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  • 1
    Online Resource
    Online Resource
    Network Pharmacology and Bioinformatics Approach Reveals the Therapeutic Mechanism of Action of Baicalein in Hepatocellular Carcinoma
    Hindawi Limited ; 2019
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2019 ( 2019-02-12), p. 1-15
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2019 ( 2019-02-12), p. 1-15
    Abstract: Liver cancer is the fourth leading cause of cancer death worldwide, and hepatocellular carcinoma (HCC) accounts for the greatest proportion of these deaths. Baicalein, a flavonoid isolated from the root of Scutellariae radix , is considered a potential candidate to treat HCC. However, the underlying molecular mechanisms remain poorly understood. In the present study, a network pharmacological approach was combined with microarray data (GSE95504) acquired from the Gene Expression Omnibus database to reveal the therapeutic mechanisms of action of baicalein at a systemic level. We identified 38 baicalein targets and 76 differently expressed genes (DEGs) following treatment with baicalein, including 55 upregulated and 21 downregulated genes. The DEGs were significantly enriched in the biological functions of apoptosis, endoplasmic reticulum stress, and PERK-mediated unfolded protein response. Protein-protein interaction (PPI) network construction and topological screening revealed a core module of PPIs including two baicalein targets, TP53 and CDK1 , and two downregulated DEGs, HSPA1A and HSPA1B . Expression and survival data for these genes in the module derived from Gene Expression Profiling Interactive Analysis (GEPIA) were subjected to Kaplan–Meier analysis of overall survival and disease-free survival. Overexpression of CDK1 , BRCA1 , TUBB , HSPA1A , HSPA1B , and HSPA4 was associated with significantly worse overall survival, while overexpression of CDK1 , CLU7 , BRCA1 , and TUBB was associated with significantly worse disease-free survival. These data suggest that baicalein exerts therapeutic effects against HCC via a PPI network involving TP53, CDK1, HSPA1A, and HSPA1B.
    Type of Medium: Online Resource
    ISSN: 1741-427X , 1741-4288
    URL: Article
    DOI: 10.1155/2019/7518374
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2148302-4
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  • 2
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    Efficacy of Balloon Guide Catheter-Assisted Thrombus Repair in Stroke Treatment: A Retrospective Survey in China
    Hindawi Limited ; 2022
    In:  BioMed Research International Vol. 2022 ( 2022-9-28), p. 1-7
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2022 ( 2022-9-28), p. 1-7
    Abstract: Background. The first-pass (FP) effect, defined by successful cerebral reperfusion from a single pass of an endovascular stentriever, was associated with shorter procedural times and possible improved outcomes in patients with ischemic stroke secondary to large vessel occlusion. The adjunctive use of balloon guide catheter (BGC) may increase the rates of the first-pass effect. In this retrospective study we examined the impact of BGC on the first-pass effect in acute stroke patients. Methods. We included patients with acute ischemic stroke with large vessel occlusion treated by endovascular thrombectomy from 2018 to 2019. We categorized the cases into BGC and non-BGC groups. Differences in time metrics and outcomes were compared. Result. One hundred and thirty-two patients were included, and sixty-two were in BGC group (47.0%). The median procedural time was shorter (83.0 minutes vs 120.0 minutes, P = 0.000 ), and FP rate was higher in BGC group (58.1% vs 32.9%, P = 0.004 ) compared with non-BGC group. Proportion of modified Thrombolysis in Cerebral Infarction (mMTICI) 3 was higher (66.1% vs 37.1%, P = 0.001 ), and modified Rankin Scale (mRS) 0 to 2 was higher (59.7% vs 41.4%, P = 0.036 ) in BGC group compared with non-BGC group. In addition, BGC was associated with successful reperfusion odds ratio, 0.383; 95% confidence interval: 0.174-0.847; P = 0.018 ). The FP rate of BGC in the distal ICA was higher than that in the proximal ICA (87.5% vs 39.5%, P = 0.000 ), and the good clinical outcome rate at 90 days in the distal ICA was also higher than that in the proximal ICA (91.7% vs 39.5%, P = 0.000 ). Conclusion. We showed that BGC shortened the procedural time and increased the rate of the successful FP. We recommend that BGC could be considered the preferred technique for endovascular intervention in stroke.
