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  • Hindawi Limited  (12)
  • 1
    Online Resource
    Online Resource
    Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAP/DAHC Matrix
    Hindawi Limited ; 2010
    In:  Journal of Biomedicine and Biotechnology Vol. 2010 ( 2010), p. 1-12
    Details
    In: Journal of Biomedicine and Biotechnology, Hindawi Limited, Vol. 2010 ( 2010), p. 1-12
    Abstract: Selecting an appropriate matrix solution is one of the most effective means of increasing the ionization efficiency of phosphopeptides in matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). In this study, we systematically assessed matrix combinations of 2, 6-dihydroxyacetophenone (DHAP) and diammonium hydrogen citrate (DAHC), and demonstrated that the low ratio DHAP/DAHC matrix was more effective in enhancing the ionization of phosphopeptides. Low femtomole level of phosphopeptides from the tryptic digests of α -casein and β -casein was readily detected by MALDI-TOF-MS in both positive and negative ion mode without desalination or phosphopeptide enrichment. Compared with the DHB/PA matrix, the optimized DHAP/DAHC matrix yielded superior sample homogeneity and higher phosphopeptide measurement sensitivity, particularly when multiple phosphorylated peptides were assessed. Finally, the DHAP/DAHC matrix was applied to identify phosphorylation sites from α -casein and β -casein and to characterize two phosphorylation sites from the human histone H1 treated with Cyclin-Dependent Kinase-1 (CDK1) by MALDI-TOF/TOF MS.
    Type of Medium: Online Resource
    ISSN: 1110-7243 , 1110-7251
    URL: Article
    DOI: 10.1155/2010/759690
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2010
    detail.hit.zdb_id: 2698540-8
    detail.hit.zdb_id: 2512507-2
    SSG: 12
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  • 2
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    Online Resource
    Inhibition of Endoplasmic Reticulum Stress by 4-Phenyl Butyric Acid Presents Therapeutic Effects on Periodontitis: Experimental Studies In Vitro and in Rats
    Hindawi Limited ; 2021
    In:  Stem Cells International Vol. 2021 ( 2021-3-3), p. 1-10
    Details
    In: Stem Cells International, Hindawi Limited, Vol. 2021 ( 2021-3-3), p. 1-10
    Abstract: This study investigated the probable mechanisms of endoplasmic reticulum (ER) stress involved in periodontitis in vitro and in vivo. We isolated periodontal ligament stem cells from periodontitis patients and healthy controls (P-PDLSCs and H-PDLSCs). To further simulate the periodontal microenvironment in patients, lipopolysaccharide (LPS) was used to treat H-PDLSCs. The results showed that periodontitis-related inflammation gave rise to the upregulated expression levels of ER stress representative genes including GRP78, PERK, ATF4, and CHOP. In contrast, the treatment of 4-phenyl butyric acid (4-PBA) remarkably suppressed ER stress and supported cell viability. The increased secretion of proinflammatory factors like TNF-α, IL-1β, and IL-6 and the activation of NF-κB pathway were also attenuated by 4-PBA treatment. Moreover, 4-PBA treatment restored the impaired osteogenic differentiation ability of PDLSCs, as demonstrated by the upregulated expression levels of Runx2 and OCN as well as the enhanced Alizarin red staining. Local administration of 4-PBA could rescue alveolar bone resorption of LPS-induced periodontitis rats. Thus, our findings suggested ER stress might act as a promising therapeutic target against periodontitis.
    Type of Medium: Online Resource
    ISSN: 1687-9678 , 1687-966X
    URL: Article
    DOI: 10.1155/2021/6618943
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2573856-2
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  • 3
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    Differentiation Model Establishment and Differentiation-Related Protein Screening in Primary Cultured Human Sebocytes
    Hindawi Limited ; 2018
    In:  BioMed Research International Vol. 2018 ( 2018), p. 1-10
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2018 ( 2018), p. 1-10
    Abstract: Sebocyte differentiation is a continuous process, but its potential molecular mechanism remains unclear. We aimed to establish a novel sebocyte differentiation model using human primary sebocytes and to identify the expression profiles of differentiation-associated proteins. Primary human sebocytes were cultured on Sebomed medium supplemented with 2% serum for 7 days. Flow cytometry showed that S phase cells were decreased time-dependently, while G1 and subG1 (apoptosis) phase cells increased under serum starvation. Transmission electron microscopy and Oil Red O staining revealed a gradual increase of intracellular lipid accumulation. Expression of proliferation marker was diminished, while expression of differentiation, apoptosis, and lipogenic markers elevated gradually during 7-day culture. iTRAQ analysis identified 3582 expressed proteins in this differentiation model. Compared with day 0, number of differentially expressed proteins was 132, 54, 321, and 96 at days 1, 3, 5, and 7, respectively. Two overexpressed proteins (S100 calcium binding protein P and ferredoxin reductase) and 2 downexpressed proteins (adenosine deaminase and keratin 10) were further confirmed by Western blot and immunohistochemistry.
