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  • 1
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    Online Resource
    Study on the Anticancer Activity of Prodigiosin from Variants of Serratia Marcescens QBN VTCC 910026
    Hindawi Limited ; 2022
    In:  BioMed Research International Vol. 2022 ( 2022-4-25), p. 1-11
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2022 ( 2022-4-25), p. 1-11
    Abstract: Prodigiosin (Pg), a secondary metabolism produced by numerous bacterial species, is known as anticancer, antibacterial, antifungal, immunosuppressant, antioxidant, antimalarial properties. Pg has been tested for antitumor activity in many different cancer cell lines but studies in LU-1, KB cell lines, and tumor-bearing mice are still limited. In this study, Serratia marcescens QBN VTCC 910026 strain (GenBank: KX674054.1) was mutated using Ethyl Methanesulfonate (EMS) to increase the production of Pg. One strain known as EMS 5 was capable of increasing prodigiosin biosynthetic yield by 52% when compared to the wild-type strain. Red bacterial pigmented colonies containing Pg were collected from solid media, lysed with acetone, purified with toluene: ethyl acetate at a ratio of 9: 1 (v/v), and then used to evaluate the potential anticancer activity. The purity of Pg was confirmed using a high-performance liquid chromatography (HPLC) method which indicated a 98% rate. Pg chemical formula which was determined using 1H-NMR and 13C-NMR spectroscopy, confirmed as prodigiosin (Pg). Human breast cancer cell lines MCF-7, oropharyngeal cancer KB, and particularly lung cancer LU-1 in vitro were used to test the anticancer activity of purified Pg compound. It showed a strong inhibitory ability in all the cancer cell lines. Furthermore, the isolated Pg had capable of inhibiting tumor growth, the tumor volume decreased by 36.82%, after 28 days. The results indicated that the bacterial prodigiosin from variants Serratia marcescens QBN VTCC 910026 strain is an encouraging fragment suitable for therapeutic applications.
    Type of Medium: Online Resource
    ISSN: 2314-6141 , 2314-6133
    URL: Article
    DOI: 10.1155/2022/4053074
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2698540-8
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  • 2
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    Online Resource
    Cloning, Expression, and Characterization of Xylanase G2 from Aspergillus oryzae VTCC-F187 in Aspergillus niger VTCC-F017
    Hindawi Limited ; 2021
    In:  BioMed Research International Vol. 2021 ( 2021-3-11), p. 1-9
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2021 ( 2021-3-11), p. 1-9
    Abstract: The study focuses on engineering of recombinant Aspergillus niger to produce highly active xylanase. The xylanase G2 encoding gene originating from Aspergillus oryzae VTCC-F187 was cloned, amplified, and inserted into the pAN7.1GluA vector with specific primers possessing BamHI. The recombinant plasmid was introduced into Aspergillus niger VTCC-F017 by chemical methods. The recombinant strain was checked by polymerase chain reaction method and Southern blot. Next, the recombinant protein was expressed and purified by His-tag column. The molecular mass of the purified xylanase G2, as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), was 21 kDa with a specific activity of 1025 IU/mg towards 0.5% (w/v) of birchwood xylan. The optimal temperature and pH were 55°C and pH 6.5, respectively. The enzyme was stable in a temperature ranges 25–40°C and a pH ranges 5–7. The presence of Tween 80 enhanced xylanase activity. Triton X-100, however, had no impact on the function of the enzyme. The xylanase activity was reduced by Tween 20, SDS, and organic solvents. The enzyme was completely inhibited by Hg2+ and partially by Zn2+, Fe2+, and Ag+, while it was slightly stimulated by K+ and EDTA.
