Curcumin Supplementation Enhances the Feeding and Growth of Largemouth Bass (Micropterus salmoides) Fed the Diet Containing 80 g/kg Fish Meal
    Hindawi Limited ; 2023
    In:  Aquaculture Research Vol. 2023 ( 2023-2-24), p. 1-10
    Details
    In: Aquaculture Research, Hindawi Limited, Vol. 2023 ( 2023-2-24), p. 1-10
    Abstract: Two feeding trials were conducted to evaluate the influence of dietary curcumin level on fish meal replacement with poultry by-product (PBM) in a largemouth bass (Micropterus salmoides) diet. In trial I, the reference diet (R) contained 400 g/kg fish meal, and PBM replaced 60 and 80% of the fish meal (P16 and P8). In trial II, the diet containing 80 g/kg fish meal (P8) served as control (C), and curcumin was added at 5000 and 10000 mg/kg (C5 and C10), respectively. Replacement of fish meal with PBM significantly influenced feed intake, phosphorus retention efficiency (PRE), body lipid content, the ratio of fish meal consumption to fish production (RCP), and wastes of carbon and phosphorus but did not result in significant alternation in weight gain, feed conversion ratio (FCR), retention efficiencies of nitrogen (NRE), carbon (CRE) and energy (ERE), viscerosomatic index (VSI), hepatosomatic index (HSI), and body composition. The RCP, body lipid content, and phosphorus waste were higher in fish fed diet R than in fish fed diets P16 and P8. Curcumin supplementation influenced weight gain, feed intake, FCR, carbon waste, and RCP, but did not result in significant alternation in CRE, NRE, PRE, ERE, condition factor, VSI, HSI, body composition, and nitrogen and phosphorus wastes. The weight gain and feed intake were higher in fish fed diet C10 than in fish fed diet C, while the carbon waste was higher in fish fed diets C5 and C10 than in fish fed diet C. Overall, the minimum dietary fish meal level for largemouth bass could be dropped to 80 g/kg when PBM was used as an alternative ingredient, and 10000 mg/kg curcumin could obviously improve growth of fish fed diet containing 80 g/kg fish meal.
    Type of Medium: Online Resource
    ISSN: 1365-2109 , 1355-557X
    URL: Article
    DOI: 10.1155/2023/5454248
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2207423-5
    detail.hit.zdb_id: 1227359-4
    detail.hit.zdb_id: 2019895-4
    SSG: 21,3
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  • 2
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    Downregulation of TNF- α /TNF-R1 Signals by AT-Lipoxin A4 May Be a Significant Mechanism of Attenuation in SAP-Associated Lung Injury
    Hindawi Limited ; 2019
    In:  Mediators of Inflammation Vol. 2019 ( 2019-04-11), p. 1-13
    Details
    In: Mediators of Inflammation, Hindawi Limited, Vol. 2019 ( 2019-04-11), p. 1-13
    Abstract: Our previous studies verified the potent anti-inflammatory effects against severe acute pancreatitis (SAP) of AT-Lipoxin A4 and their analogues. However, the anti-inflammatory effects of AT-Lipoxin A4 on SAP-associated lung injury are not thoroughly known. We used western blot, polymerase chain reaction (PCR), and immunofluorescence to investigate the downregulation of TNF- α signals in cellular and animal models of SAP-associated lung injury following AT-Lipoxin A4 intervention. In vitro , we found that AT-Lipoxin A4 markedly suppressed protein expression in TNF- α signals in human pulmonary microvascular endothelial cell, such as tumor necrosis factor receptor-associated factor 2 (TRAF2), TNF-R1-associated death domain (TRADD), receptor-interacting protein (RIP), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. Moreover, AT-Lipoxin A4 inhibited downstream signals activated by TNF- α , including NF- κ B/p65, JNK/MAPK, and ERK/MAPK. In vivo , AT-Lipoxin A4 significantly decreased pathological scores of the pancreas and lungs and the serum levels of IL-6 and TNF- α . Immunofluorescence, western blotting, and real-time PCR assay showed that AT-Lipoxin A4 significantly attenuated the expression of TNF-R1, TRADD, TRAF2, and RIP in the lungs of SAP rats. In addition, the activation of NF- κ B was also downregulated by AT-Lipoxin A4 administration as compared with SAP rats. AT-Lipoxin A4 could inhibit the production of proinflammatory mediators and activation of TNF- α downstream signals such as NF- κ B and MAPK. Downregulation of TNF- α signals by AT-Lipoxin A4 may be a significant mechanism in the attenuation of SAP-associated lung injury.
