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  • Hindawi Limited  (50)
  • 1
    Online Resource
    Online Resource
    Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production
    Hindawi Limited ; 2010
    In:  Journal of Biomedicine and Biotechnology Vol. 2010 ( 2010), p. 1-9
    Details
    In: Journal of Biomedicine and Biotechnology, Hindawi Limited, Vol. 2010 ( 2010), p. 1-9
    Abstract: The objective of this study to design a delivery system resistant to the gastrointestinal environment for oral vaccine against porcine rotavirus. Lactococcus lactis NZ9000 was transformed with segments of vP4 of the porcine rotavirus inserted into the pNZ8112 surface-expression vector, and a recombinant L. lactis expressing VP4 protein was constructed. An approximately 27 kDa VP4 protein was confirmed by SDS-PAGE , Western blot and immunostaining analysis. BALB/c mice were immunized orally with VP4-expression recombinant L. lactis and cellular, mucosal and systemic humoral immune responses were examined. Specific anti-VP4 secretory IgA and IgG were found in feces, ophthalmic and vaginal washes and in serum. The induced antibodies demonstrated neutralizing effects on porcine rotavirus infection on MA104 cells. Our findings suggest that oral immunization with VP4-expressing L. lactis induced both specific local and systemic humoral and cellular immune responses in mice.
    Type of Medium: Online Resource
    ISSN: 1110-7243 , 1110-7251
    URL: Article
    DOI: 10.1155/2010/708460
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2010
    detail.hit.zdb_id: 2698540-8
    detail.hit.zdb_id: 2512507-2
    SSG: 12
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  • 2
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    Morphology and ultrastructure of Dabry’s sturgeon ( Acipenser dabryanus ) spermatozoa using scanning and transmission electron microscopy
    Hindawi Limited ; 2019
    In:  Journal of Applied Ichthyology Vol. 35, No. 1 ( 2019-02), p. 192-201
    Details
    In: Journal of Applied Ichthyology, Hindawi Limited, Vol. 35, No. 1 ( 2019-02), p. 192-201
    Type of Medium: Online Resource
    ISSN: 0175-8659 , 1439-0426
    URL: Issue
    URL: Article
    DOI: 10.1111/jai.2019.35.issue-1
    DOI: 10.1111/jai.13836
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 283875-8
    detail.hit.zdb_id: 2020412-7
    SSG: 21,3
    SSG: 12
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  • 3
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    Breast Ultrasound Image Segmentation Algorithm Using Adaptive Region Growing and Variation Level Sets
    Hindawi Limited ; 2022
    In:  Mathematical Problems in Engineering Vol. 2022 ( 2022-10-3), p. 1-15
    Details
    In: Mathematical Problems in Engineering, Hindawi Limited, Vol. 2022 ( 2022-10-3), p. 1-15
    Abstract: To address the features of strong noise, blurred boundaries, and poor imaging quality in breast ultrasound images, we propose a method for segmenting breast ultrasound images using adaptive region growing and variation level sets. First, this method builds a template layer from the difference between the marked image and the original image. Second, the Otsu algorithm is used to measure the target and background using the maximum class variance method to set the threshold. Finally, through the level set of the pixel neighborhood, the boundary points of the adaptive region growth are specified by the level set of the pixel neighborhood, and it is therefore possible to accurately determine the contour perimeter and area of the lesion region. The results demonstrate that the value of Jaccard and Dice for benign tumors is greater than 0.99. Therefore, the segmentation effect of breast images can be achieved by utilizing a breast ultrasound image segmentation approach that uses adaptive region growth and variation level sets.
