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  • Hindawi Limited  (92)
  • 1
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    Online Resource
    The Protective Effect of Cx43 Protein-Mediated Phosphocreatine on Myocardial Ischemia/Reperfusion Injury
    Hindawi Limited ; 2021
    In:  Cardiology Research and Practice Vol. 2021 ( 2021-1-22), p. 1-9
    Details
    In: Cardiology Research and Practice, Hindawi Limited, Vol. 2021 ( 2021-1-22), p. 1-9
    Abstract: Objectives. To verify the protective effect of phosphocreatine on myocardium in an ischemic model and the possible mechanism of action. Methods. The model of myocardial ischemia/reperfusion (I/R) was established by the ligation balloon method. 30 SD rats were randomly divided into three groups, n = 10 in each group. Sham operation group: the coronary artery was not blocked and observed for 120 minutes. The ischemia/reperfusion (I/R) group was given ischemia for 30 minutes and ischemia reperfusion for 90 minutes. Phosphocreatine (PCr) group: after 30 minutes of ischemia, the rats were intraperitoneally injected with PCr (200 mg/kg) for 90 minutes. The animal groups of myocardial ischemia/reperfusion model in vitro were the same as those in vivo. The heart was removed by thoracotomy and washed immediately in H-K buffer solution. Then, the heart was installed on the Langendorff instrument. The concentration of PCr perfusion fluid in the PCr group was 10 mmol/L. The changes in coronary blood flow in isolated myocardium were recorded. The heart rate and electrocardiogram were recorded by RM6240BT. At the end of the experiment, myocardial pathological sections and Cx43 immunofluorescence staining were made, and the contents of malondialdehyde (MDA) in myocardial tissue were detected. Results. Phosphocreatinine treatment improved the myocardial ischemia model, performance in electrocardiogram (ECG) changes (ST segment apparent), and histological changes (decrease in necrotic myocardial cells, inflammatory cell infiltration, and a reduction in myocardial edema). At the same time, MDA decreased, while coronary blood flow and Cx43 expression significantly improved. Conclusions. Phosphocreatine can improve the electrocardiogram and restore histologic changes in ischemic myocardium and coronary blood flow. The postulated mechanism is by inhibiting the generation of free oxygen radicals and restoring the expression of Cx43 protein.
    Type of Medium: Online Resource
    ISSN: 2090-0597 , 2090-8016
    URL: Article
    DOI: 10.1155/2021/8838151
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2506187-2
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  • 2
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    Clearance of Free Silica in Rat Lungs by Spraying with Chinese Herbal Kombucha
    Hindawi Limited ; 2013
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2013 ( 2013), p. 1-9
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2013 ( 2013), p. 1-9
    Abstract: The effects of spraying with kombucha and Chinese herbal kombucha were compared with treatments with tetrandrine in a rat silicosis model. Silica dust (50 mg) was injected into the lungs of rats, which were then treated with one of the experimental treatments for a month. The rats were then killed and the effects of the treatments were evaluated by examining the extent and severity of the histopathological lesions in the animals’ lungs, measuring their organ coefficients and lung collagen contents, determining the dry and wet weights of their lungs, and measuring the free silica content of the dried lungs. In addition, lavage was performed on whole lungs taken from selected rats, and the numbers and types of cells in the lavage fluid were counted. The most effective treatment in terms of the ability to reduce lung collagen content and minimize the formation of pulmonary histopathological lesions was tetrandrine treatment, followed by Chinese herbal kombucha and non-Chinese herbal kombucha. However, the lavage fluid cell counts indicated that tetrandrine treatment had severe adverse effects on macrophage viability. This effect was much less pronounced for the kombucha and Chinese herbal kombucha treatments. Moreover, the free silica levels in the lungs of animals treated with Chinese herbal kombucha were significantly lower than those for any other silica-exposed group. These preliminary results indicate that spraying with Chinese herbal kombucha preparations can effectively promote the discharge of silica dust from lung tissues. Chinese herbal kombucha inhalation may thus be a useful new treatment for silicosis and other pneumoconiosis diseases.
