In:
Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2020 ( 2020-06-22), p. 1-9
Abstract:
This study aimed to explore the protective effects of Wenshen-Yanggan decoction on dopaminergic (DA) neuron injury in a rotenone-induced mouse model with chronic Parkinson’s disease (PD) and explore its mechanism of action. Ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to measure the content of six main components in the Wenshen-Yanggan decoction. The chronic PD mouse model was established by treating 10-month-old healthy wild C57BL/6 male mice with rotenone 30 mg/kg/day for 28 days in succession. The pole test and rotarod test were applied to detect the rescue effect of Wenshen-Yanggan decoction in high, medium, and low dosages, respectively, on PD-like behaviors in mice with chronic PD. The protective effect of Wenshen-Yanggan decoction on the mesencephalic nigrostriatal DA neuron injury was determined employing tyrosine hydroxylase (TH) immunofluorescence staining. Enzyme-linked immunosorbent assay (ELISA) was adopted to measure the inflammatory cytokines in serum, including TNF- α (tumor necrosis factor-alpha), IFN- γ (interferon gamma), NF- κ B (nuclear factor kappa-B), and IL-1 β (interleukin-1 beta). Western blotting was performed to quantify the expression of phosphorylated c -Jun N-terminal kinase ( p -JNK), cleaved caspase-3, B-cell lymphoma-2 (Bcl-2), and NF- κ B in the brain. Our results showed that the Wenshen-Yanggan decoction in high, medium, and low dosages reduced the turning time of mice P 〈 0.01 , P 〈 0.01 , and P 〈 0.05 . The high and medium dosages shortened the total climbing time of PD mice in the pole test P 〈 0.01 and P 〈 0.05 . Meanwhile, the high, medium, and low dosages increased the rod-standing time of PD mice in the rotarod test P 〈 0.01 , P 〈 0.05 , and P 〈 0.05 . Besides, the decoction reversed the decrease in TH-positive neurons induced by rotenone, upregulated TH protein expression, and downregulated the α -syn expression in the PD model. Moreover, the decoction in high dosage significantly inhibited the expression of p -JNK, cleaved caspase-3, and NF- κ B in the midbrain of PD mice P 〈 0.05 , P 〈 0.05 , and P 〈 0.01 , upregulated the expression of Bcl-2 P 〈 0.05 , and decreased the content of TNF- α , IFN- γ , NF- κ B, and IL-1 β in the serum P 〈 0.001 , P 〈 0.001 , P 〈 0.001 , and P 〈 0.001 . Taken together, the Wenshen-Yanggan decoction could protect mice from rotenone-induced chronic PD, which might be related to the reduction of the DA neuron apoptosis via suppressing the inflammatory reaction and the neuronal apoptosis pathway.
Type of Medium:
Online Resource
ISSN:
1741-427X
,
1741-4288
DOI:
10.1155/2020/9838295
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2020
detail.hit.zdb_id:
2148302-4
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