    Type of Medium: Online Resource
    ISSN: 2314-6141 , 2314-6133
    URL: Article
    DOI: 10.1155/2022/4278048
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2698540-8
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  • 3
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    Genetic diversity of porcine circovirus type 2 in China between 1999–2017
    Hindawi Limited ; 2019
    In:  Transboundary and Emerging Diseases Vol. 66, No. 1 ( 2019-01), p. 599-605
    Details
    In: Transboundary and Emerging Diseases, Hindawi Limited, Vol. 66, No. 1 ( 2019-01), p. 599-605
    Type of Medium: Online Resource
    ISSN: 1865-1674 , 1865-1682
    URL: Issue
    URL: Article
    DOI: 10.1111/tbed.2019.66.issue-1
    DOI: 10.1111/tbed.13040
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2414822-2
    SSG: 22
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  • 4
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    miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3
    Hindawi Limited ; 2021
    In:  Stem Cells International Vol. 2021 ( 2021-7-15), p. 1-18
    Details
    In: Stem Cells International, Hindawi Limited, Vol. 2021 ( 2021-7-15), p. 1-18
    Abstract: Objective. Accumulating evidence indicates that microRNAs (miRNAs) play crucial roles in osteogenic differentiation. However, the associated mechanisms remain elusive. This paper is aimed at exploring the role of miR-129-5p in regulating bone marrow mesenchymal stem cell (BMSC) differentiation and bone regeneration in vivo and in vitro. Methods. BMSCs were transduced by miR-129-5p mimic, miR-129-5p inhibitor, and negative control lentivirus. The ability of BMSC differentiation to osteoblast was tested by alkaline phosphatase (ALP) and alizarin red staining (ARS). The expression of osteogenic genes (Runx2, Bmp2, and OCN) was examined via quantitative RT-PCR and western blot. A mouse model of calvaria defect was investigated by Micro-CT, immunohistochemistry, and histological examination. The luciferase reporter gene assay was performed to confirm the binding between Dkk3 and miR-129-5p. For the transfection experiments, lipofectamine 3000 was used to transfect pcDNA-Dkk3 into BMSCs to overexpress Dkk3. Coimmunoprecipitation and immunofluorescent localization assay were included for exploring the role of Dkk3 and β-catenin. Results. miR-129-5p was induced in BMSCs and MSC cell line C3H10T1/2 cells under osteogenic medium. Overexpression of miR-129-5p significantly promoted osteogenic differentiation of BMSCs in vitro. Moreover, BMSCs transduced with miR-129-5p mimic exhibited better bone regeneration compared with BMSCs transduced with control counterpart in vivo. Luciferase and western blot data showed that Dickkopf3 (Dkk3) is a target gene of miR-129-5p and the expression of Dkk3 was inhibited in BMSCs transduced with miR-129-5p mimic but enhanced in BMSCs transduced with miR-129-5p inhibitor. In addition, Dkk3 interacted with β-catenin directly. Conclusions. miR-129-5p promotes osteogenic differentiation of BMSCs and bone regeneration, and miR-129-5p/Dkk3 axis may be new potential targets for the treatment of bone defect and bone loss.
    Type of Medium: Online Resource
    ISSN: 1687-9678 , 1687-966X
    URL: Article
    DOI: 10.1155/2021/7435605
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2573856-2
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  • 5
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    Online Resource
    Differences and Correlations of Anxiety, Sleep Quality, and Pressure-Pain Threshold between Patients with Chronic Low Back Pain and Asymptomatic People
    Hindawi Limited ; 2022
    In:  Pain Research and Management Vol. 2022 ( 2022-5-17), p. 1-7
    Details
    In: Pain Research and Management, Hindawi Limited, Vol. 2022 ( 2022-5-17), p. 1-7
    Abstract: Background. Chronic low back pain (CLBP) is a clinically common and expensive disease. Patients frequently take sick leaves because of pain and dysfunction, and their unpleasant life and work experiences cause psychological depression and anxiety and affect their quality of life. Sleep disturbance is a common problem among patients with low back pain (LBP) with more than 50% complaining about poor sleep quality. This study aimed to explore the correlations between anxiety, sleep quality, and pressure-pain threshold (PPT) and their differences between patients with CLBP and asymptomatic people. Methods. Forty patients with CLBP and 40 asymptomatic people were recruited. Relevant data, including State-Trait Anxiety Inventory, Pittsburgh Sleep Quality Index, and PPT, were individually and independently collected by blinded physiotherapists with a practicing certificate and then statistically analyzed. An independent sample t-test was used to determine the intergroup differences between patients with CLBP and asymptomatic populations. Pearson correlation coefficient was employed for correlation analysis. Results. The CLBP group had significantly higher anxiety scores (41.64 ± 9.88 vs. 36.69 ± 8.31; t = −2.496, p = 0.015 ) than the asymptomatic group. A significant difference was found in the total score of the Pittsburgh Sleep Quality Index (6.41 ± 2.43 vs. 5.09 ± 2.18; t = −2.628, p = 0.010 ) but not in the trait anxiety (44.00 ± 7.83 vs. 42.67 ± 9.51; t = −0.695, p = 0.489 ) of the two groups. State−Trait Anxiety Inventory showed a low to moderate negative correlation with PPT. No remarkable correlation was observed between Pittsburgh Sleep Quality Index and PPT. Conclusions. Patients with CLBP showed considerably worse state anxiety and sleep quality than asymptomatic people; however, no substantial difference in PPT was found between the two groups. The results suggest that in clinical practice, the focus should include pain and related social and psychological factors. CLBP treatment could be considered from multiple perspectives and disciplines.This trial is registered with Chinese Clinical Trial Registry (Trial registration: ChiCTR-TRC-13003701).