    Type of Medium: Online Resource
    ISSN: 0962-9351 , 1466-1861
    URL: Article
    DOI: 10.1155/2019/9019404
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2008065-7
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  • 3
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    Intradiscal Injection of Induced Pluripotent Stem Cell-Derived Nucleus Pulposus-Like Cell-Seeded Polymeric Microspheres Promotes Rat Disc Regeneration
    Hindawi Limited ; 2019
    In:  Stem Cells International Vol. 2019 ( 2019-05-02), p. 1-14
    Details
    In: Stem Cells International, Hindawi Limited, Vol. 2019 ( 2019-05-02), p. 1-14
    Abstract: Background . Cell replacement therapy is an attractive alternative for treating degenerated intervertebral discs (IVDs), which are related to the reduction of nucleus pulposus-like cells (NP-lCs) and the loss of the extracellular matrix. Induced pluripotent stem cells (iPSCs) which resemble embryonic stem cells are considered to be a potential resource for restoring NP-lCs and disc homeostasis. Here, we proposed an efficient two-step differentiation protocol of human iPSCs into NP-lCs and continuously tested their in vivo ability to regenerate IVDs. Methods . A polymeric gelatin microsphere (GM) was generated for sustained release of growth and differentiation factor-5 (GDF-5) and as a cell delivery vehicle of NP-lCs. By injecting NP-lC-seeded GDF-5-loaded GMs into the rat coccygeal intervertebral discs, the disc height and water content were examined with the molybdenum target radiographic imaging test and magnetic resonance imaging examination. Histology and immunohistochemistry results were shown with H & E, S-O-Fast Green, and immunohistochemistry staining. Results . We demonstrated that the injection of NP-lC-seeded GDF-5-loaded GMs could reverse IDD in a rat model. The imaging examination indicated that disc height recovered and water content increased. Histology and immunohistochemistry results indicated that the NP cells as well as their extracellular matrix were partially restored. Conclusions . The results suggest that NP-lC-seeded GDF-5-loaded GMs could partially regenerate degenerated intervertebral discs after transplantation into rat coccygeal intervertebral discs. Our study will help develop a promising method of stem cell-based therapy for IDD.
    Type of Medium: Online Resource
    ISSN: 1687-966X , 1687-9678
    URL: Article
    DOI: 10.1155/2019/6806540
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2573856-2
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  • 4
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    Effects of Transcutaneous Electrical Acupoint Stimulation on Systemic Inflammatory Response Syndrome of Patients after Percutaneous Nephrolithotomy: A Randomized Controlled Trial
    Hindawi Limited ; 2021
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2021 ( 2021-8-12), p. 1-9
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2021 ( 2021-8-12), p. 1-9
    Abstract: Purpose. Transcutaneous electrical acupoint stimulation (TEAS) is widely used. However, no study evaluated TEAS on systemic inflammatory response syndrome (SIRS) of patients after percutaneous nephrolithotomy (PCNL). The study was to evaluate TEAS on SIRS of patients after PCNL. Methods. 67 patients were enrolled and divided into group TEAS and group sham TEAS. Data were collected from 60 participants finally. In the study, TEAS or sham TEAS on bilateral Shenshu (BL23), Yinlingquan (SP9), Hegu (LI4), and Neiguan (PC6) was performed continuously throughout the procedure. The primary outcome included the incidence of systemic inflammatory response syndrome (SIRS) within 48 h after surgery. The secondary outcomes included the serum levels of inflammatory cytokines, hemodynamics changes, complications, and hospital stay after surgery. The serum levels of tumor necrosis factor- (TNF-) α and interleukin- (IL-) 6, mean arterial pressure (MAP), and heart rate (HR) at 30 min before anesthesia (T0), the time after surgery (T1), 24 h postoperation (T2), and 48 h postoperation (T3) were recorded. The consumption of analgesic during surgery was also recorded, as well as the complications and duration of hospital stay after PCNL. Results. The incidence of SIRS in group TEAS was lower than group sham TEAS (30% vs. 6.67%, p = 0.023 ). Compared with the sham TEAS group, both levels of TNF-α and IL-6 at T1, T2, and T3 were lower in the TEAS group ( p 〈 0.05 ). The levels of MAP and HR in sham TEAS at T1, T2, and T3 were markedly higher than that in the TEAS group ( p 〈 0.05 ). The total consumption of propofol and remifentanil during surgery in group TEAS was lower than that in the sham TEAS group. The incidence of hypotension, hypertension, emergence agitation, and postoperative nausea and vomiting (PONV) was also lower in group TEAS after PCNL ( p 〈 0.05 ). Conclusions. TEAS could effectively reduce the incidence of SIRS and inflammatory cytokines for patients who underwent PCNL. In addition, TEAS helped to maintain the hemodynamic stability and cut down the consumption of analgesics during PCNL, reducing the complications after PCNL.
