In:
Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2019 ( 2019-05-26), p. 1-8
Abstract:
Chelidonium majus L. (family Papaveraceae), commonly known as greater celandine or tetterwort, has been reported to have antibacterial and anticancer effects and chelidonine is known as a functional metabolite extracted from C. majus . In this study, we observed the cytotoxicity of the alkaloid, chelidonine, and investigated its functional mechanism in T98G glioblastoma cell line. Chelidonine induced apoptosis in a dose-dependent manner, which was accompanied by decreased antiapoptotic protein Mcl-1. Caspase-3 and -9 were activated by treatment with chelidonine, but chelidonine-mediated apoptosis was only partially inhibited by a pan-caspase inhibitor. Chelidonine also induced the translocation of AIF into the nucleus; therefore, it is likely that chelidonine induces T98G cell death through both caspase-dependent and caspase-independent apoptosis pathways. Chelidonine also induced G 2 / M arrest by inducing multipolar spindle assembly, which might also lead to cell death through inhibiting mitosis. Active CDK1, one of factors contributing to the prolongation of G 2 / M phase, induced Mcl-1 degradation increasing mitochondrial instability, which is also an inducer of apoptosis in chelidonine-treated T98G cells. Taken together, these findings indicate that chelidonine induces apoptosis through G 2 / M arrest and Mcl-1 degradation, implying that it may represent a compound for anticancer chemotherapy.
Type of Medium:
Online Resource
ISSN:
1741-427X
,
1741-4288
DOI:
10.1155/2019/6318179
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2019
detail.hit.zdb_id:
2148302-4
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