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  • Hindawi Limited  (2)
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  • Hindawi Limited  (2)
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  • 1
    Online Resource
    Online Resource
    Hindawi Limited ; 2019
    In:  Journal of Chemistry Vol. 2019 ( 2019-12-05), p. 1-10
    In: Journal of Chemistry, Hindawi Limited, Vol. 2019 ( 2019-12-05), p. 1-10
    Abstract: The effects of extrusion treatment on the structure and properties of adlay (Job’s tears) were investigated. Adlay flour was extruded through a twin-screw extruder with different parameters, including barrel temperature (80–160°C), moisture content (19–27%), and screw speed (170–330 rpm). The results showed that although the expansion index increased with increasing temperature, an increase in moisture content significantly decreased the EI ( p 〈 0.05 ). Extrusion improved the water solubility index and water absorption index of adlay flour ( p 〈 0.05 ). Furthermore, analysis of the gelating properties revealed that the structure and function of adlay flour had radically changed. After extrusion, the viscosity of the adlay flour decreased (peak viscosity decreased by more than 1000 cP), and its fluidity increased. The rheological data were modeled by the Herschel–Bulkley model. X-ray diffraction experiments showed that extrusion contributed to a decrease in relative crystallinity. Scanning electron microscopy revealed that extrusion damaged the basic structure of adlay flour, causing holes and pits on the extrudate surface. Compared to the native adlay flour, the extrusion resulted in significantly changing the pasting, gelating, thermal, rheological, and morphological properties of adlay flour. In conclusion, the extrusion can alter adlay characteristics, but it is necessary to choose appropriate conditions to attain the desired properties.
    Type of Medium: Online Resource
    ISSN: 2090-9063 , 2090-9071
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2393625-3
    detail.hit.zdb_id: 2703077-5
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  • 2
    In: Journal of Immunology Research, Hindawi Limited, Vol. 2018 ( 2018-07-17), p. 1-11
    Abstract: Background . Peroxisome proliferator-activated receptor- (PPAR-) γ plays critical roles in human metabolic disorders and has recently been implicated as a regulator of cellular proliferation and inflammatory responses. Regulatory T cells (Tregs), which express high levels of PPAR- γ protein, have the ability to maintain immune tolerance to self-antigens and regulate immune response to Schistosoma infection. However, mechanisms involved in the resolution of these responses are elusive. Methods . Liver and spleen tissue samples in Schistosoma japonicum -infected mice after administration of pioglitazone (a PPAR- γ agonist) were collected. The hepatic and splenic pathologies were detected by H & E and Masson staining. The percentages of Th1/2 and Treg cells in the liver and spleen of each mouse were determined using flow cytometry. Levels of gene expression of PPAR- γ and Foxp3 in tissues or cells were determined using real-time PCR (RT-PCR). Macrophages were treated with pioglitazone in vitro or cocultured with normal purified CD4 + T cells for detecting Treg cells by flow cytometry. The interactions of PPAR- γ with Foxp3 in CD4 + T cells were detected by coimmunoprecipitation. Results . Administration of pioglitazone resulted in the prevention of the development of hepatic and splenic pathologies. Activation of PPAR- γ by pioglitazone resulted in increased percentages of CD4 + CD25 + Foxp3 + Treg cells and decreased percentages of CD3 + CD4 + IFN- γ + and CD3 + CD4 + IL-4 + cells in the liver and spleen of Schistosoma japonicum -infected mice. In addition, the PPAR- γ agonist can induce Treg cells in vitro directly or by modulating the macrophage’s function indirectly. Furthermore, through interaction with Foxp3 in CD4 + T cells, the PPAR- γ agonist can promote the expression of Foxp3; however, the inhibitor of PPAR- γ weakened the expression of Foxp3 by modifying the coexpression of Foxp3 and PPAR- γ . Conclusions . Our study reveals a previously unrecognized role for PPAR- γ /Foxp3 signaling in regulating the immunopathology that occurs during Schistosoma infection through induction of Treg cells.
    Type of Medium: Online Resource
    ISSN: 2314-8861 , 2314-7156
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2018
    detail.hit.zdb_id: 2817541-4
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