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  • 1
    Electronic Resource
    Electronic Resource
    Light amplification in organic thin films using cascade energy transfer (1997)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 389 (1997), S. 466-469 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] There is currently renewed interest in the development of lasers using solid-state organic and polymeric materials as the gain media. These materials have a number of properties that make them good candidates for such applications — for example, emission bands that are displaced (via a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/38979
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    Paper (German National Licenses)
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  • 2
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    Whole-Genome Restriction Mapping by "Subhaploid"-Based RAD Sequencing: An Efficient and Flexible Approach for Physical Mapping and Genome Scaffolding [Methods, Technology, and Resources] (2017)
    Genetics Society of America (GSA)
    In: Genetics
    Details
    Publication Date: 2017-07-07
    Description: Assembly of complex genomes using short reads remains a major challenge, which usually yields highly fragmented assemblies. Generation of ultradense linkage maps is promising for anchoring such assemblies, but traditional linkage mapping methods are hindered by the infrequency and unevenness of meiotic recombination that limit attainable map resolution. Here we develop a sequencing-based " in vitro " linkage mapping approach (called RadMap), where chromosome breakage and segregation are realized by generating hundreds of "subhaploid" fosmid/bacterial-artificial-chromosome clone pools, and by restriction site-associated DNA sequencing of these clone pools to produce an ultradense whole-genome restriction map to facilitate genome scaffolding. A bootstrap-based minimum spanning tree algorithm is developed for grouping and ordering of genome-wide markers and is implemented in a user-friendly, integrated software package (AMMO). We perform extensive analyses to validate the power and accuracy of our approach in the model plant Arabidopsis thaliana and human. We also demonstrate the utility of RadMap for enhancing the contiguity of a variety of whole-genome shotgun assemblies generated using either short Illumina reads (300 bp) or long PacBio reads (6–14 kb), with up to 15-fold improvement of N50 (~816 kb-3.7 Mb) and high scaffolding accuracy (98.1–98.5%). RadMap outperforms BioNano and Hi-C when input assembly is highly fragmented (contig N50 = 54 kb). RadMap can capture wide-range contiguity information and provide an efficient and flexible tool for high-resolution physical mapping and scaffolding of highly fragmented assemblies.
    Print ISSN: 0016-6731
    Topics: Biology
    Published by Genetics Society of America (GSA)
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