    Type of Medium: Online Resource
    ISSN: 2314-6133 , 2314-6141
    URL: Article
    DOI: 10.1155/2018/7174561
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2018
    detail.hit.zdb_id: 2698540-8
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  • 4
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    Pantoprazole (PPZ) Inhibits RANKL-Induced Osteoclast Formation and Function In Vitro and Prevents Lipopolysaccharide- (LPS-) Induced Inflammatory Calvarial Bone Loss In Vivo
    Hindawi Limited ; 2020
    In:  Stem Cells International Vol. 2020 ( 2020-11-28), p. 1-13
    Details
    In: Stem Cells International, Hindawi Limited, Vol. 2020 ( 2020-11-28), p. 1-13
    Abstract: Bone remodeling is a process delicately balanced between osteoclastic bone resorption and osteoblastic bone formation. Osteoclasts (OCs) are multinucleated giant cells formed through the fusion of monocytic precursors of the hematopoietic stem cells lineage. OCs are the exclusive cells responsible for the resorption and degradation of the mineralized bone matrix. Pantoprazole (PPZ), a proton pump inhibitor (PPI), is commonly prescribed to reduce excess gastric acid production for conditions such as gastroesophageal reflux disease and peptic ulcer disease. Studies have found contradictory effects of PPI therapy on bone metabolism due to the lack of understanding of the exact underlying mechanism. In this study, we found that PPZ inhibits receptor activator of nuclear factor-κB (NF-κB) ligand- (RANKL-) induced osteoclastogenesis from bone marrow monocytic/macrophage (BMMs) precursors and the bone-resorbing activity of mature OCs. Correspondingly, the expression of OC marker genes was also attenuated. At the molecular level, PPZ treatment was associated with reduced activation of the ERK MAPK signaling pathways crucial to OC differentiation. Additionally, the in vivo administration of PPZ protected mice against lipopolysaccharide- (LPS-) induced inflammatory calvarial bone erosion, as a result of the reduced number and activity of OCs on the calvarial bone surface. Although PPI use is associated with increased risk of osteoporosis and bone fractures, our study provides evidence for the direct inhibitory effect of PPZ on OC formation and bone resorption in vitro and in vivo, suggesting a potential therapeutic use of PPZ in the treatment of osteolytic disease with localized bone destruction.
    Type of Medium: Online Resource
    ISSN: 1687-9678 , 1687-966X
    URL: Article
    DOI: 10.1155/2020/8829212
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2573856-2
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  • 5
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    Online Resource
    Establishment and Validation of a Predictive Model for Radiation-Associated Aspiration Pneumonia in Patients with Radiation-Induced Dysphagia after Nasopharyngeal Carcinoma
    Hindawi Limited ; 2022
    In:  Behavioural Neurology Vol. 2022 ( 2022-8-19), p. 1-10
    Details
    In: Behavioural Neurology, Hindawi Limited, Vol. 2022 ( 2022-8-19), p. 1-10
    Abstract: Introduction. Radiotherapy for patients with head and neck cancers raises their risk of aspiration pneumonia-related death. We aimed to develop and validate a model to predict radiation-associated aspiration pneumonia (RAP) among patients with dysphagia after radiotherapy for nasopharyngeal carcinoma (NPC). Materials and Methods. A total of 453 dysphagic patients with NPC were retrospectively recruited from Sun Yat-Sen Memorial Hospital from January 2012 to January 2018. Patients were randomly divided into training cohort ( n = 302 ) and internal validation cohort ( n = 151 ) at a ratio of 2 : 1. The concordance index (C-index) and calibration curve were used to evaluate the accuracy and discriminative ability of this model. Moreover, decision curve analysis was performed to evaluate the net clinical benefit. The results were externally validated in 203 dysphagic patients from the First People’s Hospital of Foshan. Results. Derived from multivariable analysis of the training cohort, four independent factors were introduced to predict RAP, including Kubota water drinking test grades, the maximum radiation dose of lymph node gross tumor volume (Dmax of the GTVnd), neutrophil count, and erythrocyte sedimentation rate (ESR). The nomogram showed favorable calibration and discrimination regarding the training cohort, with a C-index of 0.749 (95% confidence interval (CI), 0.681 to 0.817), which was confirmed by the internal validation cohort (C-index 0.743; 95% CI, 0.669 to 0.818) and the external validation cohort (C-index 0.722; 95% CI, 0.606 to 0.838). Conclusions. Our study established and validated a simple nomogram for RAP among patients with dysphagia after radiotherapy for NPC.
