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  • Hibiscus Publisher Enterprise  (3)
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  • Hibiscus Publisher Enterprise  (3)
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  • 1
    Online Resource
    Online Resource
    Hibiscus Publisher Enterprise ; 2021
    In:  Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353) Vol. 5, No. 2 ( 2021-12-31), p. 19-27
    In: Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353), Hibiscus Publisher Enterprise, Vol. 5, No. 2 ( 2021-12-31), p. 19-27
    Abstract: More and more people are looking at bioremediation as a cheaper option to physhiochemical techniques for cleaning up pollution from farming, mines, and other chemical industries. Toxic effects of molybdenum on spermatogenesis harm not only humans but also livestock and aquatic life. As a result, efforts are being made to remove it from the ecosystem. A microorganism that can convert soluble molybdenum into colloidal molybdenum blue has been discovered. Phosphate concentrations were optimum between 2.5 and 5, molybdate concentrations between 15 and 20, pH between 6, and temperature between 25 and 34 degrees Celsius for the bacteria to thrive. Absorption spectrum of Mo-blue shows a peak at 865 nm and a shoulder at 700 nm, which indicates that it is in fact reduced phosphomolybdate. Copper, mercury, silver, copper, and chromium are all hazardous heavy metals that hinder the synthesis of Mo-blue. Bacillus sp. strain Zeid 15 is the most likely candidate for the bacterium's identity. As part of our screening, we look for the bacterium's capacity to employ different nitriles and amides as potential electron donors for molybdenum reduction or as substrates for growth. A microplate format was used for the screening. The bacterium was able to use the amides acrylamide and propionamide as sources of electron donor for reduction. Mo-blue production was best supported by acrylamide between 750 and 1250 mg/L, and propionamide between 750 and 1000 mg/L. In addition, these amides including acetamide could support the growth of the bacterium. The modified Gompertz model was utilized to model the growth of this bacterium on amides. The model’s growth parameters obtained were lag periods of 1.372, 1.562 and 1.639 d and maximum specific growth rates of 1.38, 0.95 and 0.734 d-1, for acrylamide, acetamide and propionamide, respectively. The capacity of this bacterium to decontaminate simultaneously amides and molybdenum is a novel characteristic that will be very beneficial in bioremediation.
    Type of Medium: Online Resource
    ISSN: 2716-5353
    Language: Unknown
    Publisher: Hibiscus Publisher Enterprise
    Publication Date: 2021
    Location Call Number Limitation Availability
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  • 2
    Online Resource
    Online Resource
    Hibiscus Publisher Enterprise ; 2021
    In:  Asian Journal of Plant Biology Vol. 3, No. 2 ( 2021-12-31), p. 25-32
    In: Asian Journal of Plant Biology, Hibiscus Publisher Enterprise, Vol. 3, No. 2 ( 2021-12-31), p. 25-32
    Abstract: Physical or chemical procedures could efficiently remove contaminants including pesticides such as carbamates from high concentrations of toxicants. Bioremediation, on the other hand, is frequently a less expensive option in the long term when used at low concentrations. Isolation of multiple toxicants removing microorganisms is the goal of bioremediation. In this paper we report on the molybdenum reduction of the bacterium and its ability to grow on the carbamates carbofuran and carbaryl as carbon sources. Both the carbamates carbofuran and carbaryl cannot support molybdenum reduction when used as the sole carbon sources. Between pH 6.0 and 6.8 and between 30 and 34 oC, the bacterium is most efficient in converting molybdate to Mo-blue. For molybdate reduction, glucose was shown to be the strongest electron donor, with maltose and sucrose coming in second and third, respectively, and d-mannitol and d-adonitol coming in last. Phosphate concentrations of 2.5 to 7.5 mM and molybdate concentrations of 20 to 30 mM are also needed. Identical to that of a decreased phosphomolybdate, the Mo-blue produced by the new Mo-reducing bacteria has an absorption spectrum similar to prior Mo-reducing bacteria. Inhibition of molybdenum reduction was 73.3, 50.1, 50.1 and 20.7 percent, respectively, by mercury, copper, silver and lead at 2 ppm. The bacterium was tentatively identified as Serratia sp. strain Amr-4 after biochemical investigation. This bacterium's ability to detoxify a variety of toxicants is highly sought after, making it a significant bioremediation agent.
    Type of Medium: Online Resource
    ISSN: 2289-5868
    Language: Unknown
    Publisher: Hibiscus Publisher Enterprise
    Publication Date: 2021
    Location Call Number Limitation Availability
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  • 3
    Online Resource
    Online Resource
    Hibiscus Publisher Enterprise ; 2021
    In:  Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353) Vol. 5, No. 1 ( 2021-07-31), p. 12-19
    In: Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353), Hibiscus Publisher Enterprise, Vol. 5, No. 1 ( 2021-07-31), p. 12-19
    Abstract: The accumulation of heavy metals and xenobiotic compounds in soil and aquatic bodies is caused by inappropriate waste disposal, industrial and mining operations, and excessive use of agricultural pesticides. Bioremediation is a more cost-effective way of removing these pollutants than other approaches. A new molybdenum-reducing bacterium with the ability to grow on a variety of polyethylene glycol (PEG)s has been discovered. Based on biochemical test, the bacterium was partially identified as Bacillus sp. strain Neni-8. Mo-blue production required an optimal pH of between 6.3 and 6.5, and between 30 and 37 oC. The carbon source, D-glucose best supported molybdenum reduction. A narrow requirement for phosphate of between 2.5 and 7.5 mM for molybdenum reduction was seen. Sodium molybdate as a substrate for reduction showed maximal reduction between 20 and 30 mM. The molybdenum blue absorption spectrum indicates that its identity was possibly a reduced phosphomolybdate. Several heavy metals such as silver, mercury, copper and chromium inhibited molybdenum reduction by 67.6, 48.7, 36.8 and 17.4 %, respectively. Bacterial growth modelled using the modified Gompertz model with PEG 600 as the best carbon source predicted a maximum growth rate of 15.4 Ln CFU/ml, a maximum specific growth rate of 0.198 h-1 and a lag period of 10.1 h. The novel characteristics of this bacterium are very useful in future bioremediation works.
    Type of Medium: Online Resource
    ISSN: 2716-5353
    Language: Unknown
    Publisher: Hibiscus Publisher Enterprise
    Publication Date: 2021
    Location Call Number Limitation Availability
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