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  • Hindawi Limited  (10)
  • 1
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    Based on UPLC-Q-TOF-MS/MS, Systematic Network Pharmacology, and Molecular Docking to Explore the Potential Mechanism of Fructus Aurantii for Major Depression Disorder
    Hindawi Limited ; 2021
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2021 ( 2021-10-8), p. 1-11
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2021 ( 2021-10-8), p. 1-11
    Abstract: Background. Major Depression Disorder (MDD) is a common mental disease that has become one of the world’s major medical diseases. Currently, the Fructus Aurantii (FA) has been widely used to treat depression. However, the active substance ingredients and potential mechanisms of the shell antidepression have not yet been clarified. Method. First, we used ultraperformance liquid chromatography-quadrupole/time-of-flight tandem mass (UPLC-QTOF-MS/MS) technology to identify the chemical composition of the FA. Then, it is predicted for active ingredients, pharmaceutical disease target screening by DiscoveryStudio 2016 (DS), Metascape, and other databases, PPI network diagram, and FC core pathway. Finally, the system network pharmacology results are verified by molecular contact verification. Results. Forty-six compounds in FA were identified, and twelve active ingredients were determined. Various database information, PPI network analysis of 41 intersections, and 20 core targets including DRD2, MTOR, FASP3, and PIK3P1 were integrated. Finally, the MDD treatment is indicated by molecular docking, and the most relevant potential signal pathway is the PI3K-Akt signaling pathway.
    Type of Medium: Online Resource
    ISSN: 1741-4288 , 1741-427X
    URL: Article
    DOI: 10.1155/2021/6486287
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2148302-4
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  • 2
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    Cost-Effectiveness Analysis Based on Intelligent Electronic Medical Arthroscopy for the Treatment of Varus Knee Osteoarthritis
    Hindawi Limited ; 2021
    In:  Journal of Healthcare Engineering Vol. 2021 ( 2021-5-6), p. 1-11
    Details
    In: Journal of Healthcare Engineering, Hindawi Limited, Vol. 2021 ( 2021-5-6), p. 1-11
    Abstract: The incidence of inverted knee osteoarthritis is slowly increasing, there are technical limitations in the treatment, and the operation is difficult. In this article, we will study the benefits and costs of arthroscopic cleaning treatments based on intelligent electronic medicine. This article focuses on knee osteoarthritis patients in the EL database. There are 12 male patients, accounting for 66.67%, and 6 female patients, accounting for 33.33%. The average body mass index (BMI) of the patients was 28.08, the average time from first knee discomfort to surgery was 28.44 months, and the average time of arthroscopic debridement treatment for patients with VKOH knee osteoarthritis was 143.11 minutes. One case of perioperative complication occurred within 35 days after operation, which was a soleus muscle intermuscular venous thrombosis. After immobilization and enhanced anticoagulation for 1 week, it was stable without risk of shedding. The average postoperative study time was 20.00 months. The electronic medical arthroscopy cleaning treatment plan in this article can greatly improve the quality of life of patients and can check the pathological state in time, with low cost. In the course of treatment, comprehensive treatment costs can be saved by 45%. Arthroscopic clean-up treatment can not only reduce knee pain and other uncomfortable symptoms, restore normal knee joint function, and improve the quality of life of patients, but also correct the unequal length of the lower limbs, thereby avoiding spinal degeneration caused by knee instability. Therefore, it is the first choice for the treatment of advanced knee osteoarthritis in patients with VKOH.