    Type of Medium: Online Resource
    ISSN: 1741-427X , 1741-4288
    URL: Article
    DOI: 10.1155/2013/790792
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2013
    detail.hit.zdb_id: 2148302-4
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  • 3
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    A Novel Reference Plasmid for the Qualitative Detection of Genetically Modified Rice in Food and Feed
    Hindawi Limited ; 2015
    In:  BioMed Research International Vol. 2015 ( 2015), p. 1-6
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2015 ( 2015), p. 1-6
    Abstract: Rice is one of the most important food crops in the world. Genetically modified (GM) technology has been used in rice to confer herbicide tolerance and pathogen or insect resistance. China invests heavily in research on GM rice. By the end of 2014, at least 250 transgenic rice lines had been developed in China. To monitor the presence of GM rice in food and feed, we collected information on foreign elements from 250 transgenic rice lines and found 5 elements, including the Agrobacterium tumefaciens nopaline synthase terminator (T-NOS), the cauliflower mosaic virus 35S promoter (CaMV35S), the ubiquitin gene (Ubi), the bar gene, and the hygromycin phosphotransferase gene (Hpt), that are commonly present in GM rice. Therefore, we constructed a novel plasmid (pBJGMM001) that contains fragments of these elements and two endogenous reference genes (the sucrose phosphate synthase gene, SPS, and the phosphoenolpyruvate carboxylase gene, PEPC). pBJGMM001 can serve as a standard for detecting 96% of GM rice lines in China. The primers, amplicons, reaction mixture, and PCR program were developed based on Chinese National Standards. The protocol was validated and determined to be suitable for practical use in monitoring and identifying GM rice.
    Type of Medium: Online Resource
    ISSN: 2314-6133 , 2314-6141
    URL: Article
    DOI: 10.1155/2015/948297
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2015
    detail.hit.zdb_id: 2698540-8
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  • 4
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    Microbial Profiles and Risk Factors of Preexisting Biliary Infection in Patients with Therapeutic Endoscopy
    Hindawi Limited ; 2019
    In:  Gastroenterology Research and Practice Vol. 2019 ( 2019-05-05), p. 1-8
    Details
    In: Gastroenterology Research and Practice, Hindawi Limited, Vol. 2019 ( 2019-05-05), p. 1-8
    Abstract: Background . The bile infection may already exist before the administration of an interventional procedure, despite no clinical manifestations of cholangitis detected. Blood cultures remained negative even in more than half of the febrile cases with cholangitis. Risk factors associated with bacterial growth in bile before the intervention are not well defined. To establish the bacterial profiles isolated from the bile samples and to identify risk factors for bacterial colonization in the bile system. Methods . Individuals who underwent endoscopic retrograde cholangiopancreatography (ERCP) interventions were enrolled. Bile samples were aspirated and were immediately transferred into a sterile tube for storage. Results . Positive bile cultures were detected in 363 (38.0%) of 956 patients, including 322 benign diseases and 41 malignances. Of 363 positive cases, 351 (96.7%) were monoinfection and 12 (3.3%) multi-infection. Escherichia coli were the most common Gram-negative bacteria (210, 56.0%), followed by Klebsiella pneumoniae (45, 12.0%). Enterococcus faecalis represented the most common Gram-positive microorganism (19, 5.07%), while Candida albicans (11, 2.93%) were the dominant fungi. Klebsiella pneumoniae were more frequently detected in malignant diseases ( P = 0.046 ). Age, previous ERCP history or OLT history, and CBD diameter were independent risk factors for positive cultures ( P 〈 0.05 ) while preoperative jaundice drug therapy was the protective factor for bile infection ( P 〈 0.05 ). Conclusion . Monomicrobial infection was dominant among all infections, and Klebsiella pneumoniae strains were more frequently isolated from patients with malignant diseases. To effectively manage patients who are at a high risk for bile infection, a detailed diagnosis and treatment plan for each case should be prepared.
