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  • Hindawi Limited  (4)
  • 1
    Online Resource
    Online Resource
    Circulating T Cells Exhibit Different TIM3/Galectin-9 Expression in Patients with Obesity and Obesity-Related Diabetes
    Hindawi Limited ; 2020
    In:  Journal of Diabetes Research Vol. 2020 ( 2020-10-15), p. 1-10
    Details
    In: Journal of Diabetes Research, Hindawi Limited, Vol. 2020 ( 2020-10-15), p. 1-10
    Abstract: Aims. Obesity is highly associated with type 2 diabetes mellitus (T2DM). The TIM3/galectin-9 pathway plays an important role in immune tolerance. Herein, we aimed to investigate the expression of TIM3 and galectin-9 in peripheral blood and to evaluate their clinical significance in patients with obesity and obesity-related T2DM. Methods. We performed flow cytometry on peripheral blood samples from healthy donors (HC), patients with simple obesity (OB), and patients with obesity comorbid T2DM (OD). The expression of TIM3 on CD3+, CD4+, and CD8+ T cells was determined. The level of galectin-9 in plasma was detected by ELISA. Results. We demonstrated the enhancement of TIM3 on CD3+, CD4+, and CD8+ T cells in the OB group when compared with healthy controls, while it was decreased significantly in the OD group. The TIM3+CD8+ T cells of the OB group were positively correlated with risk factors including BMI, body fat rate, and hipline. The concentration of galectin-9 of the OD group in plasma was significantly higher than that of healthy donors and the OB group. Moreover, the level of galectin-9 of the OD group was positively correlated with fasting insulin and C-peptide, which were two clinical features that represented pancreatic islet function in T2DM. Conclusions. Our results suggested that TIM3 and galectin-9 may be potential biomarkers related to the pathogenesis of obesity-related T2DM.
    Type of Medium: Online Resource
    ISSN: 2314-6753 , 2314-6745
    URL: Article
    DOI: 10.1155/2020/2583257
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2711897-6
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  • 2
    Online Resource
    Online Resource
    Identification of Cuproptosis-Related Genes in Nonalcoholic Fatty Liver Disease
    Hindawi Limited ; 2023
    In:  Oxidative Medicine and Cellular Longevity Vol. 2023 ( 2023-2-21), p. 1-18
    Details
    In: Oxidative Medicine and Cellular Longevity, Hindawi Limited, Vol. 2023 ( 2023-2-21), p. 1-18
    Abstract: Nonalcoholic fatty liver disease (NAFLD) is the most prevalent hepatic pathology worldwide. However, the precise molecular mechanisms for NAFLD are still not sufficiently explained. Recently, a new mode of cell death (cuproptosis) is found. However, the relationship between NAFLD and cuproptosis remains unclear. We analyzed three public datasets (GSE89632, GSE130970, and GSE135251) to identify cuproptosis-related genes stably expressed in NAFLD. Then, we performed a series of bioinformatics analyses to explore the relationship between NAFLD and cuproptosis-related genes. Finally, 6 high-fat diet- (HFD-) induced NAFLD C57BL/6J mouse models were established to carry out transcriptome analysis. The results of gene set variation analysis (GSVA) revealed that the cuproptosis pathway was abnormally activated to a certain degree ( p = 0.035 in GSE89632, p = 0.016 in GSE130970, p = 0.22 in GSE135251), and the principal component analysis (PCA) of the cuproptosis-related genes showed that the NAFLD group separated from the control group, with the first two principal components accounting for 58.63%-74.88% of the variation. Among three datasets, two cuproptosis-related genes (DLD and PDHB, p 〈 0.01 or 0.001) were stably upregulated in NAFLD. Additionally, both DLD ( AUC = 0.786 – 0.856 ) and PDHB ( AUC = 0.771 – 0.836 ) had favorable diagnostic properties, and the multivariate logistics regression model further improved the diagnostic properties ( AUC = 0.839 – 0.889 ). NADH, flavin adenine dinucleotide, and glycine targeted DLD, and pyruvic acid and NADH targeted PDHB in the DrugBank database. The DLD and PDHB were also associated with clinical pathology, especially with steatosis (DLD, p = 0.0013 – 0.025 ; PDHB, p = 0.002 – 0.0026 ) and NAFLD activity score (DLD, p = 0.004 – 0.02 ; PDHB, p = 0.003 – 0.031 ). What is more, DLD and PDHB were correlated with stromal score (DLD, R = 0.38 , p 〈 0.001 ; PDHB, R = 0.31 , p 〈 0.001 ) and immune score (DLD, R = 0.26 , p 〈 0.001 ; PDHB, R = 0.27 , p 〈 0.001 ) in NAFLD. Furthermore, Dld and Pdhb were also significantly upregulated in the NAFLD mouse model. In conclusion, cuproptosis pathways, especially DLD and PDHB, could be potential candidate genes for NAFLD diagnostic and therapeutic options.