    Type of Medium: Online Resource
    ISSN: 1918-1523 , 1203-6765
    URL: Article
    DOI: 10.1155/2022/8648584
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2048409-4
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  • 6
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    Online Resource
    TRPV1 and PLC Participate in Histamine H4 Receptor-Induced Itch
    Hindawi Limited ; 2016
    In:  Neural Plasticity Vol. 2016 ( 2016), p. 1-9
    Details
    In: Neural Plasticity, Hindawi Limited, Vol. 2016 ( 2016), p. 1-9
    Abstract: Histamine H4 receptor has been confirmed to play a role in evoking peripheral pruritus. However, the ionic and intracellular signaling mechanism of activation of H4 receptor on the dorsal root ganglion (DRG) neurons is still unknown. By using cell culture and calcium imaging, we studied the underlying mechanism of activation of H4 receptor on the DRG neuron. Immepip dihydrobromide (immepip)—a histamine H4 receptor special agonist under cutaneous injection—obviously induced itch behavior of mice. Immepip-induced scratching behavior could be blocked by TRPV1 antagonist AMG9810 and PLC pathway inhibitor U73122. Application of immepip (8.3–50  μ M) could also induce a dose-dependent increase in intracellular Ca 2+ ( C a 2 + i ) of DRG neurons. We found that 77.8% of the immepip-sensitized DRG neurons respond to the TRPV1 selective agonist capsaicin. U73122 could inhibit immepip-induced Ca 2+ responses. In addition, immepip-induced C a 2 + i increase could be blocked by ruthenium red, capsazepine, and AMG9810; however it could not be blocked by TRPA1 antagonist HC-030031. These results indicate that TRPV1 but not TRPA1 is the important ion channel to induce the DRG neurons’ responses in the downstream signaling pathway of histamine H4 receptor and suggest that TRPV1 may be involved in the mechanism of histamine-induced itch response by H4 receptor activation.
    Type of Medium: Online Resource
    ISSN: 2090-5904 , 1687-5443
    URL: Article
    DOI: 10.1155/2016/1682972
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2016
    detail.hit.zdb_id: 2236872-3
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  • 7
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    Distribution Property of Shear Strength Parameters of Q2 and Q3 Loess in Northwest China and Its Application in Reliability Analysis of Natural and Filled Slopes
    Hindawi Limited ; 2021
    In:  Advances in Civil Engineering Vol. 2021 ( 2021-2-10), p. 1-9
    Details
    In: Advances in Civil Engineering, Hindawi Limited, Vol. 2021 ( 2021-2-10), p. 1-9
    Abstract: Geological materials have randomness in nature. Statistical analysis can help revealing the variation pattern of the material parameters and the reliability of the slope. In this paper, the probability distribution and variability of shear strength parameters of typical Q2 and Q3 loess in Northwest China were statistically analysed using the collected geological survey data in Yan`an, Shaanxi Province. Resulting probability properties were applied to the reliability analysis for the natural and fill slope in loess area using the Monte Carlo Method. Finally, an optimization analysis was carried out for the fill slope in the background engineering project. Research results show that normal distribution and lognormal distribution can be used to describe the statistical properties of c and φ of Q2 and Q3 loess. Variance of c is large while the variability of φ is relatively small. The influence of the variability of φ on the reliability index of loess natural slope is generally larger than that of c. On the contrary, influence of the variability of c on the reliability index of the fill slope is generally greater than that of φ, which is related to the structural and uniformity of the soil. Finally, relation between slope height and minimum gradient coefficient is linear when the failure probability of fill slope is less than 10%. Results in this study can be used as a reference for the slope design and construction for the cut and fill engineering in loess area.