    Type of Medium: Online Resource
    ISSN: 2040-2309 , 2040-2295
    URL: Article
    DOI: 10.1155/2021/5569872
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2545054-2
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  • 3
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    Study on City-Level Optimization of Tourism Industry Spatial Organization Nodes and Organization Mode for Tourist Destinations
    Hindawi Limited ; 2020
    In:  Complexity Vol. 2020 ( 2020-08-17), p. 1-13
    Details
    In: Complexity, Hindawi Limited, Vol. 2020 ( 2020-08-17), p. 1-13
    Abstract: Reasonable spatial organization of the tourism industry can improve the utilization efficiency of regional tourism industry elements. Taking Dalian City in China as an example, this paper collects various types of tourism industry data and introduces GIS network analysis technology into tourism studies to determine the location, scale, and number of tourism nodes in Dalian and optimize the spatial organization nodes and organization models of the tourism industry. This will help ease the pressure on tourism reception in the southern area of Dalian and promote better development and utilization of tourism resources and tourism facilities in the central and northern regions. The results show that (1) when using the “minimizing facility points” model, a total of 17 second-level tourism nodes and 5 first-level tourism nodes are obtained after optimization. The location of these nodes is highly correlated with the level of tourist scenic spots, while tourist scenic spots play a significant role in leading and driving tourism nodes. (2) Using the “maximum coverage” model for optimization, 3138 tourism enterprises are connected with tourism nodes, thus realizing the shortest traffic path between tourism enterprises and tourism nodes, which minimizes the total cost of network services. Compared with suburban areas, enterprises in urban tourism areas are densely distributed, meaning that a smaller service radius of tourism nodes can cover more enterprises. (3) A total of 10 first-level tourism channels and 12 second-level tourism channels are optimized using the “nearest facility” model. The first-level tourism channels are mainly distributed in the central and southern areas of Dalian. These channels connect nodes mainly through national and provincial roads. The second-level tourism channels are mainly distributed in the central and northern areas of Dalian. (4) This study aims to analyze the evolution process of the spatial organization mode of Dalian’s tourism industry and construct a hub-spoke network tourism industry spatial organization mode composed of 17 hubs, 22 spokes, and 22 tourism domains. The analysis and construction are designed according to the optimization results of tourism nodes and tourism channels. The research results enrich the theories and technical means of tourism industry spatial organization and provide references and suggestions for local governments or tourism planning decision-makers; they also provide a scientific basis for the rational allocation of tourism industry elements and promote the rational distribution of tourism industry.
    Type of Medium: Online Resource
    ISSN: 1076-2787 , 1099-0526
    URL: Article
    DOI: 10.1155/2020/2807817
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2004607-8
    SSG: 11
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  • 4
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    Hepatic Polarization Accelerated by Mechanical Compaction Involves HNF4 α Activation
    Hindawi Limited ; 2020
    In:  BioMed Research International Vol. 2020 ( 2020-08-05), p. 1-14
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2020 ( 2020-08-05), p. 1-14
    Abstract: There remain few data about the role of homeostatic compaction in hepatic polarization. A previous study has found that mechanical compaction can accelerate hepatocyte polarization; however, the cellular mechanism underlying the effect is mostly unclear. Hepatocyte nuclear factor 4 alpha (HNF4 α ) is crucial for hepatic polarization in liver morphogenesis. Therefore, we sought to identify any possible involvement of HNF4 α in the process of hepatocyte polarization accelerated by mechanical compaction. We first verified in the nonhepatic cell model HEK-293T, and the hepatic cell model primary hepatocytes that the mechanical compaction on cell aggregates simulated by using transient centrifugation can directly activate the expression of HNF4 α promoters. Moreover, data using primary hepatocytes showed that the HNF4 α expression is positively associated with the levels of compaction force: 2.1-folds higher at the mRNA level and 2.1-folds higher at the protein level for 500 g vs. 0 g. Furthermore, activated HNF4 α expression is associated with the enhanced biliary canalicular formation and the increased production of albumin and urea. Pretreatment with Latrunculin B, an inhibitor of F-actin, and SHE78-7, an inhibitor of E-cadherin, which both interrupt the pathway of mechanical transduction, partially but significantly reduced the HNF4 α expression and production of albumin and urea. In conclusion, HNF4 α can be actively involved in the hepatic polarization in the context of environmental mechanical compaction.