    Type of Medium: Online Resource
    ISSN: 1741-4288 , 1741-427X
    URL: Article
    DOI: 10.1155/2021/5909956
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2148302-4
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  • 5
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    Network Pharmacology-Based Prediction of Bioactive Compounds and Potential Targets of Wenjing Decoction for Treatment of Endometriosis
    Hindawi Limited ; 2021
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2021 ( 2021-6-24), p. 1-12
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2021 ( 2021-6-24), p. 1-12
    Abstract: Endometriosis is a chronic estrogen-dependent inflammatory disorder that negatively affects the quality of life in women. The Wenjing decoction (WJD) is a traditional Chinese medicine that has been shown to have a therapeutic effect on endometriosis. Our study systematically explored the mechanism of WJD against endometriosis using a network pharmacology approach. Potentially bioactive compounds of WJD and their possible targets were retrieved from the Traditional Chinese Medicine System Pharmacology Database and Analysis Platform. The protein-protein interaction network and herbs-compounds-genes multinetwork were constructed using Cytoscape for visualization. Subsequently, the signaling pathways of common targets were retrieved from the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, and molecular docking was performed using PyRx software. In total, 48 common targets were screened, such as IL6 and ESR1, which were related to inflammation and the endocrine system. The top five bioactive compounds were quercetin, kaempferol, wogonin, beta-sitosterol, and stigmasterol. KEGG enrichment analysis revealed 65 pathways containing inflammatory- and endocrine-related signaling pathways, such as the “TNF signaling pathway” and the “estrogen signaling pathway.” Taken together, the results of our network pharmacology analysis predicted that certain active ingredients of WJD might treat endometriosis by regulating inflammation and/or endocrine, which provided references for further understanding and exploration of WJD on endometriosis.
    Type of Medium: Online Resource
    ISSN: 1741-4288 , 1741-427X
    URL: Article
    DOI: 10.1155/2021/4521843
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2148302-4
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  • 6
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    Cloud Model-Based Artificial Immune Network for Complex Optimization Problem
    Hindawi Limited ; 2017
    In:  Computational Intelligence and Neuroscience Vol. 2017 ( 2017), p. 1-17
    Details
    In: Computational Intelligence and Neuroscience, Hindawi Limited, Vol. 2017 ( 2017), p. 1-17
    Abstract: This paper proposes an artificial immune network based on cloud model (AINet-CM) for complex function optimization problems. Three key immune operators—cloning, mutation, and suppression—are redesigned with the help of the cloud model. To be specific, an increasing half cloud-based cloning operator is used to adjust the dynamic clone multipliers of antibodies, an asymmetrical cloud-based mutation operator is used to control the adaptive evolution of antibodies, and a normal similarity cloud-based suppressor is used to keep the diversity of the antibody population. To quicken the searching convergence, a dynamic searching step length strategy is adopted. For comparative study, a series of numerical simulations are arranged between AINet-CM and the other three artificial immune systems, that is, opt-aiNet, IA-AIS, and AAIS-2S. Furthermore, two industrial applications—finite impulse response (FIR) filter design and proportional-integral-differential (PID) controller tuning—are investigated and the results demonstrate the potential searching capability and practical value of the proposed AINet-CM algorithm.