    Type of Medium: Online Resource
    ISSN: 2314-6141 , 2314-6133
    URL: Article
    DOI: 10.1155/2021/8840038
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2698540-8
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  • 3
    Online Resource
    Online Resource
    Preparation of Nano-Ag-TiO 2 Composites by Co-60 Gamma Irradiation to Enhance the Photocurrent of Dye-Sensitized Solar Cells
    Hindawi Limited ; 2019
    In:  International Journal of Photoenergy Vol. 2019 ( 2019-06-12), p. 1-8
    Details
    In: International Journal of Photoenergy, Hindawi Limited, Vol. 2019 ( 2019-06-12), p. 1-8
    Abstract: Nano-silver-titanium dioxide (Ag-TiO 2 ) composites were prepared from commercial TiO 2 (P25, Degussa) and silver nitrate (AgNO 3 ) by gamma Co-60 irradiation method with various initial concentrations of AgNO 3 . The nano-AgTiO 2 composites are utilized as the photoanode for dye-sensitized solar cells (DSCs). Under full sunlight illumination (1000 W/m 2 , AM 1.5), the efficiency of DSCs has improved significantly despite the Ag content of below 1%. The DSC—assembled with 0.75 Ag-TiO 2 (0.75% Ag) photoanode—showed that the photocurrent was significantly enhanced from 8.1 mA.cm −2 to 9.5 mA.cm −2 compared to the DSCs using bared TiO 2 photoanode. The unchanged open-circuit voltage resulted in the overall energy conversion efficiency to be increased by 25% from 3.75% to 4.86%. Electrochemical impedance spectroscopy (EIS) analysis showed that the charge transfer resistance is reduced when increasing Ag content, demonstrating that the charge transfer at TiO 2 /dye interface was enhanced in the presence of silver nanoparticles.
    Type of Medium: Online Resource
    ISSN: 1110-662X , 1687-529X
    URL: Article
    DOI: 10.1155/2019/5737952
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2028941-8
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  • 4
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    Online Resource
    Low-Glare Freeform-Surfaced Street Light Luminaire Optimization to Meet Enhanced Road Lighting Standards
    Hindawi Limited ; 2020
    In:  International Journal of Optics Vol. 2020 ( 2020-08-28), p. 1-12
    Details
    In: International Journal of Optics, Hindawi Limited, Vol. 2020 ( 2020-08-28), p. 1-12
    Abstract: To enhance driving safety at night, a new freeform-surface street light luminaire was proposed and evaluated in this study that meets the requirements of the International Commission on Illumination (CIE) M3 class standard for road lighting. The luminaire was designed using simulations to optimize the location of the bulb according to the requirements of the standard. The light source IES file was experimentally obtained for the optimized luminaire prototype with a 150 W ceramic metal halide lamp using an imaging goniophotometer. The trial road lighting simulation results computed by the lighting software DIALux indicated that the proposed luminaire provided an average road surface brightness of 1.1 cd/m 2 (compared to a minimum requirement of 1.0 cd/m 2 ), a brightness uniformity of 0.41 (compared to a minimum requirement of 0.4), a longitudinal brightness uniformity of 0.64 (compared to a minimum requirement of 0.6), and a glare factor of 7.6% (compared to a maximum limit of 15%). The findings of the image goniophotometer tests were then confirmed by the results of a certified mirror goniophotometer test conducted by the Taiwan Accreditation Foundation (TAF). The results of this study can be used to provide improved street lighting designs to meet enhanced international standards.
    Type of Medium: Online Resource
    ISSN: 1687-9384 , 1687-9392
    URL: Article
    DOI: 10.1155/2020/5683264
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2583298-0
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  • 5
    Online Resource
    Online Resource
    Design of Counter Beam Tunnel Lights for CIE 88 : 2004 Regulation in Threshold Zone
    Hindawi Limited ; 2020
    In:  International Journal of Optics Vol. 2020 ( 2020-03-30), p. 1-9
    Details
    In: International Journal of Optics, Hindawi Limited, Vol. 2020 ( 2020-03-30), p. 1-9
    Abstract: To enhance driving safety, a counter beam light is proposed to meet CIE (International Commission on Illumination) specifications for tunnel lighting. The proposed new counter beam light (CBL) acts as a qualified counter beam light to help tunnel road lighting meet the CIE 88 : 2004 regulation standard in the threshold zone in both simulation and in practice. Through appropriate arrangements of the counter beam light and conventional fluorescent lights on the tunnel ceiling, we demonstrate that road tunnel lighting meeting CIE 88 : 2004 standards can be accomplished. Based on LiteStar four-dimensional simulation, the source file created through the measurement of the proposed CBL prototype achieved an average road surface brightness of 121 cd/m 2 , which is greater than the minimum regulation level of 105 cd/m 2 , a brightness uniformity of 0.88 (minimum regulation level of 0.4), longitudinal brightness uniformity of 0.98 (minimum regulation level of 0.6), a glare factor of 4.41% (maximum level of 15%), and a contrast revealing coefficient of 1.08, which is above the 0.6 minimum level in the threshold zone.