    Type of Medium: Online Resource
    ISSN: 1563-5147 , 1024-123X
    URL: Article
    DOI: 10.1155/2022/1752390
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2014442-8
    SSG: 11
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  • 4
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    Online Resource
    Application of the Preoperative Assistant System Based on Machine Learning in Hepatocellular Carcinoma Resection
    Hindawi Limited ; 2021
    In:  Journal of Healthcare Engineering Vol. 2021 ( 2021-9-24), p. 1-6
    Details
    In: Journal of Healthcare Engineering, Hindawi Limited, Vol. 2021 ( 2021-9-24), p. 1-6
    Abstract: To conduct better research in hepatocellular carcinoma resection, this paper used 3D machine learning and logistic regression algorithm to study the preoperative assistance of patients undergoing hepatectomy. In this study, the logistic regression model was analyzed to find the influencing factors for the survival and recurrence of patients. The clinical data of 50 HCC patients who underwent extensive hepatectomy (≥4 segments of the liver) admitted to our hospital from June 2020 to December 2020 were selected to calculate the liver volume, simulated surgical resection volume, residual liver volume, surgical margin, etc. The results showed that the simulated liver volume of 50 patients was 845.2 + 285.5 mL, and the actual liver volume of 50 patients was 826.3 ± 268.1 mL, and there was no significant difference between the two groups (t = 0.425; P   〉  0.05). Compared with the logistic regression model, the machine learning method has a better prediction effect, but the logistic regression model has better interpretability. The analysis of the relationship between the liver tumour and hepatic vessels in practical problems has specific clinical application value for accurately evaluating the volume of liver resection and surgical margin.
    Type of Medium: Online Resource
    ISSN: 2040-2309 , 2040-2295
    URL: Article
    DOI: 10.1155/2021/4757668
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2545054-2
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  • 5
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    Ferroptosis-Specific Inhibitor Ferrostatin-1 Relieves H2O2-Induced Redox Imbalance in Primary Cardiomyocytes through the Nrf2/ARE Pathway
    Hindawi Limited ; 2022
    In:  Disease Markers Vol. 2022 ( 2022-2-22), p. 1-10
    Details
    In: Disease Markers, Hindawi Limited, Vol. 2022 ( 2022-2-22), p. 1-10
    Abstract: Objective. Ischemic heart disease (IHD) has always been the focus of attention of many researchers in cardiovascular disease, and its pathogenesis is also very complicated. Ferroptosis may be involved in the occurrence and development of IHD. Methods. First, primary cardiomyocytes were treated with H2O2 to simulate the IHD in vitro model. After pretreatment with different concentrations of ferrostatin-1, cell survival rate was detected by MTT method, cell apoptosis was detected by TUNEL staining and flow cytometry, and the expression of oxidative stress, ferroptosis, and related molecules of Nrf2/ARE pathway was detected by Western blotting (WB) and quantitative real-time polymerase chain reaction (qRT-PCR). Results. The mortality of primary cardiomyocytes in the H2O2 group was obviously increased. Ferrostatin-1 treatment can effectively inhibit cell death, improve antioxidant enzyme activity, inhibit the expression of ferroptosis-related molecules, and activate Nrf2/ARE pathway expression. Conclusion. Ferroptosis-specific inhibitor ferrostatin-1 relieves H2O2-induced redox imbalance in primary cardiomyocytes through the Nrf2/ARE pathway, inhibits ferroptosis, and thereby slows cardiomyocyte death.
    Type of Medium: Online Resource
    ISSN: 1875-8630 , 0278-0240
    URL: Article
    DOI: 10.1155/2022/4539932
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2033253-1
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  • 6
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    On the Open Problem Related to Rank Equalities for the Sum of Finitely Many Idempotent Matrices and Its Applications
    Hindawi Limited ; 2014
    In:  The Scientific World Journal Vol. 2014 ( 2014), p. 1-7
    Details
    In: The Scientific World Journal, Hindawi Limited, Vol. 2014 ( 2014), p. 1-7
    Abstract: Tian and Styan have shown many rank equalities for the sum of two and three idempotent matrices and pointed out that rank equalities for the sum P 1 + ⋯ + P k with P 1 , … , P k be idempotent ( k 〉 3 ) are still open. In this paper, by using block Gaussian elimination, we obtained rank equalities for the sum of finitely many idempotent matrices and then solved the open problem mentioned above. Extensions to scalar-potent matrices and some related matrices are also included.