    Type of Medium: Online Resource
    ISSN: 1687-8094 , 1687-8086
    URL: Article
    DOI: 10.1155/2021/6671233
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2449760-5
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  • 8
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    A Combined Water Extract of Frankincense and Myrrh Alleviates Neuropathic Pain in Mice via Modulation of TRPV1
    Hindawi Limited ; 2017
    In:  Neural Plasticity Vol. 2017 ( 2017), p. 1-11
    Details
    In: Neural Plasticity, Hindawi Limited, Vol. 2017 ( 2017), p. 1-11
    Abstract: Frankincense and myrrh are widely used in clinics as a pair of herbs to obtain a synergistic effect for relieving pain. To illuminate the analgesia mechanism of frankincense and myrrh, we assessed its effect in a neuropathic pain mouse model. Transient receptor potential vanilloid 1 (TRPV1) plays a crucial role in neuropathic pain and influences the plasticity of neuronal connectivity. We hypothesized that the water extraction of frankincense and myrrh (WFM) exerted its analgesia effect by modulating the neuronal function of TRPV1. In our study, WFM was verified by UHPLC-TQ/MS assay. In vivo study showed that nociceptive response in mouse by heat and capsaicin induced were relieved by WFM treatment. Furthermore, thermal hypersensitivity and mechanical allodynia were also alleviated by WFM treatment in a chronic constriction injury (CCI) mouse model. CCI resulted in increased TRPV1 expression at both the mRNA and protein levels in predominantly small-to-medium neurons. However, after WFM treatment, TRPV1 expression was reverted in real-time PCR, Western blot, and immunofluorescence experiments. Calcium response to capsaicin was also decreased in cultured DRG neurons from CCI model mouse after WFM treatment. In conclusion, WFM alleviated CCI-induced mechanical allodynia and thermal hypersensitivity via modulating TRPV1.
    Type of Medium: Online Resource
    ISSN: 2090-5904 , 1687-5443
    URL: Article
    DOI: 10.1155/2017/3710821
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2017
    detail.hit.zdb_id: 2236872-3
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  • 9
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    A Systems Pharmacology-Based Study of the Molecular Mechanisms of San Cao Decoction for Treating Hypertension
    Hindawi Limited ; 2019
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2019 ( 2019-07-02), p. 1-10
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2019 ( 2019-07-02), p. 1-10
    Abstract: Traditional Chinese medicine (TCM) has a longstanding history and has gained widespread clinical applications. San Cao Decoction (SCD) is an experience prescription first formulated by Prof. Duzhou Liu. We previously demonstrated its antihypertensive effects; however, to systematically explain the underlying mechanisms of action, we employed a systems pharmacology approach for pharmacokinetic screening and target prediction by constructing protein-protein interaction networks of hypertension-related and putative SCD-related targets, and Database for Annotation, Visualization, and Integrated Discovery enrichment analysis. We identified 123 active compounds in SCD and 116 hypertension-related targets. Furthermore, the enrichment analysis of the drug-target network showed that SCD acts in a multidimensional manner to regulate PI3K-Akt-endothelial nitric oxide synthase signaling to maintain blood pressure. Our results highlighted the molecular mechanisms of antihypertensive actions of medicinal herbs at a systematic level.
    Type of Medium: Online Resource
    ISSN: 1741-427X , 1741-4288
    URL: Article
    DOI: 10.1155/2019/3171420
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2148302-4
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  • 10
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    The Time Course of Perceptual Closure of Incomplete Visual Objects: An Event-Related Potential Study
    Hindawi Limited ; 2020
    In:  Computational Intelligence and Neuroscience Vol. 2020 ( 2020-10-6), p. 1-7
    Details
    In: Computational Intelligence and Neuroscience, Hindawi Limited, Vol. 2020 ( 2020-10-6), p. 1-7
    Abstract: Perceptual organization is an important part of visual and auditory information processing. In the case of visual occlusion, whether the loss of information in images could be recovered and thus perceptually closed affects object recognition. In particular, many elderly subjects have defects in object recognition ability, which may be closely related to the abnormalities of perceptual functions. This phenomenon even can be observed in the early stage of dementia. Therefore, studying the neural mechanism of perceptual closure and its relationship with sensory and cognitive processing is important for understanding how the human brain recognizes objects, inspiring the development of neuromorphic intelligent algorithms of object recognition. In this study, a new experiment was designed to explore the realistic process of perceptual closure under occlusion and intact conditions of faces and building. The analysis of the differences in ERP components P1, N1, and Ncl indicated that the subjective awareness of perceptual closure mainly occurs in Ncl, but incomplete information has been processed and showed different manners compared to complete stimuli in N170 for facial materials. Although occluded, faces, but not buildings, still maintain the specificity of perceptual processing. The Ncl by faces and buildings did not show significant differences in both amplitude and latency, suggesting a “completing” process regardless of categorical features.
    Type of Medium: Online Resource
    ISSN: 1687-5273 , 1687-5265
    URL: Article
    DOI: 10.1155/2020/8825197
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2388208-6
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