    Type of Medium: Online Resource
    ISSN: 1687-5265 , 1687-5273
    URL: Article
    DOI: 10.1155/2017/5901258
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2017
    detail.hit.zdb_id: 2388208-6
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  • 7
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    Sheng Jing Decoction Can Promote Spermatogenesis and Increase Sperm Motility of the Oligozoospermia Mouse Model
    Hindawi Limited ; 2021
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2021 ( 2021-12-1), p. 1-9
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2021 ( 2021-12-1), p. 1-9
    Abstract: Sheng Jing Decoction (SJD), as a traditional Chinese medicine prescription, is mainly be used to treat male infertility. However, the pharmacological functions and molecular mechanisms of SJD are poorly understood. In this study, we investigated the functions of SJD on spermatogenesis and sperm motility and explored the potential mechanisms involved. Here, we demonstrated that high, medium, and low doses of SJD are effective in restoring the impairments of the whole body and testicular tissue by cyclophosphamide inducing and to rescue the damage of testicular tissue cells including Sertoli cells and germ cells. SJD can partly restore the decrease in sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate in oligozoospermic mouse models. Ki67 staining analyses confirm SJD can promote testicular tissue cell proliferation. Real-time RT-PCR analyses also reveal that SJD can upregulate the expression of proliferation-associated gene Lin28a and differentiation-associated genes Kit, Sohlh2, and Stra8. SJD can also reduce the impairment of mitochondrial membrane potential (MMP) and sperm plasma membrane integrity by cyclophosphamide inducing. Our results reveal that SJD is effective in improving both sperm quantity and quality by increasing the sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate. SJD can promote spermatogenesis by upregulating the expression of the proliferation-associated gene Lin28a and the differentiation-associated genes (Kit, Sohlh2, and Stra8). SJD can sustain MMP and sperm plasma membrane integrity to increase sperm motility.
    Type of Medium: Online Resource
    ISSN: 1741-4288 , 1741-427X
    URL: Article
    DOI: 10.1155/2021/3686494
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2148302-4
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  • 8
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    Salvianolic Acid B Suppresses Non-Small-Cell Lung Cancer Metastasis through PKM2-Independent Metabolic Reprogramming
    Hindawi Limited ; 2022
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2022 ( 2022-4-23), p. 1-13
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2022 ( 2022-4-23), p. 1-13
    Abstract: Objective. Salvianolic acid B (Sal B) has been demonstrated to be a potential chemoprevention agent for several cancers. Herein, we investigated the pharmacological function of Sal B on non-small-cell lung cancer (NSCLC) metastasis. Methods. Two NSCLC cell lines (NCI-H2030 and NCI-H1650) were disposed of by 200 μM Sal B or 10 μM PKM2 agonist TEPP-46. Wound healing and transwell experiments were implemented for analyzing migratory and invasive capacities. Epithelial-to-mesenchymal transition (EMT) markers β-catenin and E-cadherin were measured via western blotting. Cellular bioenergetics were evaluated with glucose uptake, lactate production, enolase activity, cellular ATP levels, as well as seahorse-based oxygen consumption rate (OCR), extracellular acidification rate (ECAR) analysis. Metabolic reprogramming markers PKM2, LDHA, and GLUT1 were detected via western blotting and immunofluorescence. Results. The results showed that Sal B disposal weakened the migration and invasion of NCI-H2030 and NCI-H1650 cells and inactivated the EMT process according to downregulation of β-catenin and upregulation of E-cadherin. Sal B-treated NSCLC cells displayed decreased glucose uptake, lactate production, enolase activity, cellular ATP levels, OCR, and ECAR, indicating a reduction in metabolic reprogramming. Additionally, Sal B downregulated the expression of PKM2, LDHA, and GLUT1. TEPP-46 may reverse the inhibitory effect of Sal B on metastasis as well as metabolic reprogramming. Conclusion. Our findings provide evidence that Sal B enables to weaken NSCLC metastasis through PKM2-independent metabolic reprogramming, which sheds light on the promising therapeutic usage of Sal B in treating NSCLC.