    Type of Medium: Online Resource
    ISSN: 1687-9384 , 1687-9392
    URL: Article
    DOI: 10.1155/2020/6145638
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
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  • 6
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    Online Resource
    Preparation and Antifungal Activity Investigation of Oligochitosan-Zn 2+ on Colletotrichum truncatum
    Hindawi Limited ; 2019
    In:  International Journal of Polymer Science Vol. 2019 ( 2019-11-15), p. 1-6
    Details
    In: International Journal of Polymer Science, Hindawi Limited, Vol. 2019 ( 2019-11-15), p. 1-6
    Abstract: This study presents the structural characteristics and the antifungal efficiency of the oligochitosan-Zn 2+ complexes. Oligochitosan with the average molecular weight of ~5 kDa was effectively prepared by gamma-ray irradiation degradation of chitosan in a solution containing H 2 O 2 . The oligochitosan-Zn 2+ complexes with the different molar ratios of -NH 2 /Zn 2+ were prepared by mixing Zn(NO 3 ) 2 into oligochitosan solution. The resultant complexes were characterized by FTIR, XRD, UV-Vis, and ICP-AES. The obtained results demonstrated that Zn 2+ ions were stably bound with oligochitosan molecules through interacting with -OH and -NH 2 groups. The in vitro antifungal effect of oligochitosan-Zn 2+ complexes was assessed against Colletotrichum truncatum , a fungus species causing anthracnose on crops. The antifungal activity was significantly improved as the increase of Zn 2+ content in the complexes. Particularly, the antifungal efficiency of the complexes reached to 75–100% compared to that of about 12% for oligochitosan. Thus, the addition of Zn 2+ into oligochitosan strengthens its performance towards antifungal property and bring forward a new approach for progressing biobased materials for controlling plant diseases.
    Type of Medium: Online Resource
    ISSN: 1687-9422 , 1687-9430
    URL: Article
    DOI: 10.1155/2019/8357381
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2520688-6
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  • 7
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    Online Resource
    Antiproliferative, Anti-Inflammatory Activities, and Molecular Docking Studies of Secondary Metabolites from Macrosolen tricolor
    Hindawi Limited ; 2023
    In:  Journal of Chemistry Vol. 2023 ( 2023-1-2), p. 1-9
    Details
    In: Journal of Chemistry, Hindawi Limited, Vol. 2023 ( 2023-1-2), p. 1-9
    Abstract: In Vietnam, Macrosolen tricolor is used for the treatment of bloating, broken bones, cough, diarrhea, diuretic, rheumatism, and laxative effects. The study aimed to identify the in vitro antiproliferation and anti-inflammation of all fractions and purified compounds from the M. tricolor whole plants, as well as the in silico molecular docking of the potentially cytotoxic compounds. As the results, fractions (MTH.I, MTH.II, MTE.I, and MTE.II) strongly demonstrated antiproliferative properties against three tested cells, MDA-MB-231, RD, and HepG2 (IC50 values ranged from 4.00 ± 0.20 to 70.60 ± 1.44 μg/mL), as well as anti-inflammatory effects (IC50 values ranged from 4.45 ± 0.08 to 23.00 ± 1.18 μg/mL), whereas other fractions meaningfully evidenced selective cytotoxicity and/or anti-inflammation. Therefore, the phytochemical compositions of the active fractions were illuminated, leading to the characterization of eighteen compounds. Compounds (3–5) revealed the most cytotoxic effects towards all examined cells (IC50 values ranged from 6.88 ± 0.12 to 71.64 ± 1.17 μM) and the strongest anti-inflammatory properties (IC50 values of 16.30 ± 0.92, 7.31 ± 0.55, and 9.23 ± 0.60 μM, respectively). Compound 11 showed potential cytotoxicity against MDA-MB-231, RD, and HepG2 cells (IC50 values of 24.42 ± 0.28, 20.60 ± 0.25, and 3.20 ± 0.02 μM, respectively). Furthermore, compounds (4, 5, and 11) interacted with the active site of the apoptosis regulator Bcl-2 protein (PDB ID: 2O2F), were comparable to PAC, and were compatible with their anticancer activity. This project suggests that M. tricolor is a good source of natural antiproliferative and anti-inflammatory agents and contributes to understanding the biological activities of Macrosolen species in traditional Vietnamese medicine.