    Type of Medium: Online Resource
    ISSN: 2356-6140 , 1537-744X
    URL: Article
    DOI: 10.1155/2014/702413
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2014
    detail.hit.zdb_id: 2075968-X
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  • 7
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    Misfire Fault Diagnosis Method for Diesel Engine Based on MEMD and Dispersion Entropy
    Hindawi Limited ; 2021
    In:  Shock and Vibration Vol. 2021 ( 2021-2-1), p. 1-14
    Details
    In: Shock and Vibration, Hindawi Limited, Vol. 2021 ( 2021-2-1), p. 1-14
    Abstract: As a main source of power, diesel engines are widely used in large mechanical systems. Fire failure is a kind of common fault condition, which seriously affects the power and economy of the diesel engine. Previously, scholars mostly used single-channel signal to diagnose the misfire fault of the diesel engine. However, the single-channel signal has limitations in reflecting the information of fault. A novel fault diagnosis method based on MEMD and dispersion entropy is proposed in this paper. Firstly, the multichannel vibration signal of the diesel engine cylinder head is decomposed by multivariate empirical mode decomposition (MEMD), which obtains the IMF component groups with the same frequency in the same order. Then, the IMF component with a large correlation coefficient with the original signal in each group is selected to reconstruct new signal, and dispersion entropy (DE) of the reconstructed signal is calculated as a fault feature vector. Finally, the fault feature vector is input into the support vector machine (SVM) for misfire fault classification. Compared with the other three methods, the results show that the diagnosis method proposed in this paper can effectively extract the fault features and accurately identify the fault type, which is superior to the comparison method.
    Type of Medium: Online Resource
    ISSN: 1875-9203 , 1070-9622
    URL: Article
    DOI: 10.1155/2021/9213697
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2070162-7
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  • 8
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    Online Resource
    Effect of Metal Chelators on γ -Secretase Indicates That Calcium and Magnesium Ions Facilitate Cleavage of Alzheimer Amyloid Precursor Substrate
    Hindawi Limited ; 2011
    In:  International Journal of Alzheimer's Disease Vol. 2011 ( 2011), p. 1-10
    Details
    In: International Journal of Alzheimer's Disease, Hindawi Limited, Vol. 2011 ( 2011), p. 1-10
    Abstract: Gamma-secretase is involved in the production of A β amyloid peptides. It cleaves the transmembrane domain of the amyloid precursor protein (APP) at alternative sites to produce A β and the APP intracellular domain (AICD). Metal ions play an important role in A β aggregation and metabolism, thus metal chelators and ligands represent potential therapeutic agents for AD treatment. A direct effect of metal chelators on γ -secretase has not yet been investigated. The authors used an in vitro   γ -secretase assay consisting of cleavage of APP C100-3XFLAG by endogenous γ -secretase from rodent brains and human neuroblastoma SH-SY5Y, and detected AICD production by western blotting. Adding metalloprotease inhibitors to the reaction showed that clioquinol, phosphoramidon, and zinc metalloprotease inhibitors had no significant effect on γ -secretase activity. In contrast, phenanthroline, EDTA, and EGTA markedly decreased γ -secretase activity that could be restored by adding back calcium and magnesium ions. Mg 2+ stabilized a 1,000 kDa presenilin 1 complex through blue native gel electrophoresis and size-exclusion chromatography. Data suggest that Ca 2+ and Mg 2+ stabilize γ -secretase and enhance its activity.