    Type of Medium: Online Resource
    ISSN: 1875-8584 , 0953-4180
    URL: Article
    DOI: 10.1155/2022/6307804
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2035544-0
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  • 6
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    Two novel recombinant porcine reproductive and respiratory syndrome viruses belong to sublineage 3.5 originating from sublineage 3.2
    Hindawi Limited ; 2019
    In:  Transboundary and Emerging Diseases Vol. 66, No. 6 ( 2019-11), p. 2592-2600
    Details
    In: Transboundary and Emerging Diseases, Hindawi Limited, Vol. 66, No. 6 ( 2019-11), p. 2592-2600
    Type of Medium: Online Resource
    ISSN: 1865-1674 , 1865-1682
    URL: Issue
    URL: Article
    DOI: 10.1111/tbed.v66.6
    DOI: 10.1111/tbed.13320
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2414822-2
    SSG: 22
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  • 7
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    Characterization and Evaluation of the Pathogenicity of a Natural Gene-Deleted Transmissible Gastroenteritis Virus in China
    Hindawi Limited ; 2023
    In:  Transboundary and Emerging Diseases Vol. 2023 ( 2023-3-3), p. 1-13
    Details
    In: Transboundary and Emerging Diseases, Hindawi Limited, Vol. 2023 ( 2023-3-3), p. 1-13
    Abstract: Porcine transmissible gastroenteritis virus is the major pathogen that causes fatal diarrhea in newborn piglets. In this study, a TGEV strain was isolated from the small intestine of diarrhea piglets in Sichuan Province, China, and designated SC2021. The complete genomic sequence of TGEV SC2021 was 28561 bp, revealing a new natural deletion TGEV strain. Based on phylogenetic analyses, TGEV SC2021 belonged to the Miller cluster and was closely related to CN strains. The newborn piglets orally challenged with TGEV SC2021 showed typical watery diarrhea. In addition, macro and micropathological changes in the lungs and intestines were observed. In conclusion, we isolated a new natural deletion virus strain and confirmed that the virus strain has high pathogenicity in newborn piglets. Moreover, macroscopic and microscopic lesions were observed in the lungs and intestines of all TGEV SC2021-infected piglets. In summary, we isolated a new natural deletion TGEV strain and demonstrated that the natural deletion strain showed high pathogenicity in newborn piglets. These data enrich the diversity of TGEV strains and help us to understand the genetic evolution and molecular pathogenesis of TGEV.
    Type of Medium: Online Resource
    ISSN: 1865-1682 , 1865-1674
    URL: Article
    DOI: 10.1155/2023/2652850
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2414822-2
    SSG: 22
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  • 8
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    Trusted QoS Assurance Approach for Composite Service
    Hindawi Limited ; 2021
    In:  Security and Communication Networks Vol. 2021 ( 2021-5-17), p. 1-15
    Details
    In: Security and Communication Networks, Hindawi Limited, Vol. 2021 ( 2021-5-17), p. 1-15
    Abstract: The characteristics of service computing environment, such as service loose coupling, resource heterogeneity, and protocol independence, propose higher demand for the trustworthiness of service computing systems. Trust provisioning for composite services has become a hot research spot worldwide. In this paper, quality of service (QoS) planning techniques are introduced into service composition-oriented QoS provisioning architecture. By QoS planning, the overall QoS requirement of the composite service is decomposed into separate QoS requirement for every constituent atom service, the QoS level of which can subsequently be satisfied through well-designed service entity selection policies. For any single service entity, its QoS level is variable when the deployment environment or the load of service node changes. To mitigate the uncertainty, we put forward QoS preprocessing algorithms to estimate the future QoS levels of service entities with their history execution data. Then, based on the modeling of composite service and QoS planning, we design three algorithms, which include the time preference algorithm, cost/availability (C/A) preference algorithm, and Euclidean distance preference algorithm, to select suitable atomic services meeting the user’s requirements. Finally, by combining genetic algorithm and local-search algorithm, we propose memetic algorithm to meet the QoS requirements of composite service. The effectiveness of the proposed methods by which the QoS requirements can be satisfied up to 90% is verified through experiments.