    Type of Medium: Online Resource
    ISSN: 2314-6133 , 2314-6141
    URL: Article
    DOI: 10.1155/2020/8016306
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2698540-8
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  • 5
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    The Prognostic Model Established by the Differential Expression Genes Based on CD8+ T Cells to Evaluate the Prognosis and the Response to Immunotherapy in Osteosarcoma
    Hindawi Limited ; 2023
    In:  Mediators of Inflammation Vol. 2023 ( 2023-2-9), p. 1-11
    Details
    In: Mediators of Inflammation, Hindawi Limited, Vol. 2023 ( 2023-2-9), p. 1-11
    Abstract: Osteosarcoma (OS) is a malignant tumor with an extremely poor prognosis, especially in progressive patients. Immunotherapy based on immune checkpoint inhibitors (ICIs) is considered to be a promising treatment option for OS. Due to tumor heterogeneity, only a minority of patients benefit from immunotherapy. Therefore, it is urgent to explore a model that can accurately assess the response of OS to immunotherapy. In this study, we obtained the single-cell RNA sequencing datasets of OS patients from public databases and defined 34 cell clusters by dimensional reduction and clustering analysis. PTPRC was applied to identify immune cell clusters and nonimmune cell clusters. Next, we performed clustering analysis on the immune cell clusters and obtained 25 immune cell subclusters. Immune cells were labeled with CD8A and CD8B to obtain CD8+ T cell clusters. Meanwhile, we extracted the differentially expressed genes (DEGs) of CD8+ T cell clusters and other immune cell clusters. Furthermore, we constructed a prognostic model (CD8-DEG model) based on the obtained DEGs of CD8+ T cells, and verified the excellent predictive ability of this model for the prognosis of OS. Moreover, we further investigated the value of the CD8-DEG model. The results indicated that the risk score of the CD8-DEG model was an independent risk factor for OS patients. Finally, we revealed that the risk score of the CD8-DEG model correlates with the immune profile of OS and can be used to evaluate the response of OS to immunotherapy. In conclusion, our study revealed the critical role of CD8 cells in OS. The risk score model based on CD8-DEGs can provide guidance for prognosis and immunotherapy of OS.
    Type of Medium: Online Resource
    ISSN: 1466-1861 , 0962-9351
    URL: Article
    DOI: 10.1155/2023/6563609
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2008065-7
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  • 6
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    Huoxiang Zhengqi Oral Liquid Attenuates LPS-Induced Acute Lung Injury by Modulating Short-Chain Fatty Acid Levels and TLR4/NF-κB p65 Pathway
    Hindawi Limited ; 2023
    In:  BioMed Research International Vol. 2023 ( 2023-2-17), p. 1-21
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2023 ( 2023-2-17), p. 1-21
    Abstract: Huoxiang Zhengqi Oral Liquid (HZOL) is a classic Chinese patent medicine used in China for more than 1,000 years in treating gastrointestinal and respiratory diseases. Clinically applied HZOL in early respiratory disease stages can reduce the proportion of lung infection patients that progress to severe acute lung injury (ALI). However, few pharmacological studies evaluated its level of protection against ALI. We explored mechanisms of HZOL against ALI by employing network pharmacology, molecular docking, and rat experiments. Firstly, network pharmacology prediction and published biological evaluation of active ingredients of HZOL suggested that HZOL exerted the protective effect in treating ALI mainly in the areas of regulation of cell adhesion, immune response, and inflammatory response and closely related to the NF-κB pathway. Secondly, molecular docking results demonstrated that imperatorin and isoimperatorin combined well with targets in the NF-κB pathway. Finally, ALI rats induced by lipopolysaccharides (LPS) were used to validate prediction after pretreatment with HZOL for 2 weeks. Results confirmed that lung and colon injury occurred in ALI rats. Furthermore, HZOL exerts anti-inflammatory effects on LPS-induced ALI and gut injury by repairing lung and colon pathology, reducing and alleviating pulmonary edema, inhibiting abnormal enhancement of thymus and spleen index, modulating hematologic indices, and increasing levels of total short-chain fatty acids (SCFAs) in the cecum. Additionally, abnormal accumulation of inflammatory cytokines IL-6, IL-1β, TNF-α, and IFN-γ in serum and bronchoalveolar lavage fluid was significantly reduced after pretreating with HZOL. Furthermore, HZOL downregulated the expression of TLR4, CD14, and MyD88 and phosphorylation of NF-κB p65 in lung tissue. Altogether, HZOL was found to exert an anti-inflammatory effect regulation by increasing levels of SCFAs, inhibiting the accumulation of inflammatory cytokines, and attenuating the activation of the TLR4/NF-κB p65 pathway. Our study provided experimental evidences for the application of HZOL in preventing and treating ALI.