    Type of Medium: Online Resource
    ISSN: 1687-6121 , 1687-630X
    URL: Article
    DOI: 10.1155/2019/1527328
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2435460-0
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  • 5
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    Local Stability of Trench for Diaphragm Walls Passing through Deep Weak Interlayer
    Hindawi Limited ; 2021
    In:  Advances in Materials Science and Engineering Vol. 2021 ( 2021-5-31), p. 1-8
    Details
    In: Advances in Materials Science and Engineering, Hindawi Limited, Vol. 2021 ( 2021-5-31), p. 1-8
    Abstract: When trench construction of the diaphragm wall passes through an ultradeep and weak interlayer, local instability of the trench wall occurs easily. To study the mechanism of the local instability in the trench and by considering the effect of soil arching based on the length of the trench and the angle of internal friction in the weak interlayer, the disturbed area of the trench is defined to be a semiellipse and the local failure stability model of the semiellipse is established. The local stability safety factor of the trench wall is obtained by the limit equilibrium analysis of the model. By conducting parameter sensitivity studies, the results show that the thickness of the overlying strata, the unit weight of the slurry, the angle of internal friction, and the cohesion of the weak interlayer have a great influence on the stability of the trench wall. The semiellipsoid model is used to analyze the stability of the trench wall of the diaphragm wall of a subway station. The calculated results are basically consistent with the field monitoring results. All the work of the paper shows that the model is practical to some extent.
    Type of Medium: Online Resource
    ISSN: 1687-8442 , 1687-8434
    URL: Article
    DOI: 10.1155/2021/9934516
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2501025-6
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  • 6
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    Two novel recombinant porcine reproductive and respiratory syndrome viruses belong to sublineage 3.5 originating from sublineage 3.2
    Hindawi Limited ; 2019
    In:  Transboundary and Emerging Diseases Vol. 66, No. 6 ( 2019-11), p. 2592-2600
    Details
    In: Transboundary and Emerging Diseases, Hindawi Limited, Vol. 66, No. 6 ( 2019-11), p. 2592-2600
    Type of Medium: Online Resource
    ISSN: 1865-1674 , 1865-1682
    URL: Issue
    URL: Article
    DOI: 10.1111/tbed.v66.6
    DOI: 10.1111/tbed.13320
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2414822-2
    SSG: 22
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  • 7
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    Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana
    Hindawi Limited ; 2016
    In:  BioMed Research International Vol. 2016 ( 2016), p. 1-11
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2016 ( 2016), p. 1-11
    Abstract: Background . Water channel proteins, also called aquaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis. The full cDNA and protein sequences of aquaporin in Chorispora bungeana Fisch. & C.A. Mey ( C. bungeana ) are still unknown. Results . In this study, PCR and rapid amplification of cDNA ends approaches were used to clone the full cDNA of LRB7 (GenBank accession number: EU636988) of C. bungeana . Sequence analysis indicated that it was 1235 bp, which had two introns and encoded a protein of 250 amino acids. Structure analysis revealed that the protein had two conserved NPA motifs, one of which is MIP signature sequence (SGxHxNPAVT), six membrane helix regions, and additional membrane-embedded domains. Phylogenetic analysis suggested that the protein was from TIP2 subgroup. Surprisingly, semiquantitative RT-PCR experiment and western blot analysis showed that LRB7 and TIP2 were only detectable in roots, unlike Arabidopsis and Raphanus . Connecting with our previous studies, LRB7 was supported to associate with chilling-tolerance in C. bungeana . Conclusion . This is the first time to characterize the full sequences of LRB7 gene and water channel protein in C. bungeana . Our findings contribute to understanding the water transports in plants under low temperatures.
