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  • Springer  (90)
  • Wiley-Blackwell  (57)
  • Hindawi  (3)
Document type
Keywords
Publisher
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  • 1
    Electronic Resource
    Electronic Resource
    Micropropagation of Hemidesmus indicus (L.) R. Br. through axillary bud culture (1996)
    Springer
    Plant cell reports 15 (1996), S. 427-430 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: ABSTRACT. A procedure for rapid in vitro propagation of the aromatic and medicinal plant Hemidesmus indicus (L.) R.Br. (Family Asclepiadeceae) from nodal explants is described. The highest shoot multiplication rate of 8.2±0.4 shoots/explant with a 95% frequency was achieved in 5 weeks culture period on Murashige and Skoog medium supplemented with 1.15 μM kinetin and 0.054 μM α-naphthaleneacetic acid. Excised shoots were rooted on the same basal medium supplemented with 1.15 μM kinetin and 7.35 μM indole-3-butyric acid. Shoots derived from subcultures exhibited better rooting response than those from primary cultures. After a hardening phase of 2 weeks, there was a 70% transplantation success in the field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050047
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Direct shoot regeneration from foliar explants of an epiphytic orchid,Acampe praemorsa (Roxb.) Blatter and McCann (1997)
    Springer
    Plant cell reports 16 (1997), S. 583-586 
    Details
    ISSN: 1432-203X
    Keywords: Acampe praemorsa ; Epiphytic orchid ; In vitro plant regeneration ; Thidiazuron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient and reproducible procedure is described for the large-scale propagation of an epiphytic orchid,Acampe praemorsa (Roxb.) B latter and McCann using foliar explants. Shoot buds were induced in basal parts of foliar explants on Murashige and Skoog medium supplemented with N6-benzyladenine (BA), kinetin (Kn) or thidiazuron (TDZ), the latter being most effective at 1.0 mg/1. Shoots formed to a TDZ-containing medium elongated following transfer to a substrate supplemented with 2.0 mg/l 1-naphthaleneacetic acid (NAA) and 0.5 mg/1 BA. NAA at lower concentrations had no beneficial effects on shoot regeneration, whether added to the medium along with BA, Kn or TDZ. However, it promoted shoot elongation and leaf expansion. Higher concentrations of NAA suppressed shoot regeneration. The frequency of shoot regeneration was greatly influenced by the developmental stage and orientation of the leaf. Shoots regenerated from the foliar explants were rooted successfully on MS medium containing 1.0 mg/l indole-3-butyric acid. The plantlets were acclimated and eventually transferred to a garden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01142328
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Micropropagation of Hemidesmus indicus (L.) R. Br. through axillary bud culture (1996)
    Springer
    Plant cell reports 15 (1996), S. 427-430 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure for rapid in vitro propagation of the aromatic and medicinal plant Hemidesmus indicus (L.) R.Br. (Family Asclepiadaceae) from nodal explants is described. The highest shoot multiplication rate of 8.2 ± 0.4 shoots/explant with a 95% frequency was achieved in S weeks culture period on Murashige and Skoog medium supplemented with 1.15 μM kinetin and 0.054 μM α-naphthaleneacetic acid. Excised shoots were rooted on the same basal medium supplemented with 1.15 μM kinetin and 7.35 μM indole-3-butyric acid. Shoots derived from subcultures exhibited better rooting response than those from primary cultures. After a hardening phase of 2 weeks, there was a 70% transplantation success in the field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232069
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plantlet regeneration from cell suspension cultures of palmarosa grass (Cymbopogon martinii) (1997)
    Springer
    Plant cell reports 16 (1997), S. 430-434 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cell suspension culture ; Cymbopogon martinii ; Monocot ; Palmarosa ; Plant regeneration ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cell suspension culture was established from nodal callus of Cymbopogon martinii (Roxb.) Wats in a liquid medium containing Murashige and Skoog (1962) basal salts, vitamins, 100 mg l–1 myo-inositol and 20 g l–1 of sucrose (MS) that was supplemented with 13.6 µM 2,4-dichlorophenoxyacetic acid and 1.15 µM kinetin. An initial inoculum density of 2 × 104 cells ml–1 exhibited optimum cell growth. Calli were obtained 12–15 days after the suspension was plated onto semisolid medium of a similar composition. When calli were transferred to semisolid regeneration medium containing MS + 6.7 µM N 6-benzyl-adenine + 1.15 µM kinetin, somatic embryogenesis and plantlet regeneration occurred after 10–25 days. There was no significant decrease in the regeneration potential of the calli even when the cultures were initiated from 47-week-old cell suspensions. Chromosome counts of cells in suspensions, calli and somatic embryos derived from cultures of different ages revealed the presence of diploids, tetraploids and octaploids. However, the 33 regenerated plants tested were all diploid, indicating that only diploid cells were capable of regeneration in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146788
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plantlet regeneration from cell suspension cultures of palmarosa grass (Cymbopogon martinii) (1997)
    Springer
    Plant cell reports 16 (1997), S. 430-434 
    Details
    ISSN: 1432-203X