    Type of Medium: Online Resource
    ISSN: 2314-6141 , 2314-6133
    URL: Article
    DOI: 10.1155/2023/6183551
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2698540-8
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  • 7
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    Effect of Freeze-Thaw Cycles on Shear Strength Properties of Loess Reinforced with Lignin Fiber
    Hindawi Limited ; 2022
    In:  Geofluids Vol. 2022 ( 2022-5-3), p. 1-19
    Details
    In: Geofluids, Hindawi Limited, Vol. 2022 ( 2022-5-3), p. 1-19
    Abstract: Freeze-thaw cycles caused by climate change can change the structure and strength of the soil. In seasonally frozen soil areas, the use of improved loess as a filling material must consider the effects of freeze-thaw cycles. With the increasingly severe global environmental problems, the search for suitable new environmental protection improvement materials has become one of the hotspots in soil performance improvement research. The purpose of this paper is to use lignin fiber to improve the engineering performance and freeze-thaw resistance of loess and to reduce the negative impact of engineering construction on the environment of the loess area. Based on a series of triaxial shear tests, the effects of freeze-thaw cycles on the stress-strain relationship, shear strength, and Mohr-Coulomb’s strength parameters of loess reinforced with lignin fiber were analyzed. Combined with the volume change and microstructure characteristics of fiber-reinforced loess before and after the freeze-thaw cycles, the reasons for the effects of the freeze-thaw cycles on the shear strength characteristics of fiber-reinforced loess are discussed. The research results showed that after 15 freeze-thaw cycles, the shear strength of loess reinforced with 1% fiber increased by 0.15%, 2.05%, and 1.35% at 80, 140, and 200 kPa, respectively. The shear strength of the reinforced loess with other fiber contents decreases to different degrees, and the maximum reduction ratio can reach 9.54%. Freeze-thaw cycles changed the variation of shear strength and strength parameters with fiber content. When the fiber content is less than 1%, the shear strength, cohesion, and friction angle of fiber-reinforced loess increase the fastest after freeze-thaw cycles. When the fiber content is 1%, the overall destruction effect of freeze-thaw cycles on fiber-reinforced loess is inhibited, and the soil has the best freeze-thaw resistance.
    Type of Medium: Online Resource
    ISSN: 1468-8123 , 1468-8115
    URL: Article
    DOI: 10.1155/2022/8685553
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2045012-6
    SSG: 13
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  • 8
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    Characterization of Metabolites of Leonurine (SCM-198) in Rats after Oral Administration by Liquid Chromatography/Tandem Mass Spectrometry and NMR Spectrometry
    Hindawi Limited ; 2014
    In:  The Scientific World Journal Vol. 2014 ( 2014), p. 1-11
    Details
    In: The Scientific World Journal, Hindawi Limited, Vol. 2014 ( 2014), p. 1-11
    Abstract: Leonurine, a major bioactive component from Herba Leonuri , shows therapeutic potential for cardiovascular disease and stroke prevention in some preclinical experiments. The aim of this study is to characterize metabolites of leonurine in rats using high performance liquid chromatography coupled with tandem mass spectrometry (HPLC/MS/MS). The chromatographic separation was performed on an Agilent ZORBAX SB-C18 column using a gradient elution with acetonitrile/ammonium acetate buffer (10 mM, pH 4.0) solvent system. An information dependent acquisition (IDA) method was developed for screening and identifying metabolites of leonurine under positive ion mode. Compared with control, the interesting compound in the extracted ion chromatogram (XIC) of the in vivo samples was chosen and further identified by analyzing their retention times, changes in observed mass (Δ m/z ), and spectral patterns of product ion utilizing advanced software tool. For the first time, a total of three metabolites were identified, including two phase II metabolites generated by glucuronidation (M1) and sulfation (M2) and one phase I metabolite formed by O-demethylation (M3). Finally, the lead metabolite M1 was isolated from urine and its structure was characterized as leonurine-10-O- β -D-glucuronide by NMR spectroscopy ( 1 H, 13 C, HMBC, and HSQC).