    Type of Medium: Online Resource
    ISSN: 2090-9071 , 2090-9063
    URL: Article
    DOI: 10.1155/2023/7977620
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2393625-3
    detail.hit.zdb_id: 2703077-5
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  • 8
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    Online Resource
    Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa Stems
    Hindawi Limited ; 2021
    In:  Journal of Chemistry Vol. 2021 ( 2021-4-19), p. 1-8
    Details
    In: Journal of Chemistry, Hindawi Limited, Vol. 2021 ( 2021-4-19), p. 1-8
    Abstract: The study aims to determine the hepatoprotective effect of n-hexane, ethyl acetate, and methanol extracts of the leaves and stems of two Cleome species against carbon tetrachloride- (CCl4-) induced liver toxicity both in vitro using human hepatoma (HepG2) cells and in vivo in rats as well as the hepatoprotective property of all isolated compounds on HepG2. After 72 h of treatment, at the concentrations of 25, 50, and 100 μg/mL, the methanol of C. chelidonii stems (CCSM) ranged from 18.6% to 20.8%, whereas the methanol of C. chelidonii stems (CVSM) increased from 12.3% to 17.2% cell viability. The results show that CCSM and CVSM significantly expressed in vitro hepaprotective activity on HepG2. Therefore, the animals were daily treated with these extracts at the doses of 15, 30, and 45 mg/kg body weight for 5 days, and CCl4 was injected (2 ml/kg body weight, i.p.) on the 2nd and 3rd days. Levels of aspartate aminotransferase (ALT) and alanine aminotransferase (AST) in the blood were measured and compared to the silymarin control. The treatments with CCSM and CVSM (30, and 45 mg/kg) possessed significant hepatoprotection and were comparable with the activity of silymarin. Further, phytochemical studies of these ones were conducted and led to the identification of eight flavonoids: visconoside A (1), visconoside B (2), quercetin 3-O-β-D-glucopyranoside 7-O-α-L-rhamnopyranoside (3), kaempferol 3-O-β-D-glucopyranoside 7-O-α-L-rhamnopyranoside (4), cleomeside A (5), cleomeside B (6), cleomeside C (7), and quercetin-3-O-[β-D-glucopyranosyl-(1⟶2)]-α-L-rhamnopyranoside 7-O-α-L-rhamnopyranoside (8). Two major flavonoids (1 and 4) displayed significant hepatoprotective property (at the concentration of 100 μM, the prevention percentage values were 66.5% and 74.2%, respectively, compared to the quercetin control, with value of 80.3%).
