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  • 1
    Online Resource
    Online Resource
    Humic Acids Inhibit Platelet Activation to Reduce Venous Thromboembolism in Mice
    Hindawi Limited ; 2022
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2022 ( 2022-12-21), p. 1-9
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2022 ( 2022-12-21), p. 1-9
    Abstract: Objective. We aimed to investigate the effects of the natural product humic acids (HA) on platelet activation and development of venous thromboembolism (VTE) in mice and further explore the relevant mechanism. Methods. Eight-week C57BL/6 mice were randomly assigned to three groups: sham operation group (n = 7), VTE group (n = 8), and VTE + HA group (n = 10). Thrombi were harvested to hematoxylin-eosin staining to evaluate the thrombolysis and recanalization of the thrombus. In addition, flow cytometry was performed to detect the expression levels of protein disulfide isomerase on endothelial-derived exosomes and glycoprotein IIb/IIIa on the surface of the activated platelets surface in plasma. Furthermore, the protein expression level of glycoprotein IIb/IIIa in thrombus was determined by immunohistochemistry and immunofluorescence. Results. The length of thrombosis in the VTE + HA group was significantly shorter than that in the VTE group ( P = 0.040 ). No significant differences were observed in thrombolysis and recanalization between the VTE + HA group and the VTE group ( P 〉 0.05 ). The content of protein disulfide isomerase carried by endothelial-derived exosomes was significantly increased in the VTE group ( P = 0.008 ) but significantly reduced by native humic acids ( P = 0.012 ). Compared with the VTE group, the expression of glycoprotein IIb/IIIa on activated platelet surface in the VTE + HA group was significantly decreased ( P = 0.002 ). The concentration of plasmatic P-selectin in the VTE group was significantly higher than that in the VTE + HA group ( P 〈 0.001 ). Conclusion. We demonstrate that HA possess a pharmacological property that decreases venous thrombus formation in mice. The underlying mechanism is that HA could inhibit the expression of glycoprotein IIb/IIIa on the activated platelets surface by suppressing endothelial-derived exosomes carrying on protein disulfide isomerase, thereby blocking platelet activation.
    Type of Medium: Online Resource
    ISSN: 1741-4288 , 1741-427X
    URL: Article
    DOI: 10.1155/2022/6606423
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2148302-4
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  • 2
    Online Resource
    Online Resource
    MXRA5 Is a Novel Immune-Related Biomarker That Predicts Poor Prognosis in Glioma
    Hindawi Limited ; 2021
    In:  Disease Markers Vol. 2021 ( 2021-6-9), p. 1-13
    Details
    In: Disease Markers, Hindawi Limited, Vol. 2021 ( 2021-6-9), p. 1-13
    Abstract: Background. Glioma is the most common primary intracranial tumor and is associated with poor prognosis. Identifying effective biomarkers for glioma is particularly important. MXRA5, a secreted glycoprotein, is involved in cell adhesion and extracellular matrix remodeling and has been reported to be expressed in many cancers. However, the role and mechanism of action of MXRA5 in gliomas remain unclear. This study was aimed at investigating the role of MXRA5 at the transcriptome level and its clinical prognostic value. Methods. In this study, RNA microarray data of 301 glioma patients from the Chinese Glioma Genome Atlas (CGGA) were collected as a training cohort and RNA-seq data of 702 glioma samples from The Cancer Genome Atlas (TCGA) were used for validation. We analyzed the clinical and molecular characteristics as well as the prognostic value of MXRA5 in glioma. In addition, the expression level of MXRA was evaluated in 28 glioma tissue samples. Results. We found that MXRA5 expression was significantly upregulated in high-grade gliomas and IDH wild-type gliomas compared to controls. Receiver operating characteristic (ROC) analysis showed that MXRA5 is a potential marker of the mesenchymal subtype of glioblastoma multiforme (GBM). We found that MXRA5 expression is highly correlated with immune checkpoint molecule expression levels and tumor-associated macrophage infiltration. High MXRA5 expression could be used as an independent indicator of poor prognosis in glioma patients. Conclusion. Our study suggests that MXRA5 expression is associated with the clinicopathologic features and poor prognosis of gliomas. MXRA5 may play an important role in the immunosuppressive microenvironment of glioma. As a secreted glycoprotein, MXRA5 is a potential circulating biomarker for glioma, deserving further investigation.
    Type of Medium: Online Resource
    ISSN: 1875-8630 , 0278-0240
    URL: Article
    DOI: 10.1155/2021/6680883
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2033253-1
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  • 3
    Online Resource
    Online Resource
    The Effects of the Context-Dependent Codon Usage Bias on the Structure of the nsp1 α of Porcine Reproductive and Respiratory Syndrome Virus
    Hindawi Limited ; 2014
    In:  BioMed Research International Vol. 2014 ( 2014), p. 1-10
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2014 ( 2014), p. 1-10
    Abstract: The information about the crystal structure of porcine reproductive and respiratory syndrome virus (PRRSV) leader protease nsp1 α is available to analyze the roles of tRNA abundance of pigs and codon usage of the nsp1 α gene in the formation of this protease. The effects of tRNA abundance of the pigs and the synonymous codon usage and the context-dependent codon bias (CDCB) of the nsp1 α on shaping the specific folding units ( α -helix, β -strand, and the coil) in the nsp1 α were analyzed based on the structural information about this protease from protein data bank (PDB: 3IFU) and the nsp1 α of the 191 PRRSV strains. By mapping the overall tRNA abundance along the nsp1 α , we found that there is no link between the fluctuation of the overall tRNA abundance and the specific folding units in the nsp1 α , and the low translation speed of ribosome caused by the tRNA abundance exists in the nsp1 α . The strong correlation between some synonymous codon usage and the specific folding units in the nsp1 α was found, and the phenomenon of CDCB exists in the specific folding units of the nsp1 α . These findings provide an insight into the roles of the synonymous codon usage and CDCB in the formation of PRRSV nsp1 α structure.
    Type of Medium: Online Resource
    ISSN: 2314-6133 , 2314-6141
    URL: Article
    DOI: 10.1155/2014/765320
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2014
    detail.hit.zdb_id: 2698540-8
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