    Type of Medium: Online Resource
    ISSN: 1939-0122 , 1939-0114
    URL: Article
    DOI: 10.1155/2021/9934138
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2415104-X
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  • 9
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    The Protective Effects of Salidroside from Exhaustive Exercise-Induced Heart Injury by Enhancing the PGC-1 α – NRF1/NRF2 Pathway and Mitochondrial Respiratory Function in Rats
    Hindawi Limited ; 2015
    In:  Oxidative Medicine and Cellular Longevity Vol. 2015 ( 2015), p. 1-9
    Details
    In: Oxidative Medicine and Cellular Longevity, Hindawi Limited, Vol. 2015 ( 2015), p. 1-9
    Abstract: Objective . To test the hypothesis that salidroside (SAL) can protect heart from exhaustive exercise-induced injury by enhancing mitochondrial respiratory function and mitochondrial biogenesis key signaling pathway PGC-1 α –NRF1/NRF2 in rats. Methods . Male Sprague-Dawley rats were divided into 4 groups: sedentary (C), exhaustive exercise (EE), low-dose SAL (LS), and high-dose SAL (HS). After one-time exhaustive swimming exercise, we measured the changes in cardiomyocyte ultrastructure and cardiac marker enzymes and mitochondrial electron transport system (ETS) complexes activities in situ . We also measured mitochondrial biogenesis master regulator PGC-1 α and its downstream transcription factors, NRF1 and NRF2 , expression at gene and protein levels. Results . Compared to C group, the EE group showed marked myocardium ultrastructure injury and decrease of mitochondrial respiratory function P 〈 0.05 and protein levels of PGC-1 α , NRF1 , and NRF2   P 〈 0.05 but a significant increase of PGC-1 α , NRF1 , and NRF2 genes levels P 〈 0.05 ; compared to EE group, SAL ameliorated myocardium injury, increased mitochondrial respiratory function P 〈 0.05 , and elevated both gene and protein levels of PGC-1 α , NRF-1 , and NRF-2 . Conclusion . Salidroside can protect the heart from exhaustive exercise-induced injury. It might act by improving myocardial mitochondrial respiratory function by stimulating the expression of PGC-1 α –NRF1/NRF2 pathway.
    Type of Medium: Online Resource
    ISSN: 1942-0900 , 1942-0994
    URL: Article
    DOI: 10.1155/2015/876825
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2015
    detail.hit.zdb_id: 2455981-7
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  • 10
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    Ligustilide Improves Cognitive Impairment via Regulating the SIRT1/IRE1α/XBP1s/CHOP Pathway in Vascular Dementia Rats
    Hindawi Limited ; 2022
    In:  Oxidative Medicine and Cellular Longevity Vol. 2022 ( 2022-8-16), p. 1-15
    Details
    In: Oxidative Medicine and Cellular Longevity, Hindawi Limited, Vol. 2022 ( 2022-8-16), p. 1-15
    Abstract: Vascular dementia (VaD), the second cause of dementia, is caused by chronic cerebral hypoperfusion, producing progressive damage to cerebral cortex, hippocampus, and white matter. Ligustilide (LIG), one of the main active ingredients of Angelica sinensis, exerts the neuroprotective effect on neurodegenerative diseases. However, the mechanism remains unclear. An in vivo model of bilateral common carotid artery occlusion and in vitro model of oxygen glucose deprivation (OGD) were employed in this study. LIG (20 or 40 mg/kg/day) was intragastrically administered to the VaD rats for four weeks. The results of the Morris water maze test demonstrated that LIG effectively ameliorated learning and memory deficiency in VaD rats. LIG obviously relieved neuronal oxidative stress damage by increasing the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) and decreasing the level of malondialdehyde (MDA) in VaD rats. Nissl staining showed that LIG increased the number of the Nissl body in VaD rats. After LIG administration, the apoptotic-related protein, Bax, was decreased and Bcl-2 was increased in the hippocampus of VaD rats. Moreover, the expressions of sirtuin 1 (SIRT1) and protein disulfide isomerase (PDI) were decreased, binding immunoglobulin protein (BIP) and phospho-inositol-requiring enzyme-1α (P-IRE1α), X-box binding protein 1 (XBP1s), and C/EBP-homologous protein (CHOP) were increased in VaD rats. After LIG treatment, these changes were reversed. The immunofluorescence results further showed that LIG upregulated the expression of SIRT1 and downregulated the expression of P-IRE1α in VaD rats. In addition, in vitro experiment showed that EX-527 (SIRT1 inhibitor) partly abolished the inhibitory effect of LIG on the IRE1α/XBP1s/CHOP pathway. In conclusion, these studies indicated that LIG could improve cognitive impairment by regulating the SIRT1/IRE1α/XBP1s/CHOP pathway in VaD rats.
    Type of Medium: Online Resource
    ISSN: 1942-0994 , 1942-0900
    URL: Article
    DOI: 10.1155/2022/6664990
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2455981-7
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