    Type of Medium: Online Resource
    ISSN: 2090-0252
    URL: Article
    DOI: 10.4061/2011/950932
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2011
    detail.hit.zdb_id: 2573333-3
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  • 9
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    Early Perihematomal Edema Expansion: Definition, Significance, and Association with Outcomes after Intracerebral Hemorrhage
    Hindawi Limited ; 2021
    In:  Oxidative Medicine and Cellular Longevity Vol. 2021 ( 2021-9-12), p. 1-7
    Details
    In: Oxidative Medicine and Cellular Longevity, Hindawi Limited, Vol. 2021 ( 2021-9-12), p. 1-7
    Abstract: Objective. To investigate the association between early perihematomal edema (PHE) expansion and functional outcome in patients with intracerebral hemorrhage (ICH). Methods. Patients with ICH who underwent initial computed tomography (CT) scans within 6 hours after the onset of symptoms and follow-up CT scans within 24 ± 12 hours were included. Absolute PHE increase was defined as the absolute increase in PHE volume from baseline to 24 hours. A receiver-operating characteristic (ROC) curve was generated to determine the cutoff value for early PHE expansion, which was operationally defined as an absolute increase in PHE volume of 〉 6 mL. The outcome of interest was 3-month poor outcome defined as modified Rankin scale score of ≥4. A multivariable logistic regression procedure was used to assess the association between early PHE expansion and outcome after ICH. Results. In 233 patients with ICH, 89 (38.2%) patients had poor outcome at 3-month follow-up. Early PHE expansion was observed in 56 of 233 (24.0%) patients. Patients with early PHE expansion were more likely to have poor functional outcome than those without (43.8% vs. 11.8%, p 〈 0.001 ). After adjusting for age, admission systolic blood pressure, admission Glasgow Coma Scale score, baseline ICH volume and the presence of intraventricular hemorrhage, and time from onset to CT, early PHE expansion was associated with poor outcome (adjusted odds ratio, 4.25; 95% confidence interval, 1.70–10.60; p = 0.002 ). Conclusions. The early PHE expansion was not uncommon in patients with ICH and was correlated with poor outcome following ICH.
    Type of Medium: Online Resource
    ISSN: 1942-0994 , 1942-0900
    URL: Article
    DOI: 10.1155/2021/6249509
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2455981-7
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  • 10
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    Evaluating the Long-Term Efficacy of Acupuncture Therapy for Subacute Poststroke Aphasia: Study Protocol for a Randomized, Blinded, Controlled, Multicentre Trial
    Hindawi Limited ; 2021
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2021 ( 2021-2-12), p. 1-13
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2021 ( 2021-2-12), p. 1-13
    Abstract: Background. Poststroke aphasia (PSA) is a disabling condition that decreases the quality of life, and the duration of the disease harms the quality of life of PSA patients. Acupuncture has been widely employed for PSA. There is some evidence for the immediate treatment efficacy of acupuncture for PSA; however, long-term results after acupuncture may be poorer. Methods. This is a multicentre, randomized, blinded, nonacupoint (NA) acupuncture controlled, multimodal neuroimaging clinical trial. A total of 48 subjects with subacute PSA will be randomly assigned to an acupoint group or an NA control group. The acupoint group will receive acupuncture with normal needling at DU20, EX-HN1, HT5, GB39, EX-HN12, EX-HN13, and CV23. The NA control group will receive acupuncture in locations not corresponding to acupuncture points as sham acupoints. Both groups will receive identical speech and language therapy thrice a week for four weeks. The primary outcome will be the change in the aphasia quotient (AQ) score measured by the Western Aphasia Battery (WAB) test during the 12th week after randomization. Participants will be blindly assessed at prerandomization (baseline) and 4 weeks, 12 weeks, and 24 weeks after randomization. The secondary outcomes include the Boston Diagnostic Aphasia Examination (BDAE) score, the Disease Prognosis Scale score for ischaemic stroke, etc. Magnetic resonance imaging (MRI) and electroencephalogram (EEG) will also be performed at 4-time intervals as secondary outcomes. All scores and image evaluations will be taken at the same point as the linguistic evaluation. The multilevel evaluation technique will be used to assess the long-term efficacy of acupuncture therapy. MRI scans and EEG will be used to assess acupuncture-related neuroplasticity changes. Discussion. The results from our trial will help to supply evidence for the long-term acupuncture effects for PSA over a long follow-up period. It will provide valuable information for future studies in the field of PSA treatment. The trial was registered at the Chinese Clinical Trial Registry on 16 March 2020 (ChiCTR2000030879).
    Type of Medium: Online Resource
    ISSN: 1741-4288 , 1741-427X
    URL: Article
    DOI: 10.1155/2021/8880590
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2148302-4
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