    Type of Medium: Online Resource
    ISSN: 2090-9071 , 2090-9063
    URL: Article
    DOI: 10.1155/2021/5578667
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2393625-3
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  • 9
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    Online Resource
    Zinc/Cobalt-Based Zeolite Imidazolate Frameworks for Simultaneously Degrading Dye and Inhibiting Bacteria
    Hindawi Limited ; 2022
    In:  Journal of Nanomaterials Vol. 2022 ( 2022-9-30), p. 1-11
    Details
    In: Journal of Nanomaterials, Hindawi Limited, Vol. 2022 ( 2022-9-30), p. 1-11
    Abstract: In this work, the zinc/cobalt-based zeolite imidazolate frameworks ((Co/Zn)ZIFs) were synthesized with the solvothermal method. The obtained material was characterized by utilizing scanning electron microscopy (SEM), X-ray diffraction (XRD), UV-Vis diffusive reflectance spectroscopy, and nitrogen adsorption-desorption isotherms. XRD and SEM analyses show that (Co/Zn)ZIFs are composed of nanocrystals with polyhedral shapes of around 50–100 nm and belong to the I-43m space group as those of ZIF-8 and ZIF-67. Optical studies demonstrate a red shift in the absorbance spectrum of (Co/Zn)ZIFs compared with individual components of ZIF-67 or ZIF-8. (Co/Zn)ZIF composite was utilized as photocatalytic material to treat a model aqueous solution containing rhodamine B and bacteria. It was found that (Co/Zn)ZIFs could simultaneously degrade rhodamine B and inhibit bacteria (E. coli and S. aureus). The manufactured composite could catalyze the mineralization of rhodamine B and also exhibited good antibacterial activity against Gram-negative E. coli (93.32% inhibition rate) and Gram-positive S. aureus (90.86% inhibition rate) in the visible-light region within four hours of irradiation. Gram-negative bacteria were more resistant to (Co/Zn)ZIFs than Gram-positive bacteria. (Co/Zn)ZIFs can be used as light-driven catalysts for water and environmental detoxification from organic compounds like dyes and bacteria.
    Type of Medium: Online Resource
    ISSN: 1687-4129 , 1687-4110
    URL: Article
    DOI: 10.1155/2022/8630685
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2229480-6
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  • 10
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    Determination of Pharmaceutical Residues by UPLC-MS/MS Method: Validation and Application on Surface Water and Hospital Wastewater
    Hindawi Limited ; 2021
    In:  Journal of Analytical Methods in Chemistry Vol. 2021 ( 2021-1-8), p. 1-12
    Details
    In: Journal of Analytical Methods in Chemistry, Hindawi Limited, Vol. 2021 ( 2021-1-8), p. 1-12
    Abstract: In this study, an analytical method for the simultaneous determination of 7 major pharmaceutical residues in Vietnam, namely, carbamazepine, ciprofloxacin, ofloxacin, ketoprofen, paracetamol, sulfamethoxazole, and trimethoprim, in surface water and hospital wastewater has been developed. The method includes enrichment and clean-up steps by solid phase extraction using mix-mode cation exchange, followed by identification and quantification using an ultrahigh-performance liquid chromatography and tandem mass spectrometry and employing electrospray ionization (UPLC-ESI-MS/MS). Seven target compounds were separated on the reversed phase column and detected in multiple reaction monitoring (MRM) mode within 6 minutes. The present study also optimized the operating parameters of the mass spectrometer to achieve the highest analytical signals for all target compounds. All characteristic parameters of the analytical method were investigated, including linearity range, limit of detection, limit of quantification, precision, and accuracy. The important parameter in UPLC-ESI-MS/MS, matrix effect, was assessed and implemented via preextraction and postextraction spiking experiments. The overall recoveries of all target compounds were in the ranges from 55% to 109% and 56 % to 115% for surface water and hospital wastewater, respectively. Detection limits for surface water and hospital wastewater were 0.005–0.015 µg L−1 and 0.014–0.123 µg L−1, respectively. The sensitivity of the developed method was allowed for determination of target compounds at trace level in environmental water samples. The in-house validation of the developed method was performed by spiking experiment in both the surface water and hospital wastewater matrix. The method was then applied to analyze several surface water and hospital wastewater samples taken from West Lake and some hospitals in Vietnam, where the level of these pharmaceutical product residues was still missed. Sulfamethoxazole was present at a high detection frequency in both surface water (33% of analyzed samples) and hospital wastewater (81% of analyzed samples) samples.
    Type of Medium: Online Resource
    ISSN: 2090-8873 , 2090-8865
    URL: Article
    DOI: 10.1155/2021/6628285
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2654178-6
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