    Keywords: Cell suspension culture ; Cymbopogon martinii ; Monocot ; Palmarosa ; Plant regeneration ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cell suspension culture was established from nodal callus ofCymbopogon martinii (Roxb.) Wats in a liquid medium containing Murashige and Skoog (1962) basal salts, vitamins, 100 mg 1−1 myo-inositol and 20 g l−1 of sucrose (MS) that was supplemented with 13.6 μM 2,4-dichlorophenoxyacetic acid and 1.15 μM kinetin. An initial inoculum density of 2 x 104 cells ml−1exhibited optimum cell growth. Calli were obtained 12–15 days after the suspension was plated onto semisolid medium of a similar composition. When calli were transferred to semisolid regeneration medium containing MS + 6.7 μM N 6-benzyl-adenine + 1.15 μM kinetin, somatic embryogenesis and plantlet regeneration occurred after 10–25 days. There was no significant decrease in the regeneration potential of the calli even when the cultures were initiated from 47-week-old cell suspensions. Chromosome counts of cells in suspensions, calli and somatic embryos derived from cultures of different ages revealed the presence of diploids, tetraploids and octaploids. However, the 33 regenerated plants tested were all diploid, indicating that only diploid cells were capable of regeneration in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146788
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    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Direct shoot regeneration from foliar explants of an epiphytic orchid, Acampe praemorsa (Roxb.) Blatter and McCann (1997)
    Springer
    Plant cell reports 16 (1997), S. 583-586 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAcampe praemorsa ; Epiphytic orchid ; In vitro plant regeneration ; Thidiazuron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient and reproducible procedure is described for the large-scale propagation of an epiphytic orchid, Acampe praemorsa (Roxb.) Blatter and McCann using foliar explants. Shoot buds were induced in basal parts of foliar explants on Murashige and Skoog medium supplemented with N6-benzyladenine (BA), kinetin (Kn) or thidiazuron (TDZ), the latter being most effective at 1.0 mg/l. Shoots formed to a TDZ-containing medium elongated following transfer to a substrate supplemented with 2.0 mg/l 1-naphthaleneacetic acid (NAA) and 0.5 mg/l BA. NAA at lower concentrations had no beneficial effects on shoot regeneration, whether added to the medium along with BA, Kn or TDZ. However, it promoted shoot elongation and leaf expansion. Higher concentrations of NAA suppressed shoot regeneration. The frequency of shoot regeneration was greatly influenced by the developmental stage and orientation of the leaf. Shoots regenerated from the foliar explants were rooted successfully on MS medium containing 1.0 mg/l indole-3-butyric acid. The plantlets were acclimated and eventually transferred to a garden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01142328
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    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Efficient plant regeneration from cell suspension-derived callus of East Indian rosewood (Dalbergia latifolia Roxb.) (1998)
    Springer
    Plant cell reports 18 (1998), S. 138-142 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cell suspension culture ; Dalbergia latifolia ; Organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure is outlined for the establishment of a proliferating cell suspension culture of East Indian rosewood (Dalbergia latifolia Roxb.) and efficient plant regeneration from callus derived from such cultures. Callus was induced from hypocotyl segments derived from 1-week-old axenic seedlings on Murashige and Skoog (1962) medium (MS) containing 10.8 μM naphthaleneacetic acid (NAA) and 2.2 μM benzyladenine (BA). Calli were increased by subculturing on MS supplemented with same growth regulators and 10% coconut water (CW). Friable calli were used to initiate cell suspension cultures. Optimum cell proliferation occurred in MS containing 10.8 μM NAA, 2.2 μM BA and 10% CW, using an initial inoculum cell density of 2%. Cell clumps composed of 20–25 cells harvested from suspension cultures at the exponential growth phase readily formed callus within 3 weeks following plating on the semi-solid MS as above. High-frequency shoot-bud differentiation was induced in these calli on MS containing 2.7 μM NAA and 13.3 μM BA. The regeneration frequency declined at higher BA concentrations. The organogenic potential of the cell suspensions was influenced by the age of the culture. Regenerated shoots were rooted on half-strength MS containing 5.7 μM indole-3-acetic acid, 4.9 μM indole-3-butyric acid and 5.3 μM indole-3-propionic acid. The plantlets were acclimatized and established in soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050546
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Enzymic conversion of meso-tartarate to dextro-tartarate in tamarind (1961)
    Springer
    Naturwissenschaften 48 (1961), S. 406-406 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00609104
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Binding ofd-α-tocopherol to rat liver nuclear components (1975)
    Springer
    Cellular and molecular life sciences 31 (1975), S. 1023-1024 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Whend-α-tocopherol is administered i.v. to vitamin E dificient rats, significant amounts of this vitamin are bound to a nucleoprotein complex in hepatic nuclei, and this complex can be solubilized by high concentrations of sodium chloride (0.6M). The bound vitamin in this complex, extractable by ethanol, was found to be identical with authentic α-tocopherol by thin layer chromatography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02326936
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Age-related response of citrate synthase to hydrocortisone in the liver and brain of male rats (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 97-98 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The activity of citrate synthase of the liver and brain of rats shows a gradual increase as a function of age. Adrenalectomy causes no significant change in the activity of citrate synthase in either of these tissues in young, adult or old rats. Administration of hydrocortisone to adrenalectomized rats depresses the activity of this enzyme maximally in the liver and brain of young rats. Administration of actinomycin D tends to normalize, the depressed level of this enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01959123
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    Paper (German National Licenses)
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