    Type of Medium: Online Resource
    ISSN: 1741-4288 , 1741-427X
    URL: Article
    DOI: 10.1155/2022/9302403
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2148302-4
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  • 9
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    An Improved Nonhomogeneous Discrete Grey Model and Its Application
    Hindawi Limited ; 2020
    In:  Mathematical Problems in Engineering Vol. 2020 ( 2020-05-08), p. 1-8
    Details
    In: Mathematical Problems in Engineering, Hindawi Limited, Vol. 2020 ( 2020-05-08), p. 1-8
    Abstract: In recent years, the nonhomogeneous grey model has received much attention owing to its flexibility and applicability of forecasting small samples. To improve further the prediction accuracy of the nonhomogeneous grey model, this paper is to introduce a new whitening equation with variable coefficient into the original nonhomogeneous grey model, which is abbreviated as ONGM 1,1 , k , c . First of all, the detailed computational steps of the time response function of the novel model and the restored values of the raw data sequence are deduced through grey modelling techniques. Secondly, two empirical examples from the previous literature are conducted to prove the validity of the novel model. Finally, the novel model is applied to forecast natural gas demand of China, and the results show that the novel model has a better prediction performance compared with other commonly used grey models, including GM 1,1 , DGM 1,1 , NGM 1,1 , k , c , and NGBM 1,1 .
    Type of Medium: Online Resource
    ISSN: 1024-123X , 1563-5147
    URL: Article
    DOI: 10.1155/2020/4638296
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2014442-8
    SSG: 11
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  • 10
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    CD73 (NT5E) Promotes the Proliferation and Metastasis of Lung Adenocarcinoma through the EGFR/AKT/mTOR Pathway
    Hindawi Limited ; 2022
    In:  BioMed Research International Vol. 2022 ( 2022-6-29), p. 1-12
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2022 ( 2022-6-29), p. 1-12
    Abstract: Introduction. Lung cancer is the most common malignant tumor and the main cause of tumor-related death globally. As the 5-year survival rate of lung adenocarcinoma (LUAD) remains low, it is necessary to investigate novel molecular markers and therapeutic targets for LUAD. Materials and Methods. The protein expression of CD73 (NT5E) in LUAD specimens was analyzed using immunohistochemistry. Reverse transcription-quantitative PCR and western blot analysis were used to analyze the mRNA and protein expression levels of several genes in LUAD cells. The proliferation of LUAD cells was evaluated using proliferation and colony formation assays and apoptosis analysis. Wound healing and Transwell invasion assays were used to analyze the migration and invasion of the A549 cells, respectively. In addition, overexpression plasmids and small interfering RNAs were used to overexpress or knockdown the expression levels of CD73 in the A549 cell line, respectively. Finally, the interaction between CD73 and EGFR in the A549 cell line was analyzed using immunoprecipitation. Results. Our research emphasized the importance of CD73 in the prognosis of LUAD and highlighted it as a potential therapeutic target. We also found that the mRNA and protein expression levels of CD73 are increased in LUAD specimens and cell lines and were associated with a poor prognosis in patients with LUAD. Furthermore, it was revealed that CD73 may promote the proliferation, migration, and invasion of the A549 cell line. Finally, we demonstrated that CD73 could bind epidermal growth factor receptor (EGFR) to further regulate the activation of the AKT/mTOR signaling pathway. Conclusions. CD73 promotes LUAD proliferation and metastasis through EGFR/AKT/mTOR axis.
    Type of Medium: Online Resource
    ISSN: 2314-6141 , 2314-6133
    URL: Article
    DOI: 10.1155/2022/9944847
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2698540-8
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