    Type of Medium: Online Resource
    ISSN: 1942-0994 , 1942-0900
    URL: Article
    DOI: 10.1155/2023/9245667
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2455981-7
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  • 3
    Online Resource
    Online Resource
    Characterization of Soft Contact Lens Edge Fitting during Daily Wear Using Ultrahigh-Resolution Optical Coherence Tomography
    Hindawi Limited ; 2018
    In:  Journal of Ophthalmology Vol. 2018 ( 2018), p. 1-6
    Details
    In: Journal of Ophthalmology, Hindawi Limited, Vol. 2018 ( 2018), p. 1-6
    Abstract: Purpose . To determine conjunctival overlap over the edge of soft contact lens and to visualize the peripheral postlens tear film (PoLTF) underneath soft contact lenses using ultrahigh-resolution optical coherence tomography (UHR-OCT). Methods . Twenty participants (4 males and 16 females, 23.0 ± 3.7 years) were fitted with two different types of soft contact lenses randomly. The limbus with lens was imaged with the UHR-OCT at the horizontal meridian every two hours up to 6 hours during lens wear. The conjunctival overlap was ranked as the percentage of the edge covered by the conjunctiva. The frequency of occurrence for visualized peripheral PoLTF was determined. Results . The average conjunctival overlaps at insertion were 49% and 73% for galyfilcon A and balafilcon A lenses and increased significantly to 84% and 90% by 6 hours of lens wear ( P 〈 0.001 ). Lenses with rounded edges had more conjunctival overlap than the lenses with angled edges ( P = 0.014 ). There were significant decreases for PoLTF on the conjunctiva ( P = 0.014 ) and peripheral cornea ( P = 0.004 ) over the study period compared to insertion. The percentage of subjects with PoLTF on the conjunctiva (32.5%) and peripheral cornea (36%) were greater in subjects wearing balafilcon A lenses ( P = 0.017 ). Conclusions . Increased conjunctival overlap over the lens edges and reduced PoLTF underneath the peripheral region of soft contact lenses were shown during lens daily wear. The lens edge configuration may play a role in conjunctival response and peripheral PoLTF.
    Type of Medium: Online Resource
    ISSN: 2090-004X , 2090-0058
    URL: Article
    DOI: 10.1155/2018/3463595
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2018
    detail.hit.zdb_id: 2546525-9
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  • 4
    Online Resource
    Online Resource
    Berberine Modulates LPA Function to Inhibit the Proliferation and Inflammation of FLS-RA via p38/ERK MAPK Pathway Mediated by LPA 1
    Hindawi Limited ; 2019
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2019 ( 2019-11-03), p. 1-12
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2019 ( 2019-11-03), p. 1-12
    Abstract: Objective . This study aimed to investigate whether berberine exerted anti-inflammatory and antiproliferative effects on the fibroblast-like synoviocytes of rheumatoid arthritis (FLS-RA) through regulating the lysophosphatidic acid (LPA) function. Methods . Firstly, the expression levels of LPA and lysophosphatidic acid receptor 1 (LPA 1 ) in RA patients, osteoarthritis (OA) patients, and healthy controls were detected. Moreover, molecular docking was employed to characterize the binding sites of berberine in the predicted protein targets. Later, FLS-RA were stimulated using berberine, LPA, and the specific inhibitor (Ki16425) of LPA 1, thereafter, the effects on the proliferation, apoptosis, the release of inflammatory mediators of FLS-RA, and the MAPK pathway were observed. Results . Compared with healthy controls ( n  = 25), the plasma LPA level ( n  = 28) and synovial fluid ( n  = 10) were markedly higher in RA patients. LPA 1 was highly expressed in RA patients ( n  = 4) relative to that in OA patients ( n  = 4). Berberine remarkably inhibited the proliferation and the excessive production of IL-6 and TNF- α in FLS-RA, whereas suppressing the expression of K-ras, c-Raf, and p-38/ERK-phosphorylation. In addition, berberine inhibited the LPA-induced p-38/ERK-phosphorylation through binding to LPA 1 . Conclusions . LPA plays a certain role in promoting the proliferation and inflammation of FLS-RA. Berberine potentially modulates LPA function to suppress the proliferation and inflammation of FLS-RA through blocking the p38/ERK MAPK pathway mediated by LPA 1 . These findings suggest that, berberine possesses potential lipid-regulating, antiarthritis, and synovial hyperplasia inhibition activities against RA, which may provide a promising therapeutic target for the clinical drug development for RA patients with dyslipidemia and high CVD risk.
    Type of Medium: Online Resource
    ISSN: 1741-427X , 1741-4288
    URL: Article
    DOI: 10.1155/2019/2580207
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2148302-4
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