    Type of Medium: Online Resource
    ISSN: 2314-6133 , 2314-6141
    URL: Article
    DOI: 10.1155/2016/2483258
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2016
    detail.hit.zdb_id: 2698540-8
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  • 8
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    Geographic Distribution of HCV Genotypes and Efficacy of Direct-Acting Antivirals in Chronic HCV-Infected Patients in North and Northeast China: A Real-World Multicenter Study
    Hindawi Limited ; 2022
    In:  Canadian Journal of Gastroenterology and Hepatology Vol. 2022 ( 2022-4-29), p. 1-11
    Details
    In: Canadian Journal of Gastroenterology and Hepatology, Hindawi Limited, Vol. 2022 ( 2022-4-29), p. 1-11
    Abstract: Objective. To assess the geographic distribution of HCV genotypes, effectiveness, and safety of DAA treatment for HCV-infected patients in North and Northeast China. Methods. The geographic distribution of HCV genotypes was analyzed in 2162 patients recruited from April 2018 to February 2021. Sustained virologic response rates at 12 (SVR12) or 24 (SVR24) weeks posttreatment and safety were analyzed in 405 patients who completed DAA treatment according to patient baseline characteristics and treatment. Results. Four genotypes and six subtypes were identified as follows: 1b (1187, 54.90%), 2a (790, 36.54%), 3a/b (134, 6.20%), 6a/n (44, 2.04%), mixed genotypes (2a-6a or 2a-3a) (7, 0.32%). Overall, 99.01% patients achieved SVR12, while 98.43% achieved SVR24. All patients treated with elbasvir/grazoprevir (EBR/GZR), sofosbuvir/velpatasvir ± ribavirin (SOF/VEL ± RBV), and SOF/ledipasvir (LDV) achieved SVR12 or SVR24; 92.86% SVR12 and 95.83% SVR24 were observed in patients using SOF + RBV. SVR12 was higher in noncirrhosis versus compensated cirrhosis patients (100% vs. 97.09%, p = 0.022 ). No severe drug-related adverse event was observed. Conclusions. Genotypes 1b and 2a were dominant subtypes in North and Northeast China. The approved drug regimens EBR/GZR and SOF/LDV for subtype 1b and SOF/VEL for nongenotype 1b are the optimal effective and safety profile.
    Type of Medium: Online Resource
    ISSN: 2291-2797 , 2291-2789
    URL: Article
    DOI: 10.1155/2022/7395506
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2762184-4
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  • 9
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    Reconfigurable Intelligent Surface-Aided Cell-Free Massive MIMO with Low-Resolution ADCs: Secrecy Performance Analysis and Optimization
    Hindawi Limited ; 2022
    In:  Wireless Communications and Mobile Computing Vol. 2022 ( 2022-11-3), p. 1-15
    Details
    In: Wireless Communications and Mobile Computing, Hindawi Limited, Vol. 2022 ( 2022-11-3), p. 1-15
    Abstract: The secure communication in reconfigurable intelligent surface-aided cell-free massive MIMO system is investigated with low-resolution ADCs and with the existence of an active eavesdropper. Specifically, an aggregated channel estimation approach is applied to decrease the overhead required to estimate the channels. Using the available imperfect channel state information (CSI), the conjugate beamforming and random beamforming are applied at the APs and the RIS for downlink data transmission, respectively. The closed-form expression of the achievable secrecy rate is acquired to appraise the achievable secrecy performance using only the channel statistics. With the achievable analytical results, the impacts of the quantization bit of ADCs, channel estimation error, the number of RIS elements, and the number of the APs can be unveiled. Aiming to maximize the minimum achievable rate of all legitimate users subject to security constraints, the power control optimization scheme is first formulated. To tackle this nonconvex property of the proposed optimization problem, a path-following algorithm is then utilized to solve the initial problem with continuous approximations and iterative optimization. Numerical results are presented to verify the achieved results along with availability of the presented power allocation approach.
    Type of Medium: Online Resource
    ISSN: 1530-8677 , 1530-8669
    URL: Article
    DOI: 10.1155/2022/8462350
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2045240-8
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  • 10
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    N‐rich mesoporous carbon supported CoNC and FeNC catalysts derived from o‐phenylenediamine for oxygen reduction reaction
    Hindawi Limited ; 2021
    In:  International Journal of Energy Research Vol. 45, No. 9 ( 2021-07), p. 13531-13544
    Details
    In: International Journal of Energy Research, Hindawi Limited, Vol. 45, No. 9 ( 2021-07), p. 13531-13544
    Type of Medium: Online Resource
    ISSN: 0363-907X , 1099-114X
    URL: Issue
    URL: Article
    DOI: 10.1002/er.v45.9
    DOI: 10.1002/er.6682
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 1480879-1
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