    Type of Medium: Online Resource
    ISSN: 2356-6140 , 1537-744X
    URL: Article
    DOI: 10.1155/2014/947946
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2014
    detail.hit.zdb_id: 2075968-X
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  • 9
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    Effect of Noni on Memory Impairment Induced by Hydrocortisone in Mice
    Hindawi Limited ; 2022
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2022 ( 2022-9-8), p. 1-8
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2022 ( 2022-9-8), p. 1-8
    Abstract: Background. Oxidative stress and memory impairment have been implicated as common functional brain diseases. Nuclear factor E2-related factor 2 (Nrf2) is highly induced in oxidative stress, indicating that Nrf2 is an emerging target of memory therapy. This study aimed to investigate the effect of noni on brain memory impairment induced by hydrocortisone and its protective mechanism in mice. Methods. Male Kunming mice (n = 8/group) were given hydrocortisone by gastric gavage for 14 consecutive days to establish the memory impairment model, except for those in the control group. On the same day, the corresponding drugs were given by gastric gavage. The changes in ethology were examined. The brains were extracted and subjected to western blot analysis and biochemical analyses to assess the activities of antioxidative stress. Results. The middle- and high-dose noni groups exhibited ameliorated ethology, and the high-dose noni group exhibited increased cerebral protein expression of Nrf2, Kelch-like ECH-associated protein 1 (KEAP1), and haem oxygenase-1 (HO-1) compared to the model group. The arrangement of CA3 vertebral cells in the hippocampus of mice was slightly compact, and hyperchromasia and pyknosis were alleviated. Furthermore, biochemical analyses showed that the activities of enzymes related to oxidative stress in the high-dose noni group were increased. Conclusions. Noni might be a powerful antioxidant that can protect nerve cells and may possess potential benefits for the treatment of memory impairment.
    Type of Medium: Online Resource
    ISSN: 1741-4288 , 1741-427X
    URL: Article
    DOI: 10.1155/2022/2781906
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2148302-4
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  • 10
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    Eif2s3y Promotes the Proliferation of Spermatogonial Stem Cells by Activating ERK Signaling
    Hindawi Limited ; 2021
    In:  Stem Cells International Vol. 2021 ( 2021-1-29), p. 1-18
    Details
    In: Stem Cells International, Hindawi Limited, Vol. 2021 ( 2021-1-29), p. 1-18
    Abstract: The future fertility of males with cancer may be irreversibly compromised by chemotherapy and/or radiotherapy. Spermatogonial stem cell transplantation is believed to be a way to restore fertility in men. However, the survival efficiency of transplanted cells is still low. Eukaryotic translation initiation factor 2 subunit 3 and structural gene Y-linked (Eif2s3y) located on the Y chromosome of male animals is a coding gene of eIF2γ which mainly functions in translation initiation. Recently, the emerging role of Eif2s3y in spermatogenesis has been emphasized in several studies. However, the underlying mechanism is still unclear. In addition, how Eif2s3y functions in large animals remains largely unknown. In this study, we obtained the CDS sequence of the Eif2s3y gene from the testis of dairy goats and found that this gene was highly expressed in the testis and was evolutionarily conserved among different species. Interestingly, overexpression of Eif2s3y promoted the proliferation of spermatogonial stem cells of dairy goats by activating the ERK signaling pathway. In animal experiments, overexpressing Eif2s3y promoted transplanted goat spermatogonial stem cells and produced more colonies after microinjection into the seminiferous tubules of infertile mice. In conclusion, our study highlights an undiscovered role of Eif2s3y in dairy goat reproduction. This finding may provide an important basis for future works regarding male spermatogenic cell restoration and represent a major advance toward surrogate sires becoming a tool for disseminating and regenerating germplasm in all mammals.
    Type of Medium: Online Resource
    ISSN: 1687-9678 , 1687-966X
    URL: Article
    DOI: 10.1155/2021/6668658
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2573856-2
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