In:
Bioanalysis, Future Science Ltd, Vol. 9, No. 6 ( 2017-03), p. 517-526
Abstract:
Aim: We present a fast magnetic immunoassay, combining magnetic nanoparticles and micromagnets. High magnetic field gradients from micromagnets are used to develop a new approach to the standard ELISA. Materials & methods/results: A proof-of-concept based on colorimetric quantification of antiovalbumin antibody in buffer is performed and compared with an ELISA. After optimization, the magnetic immunoassay exhibits a limit of detection (40 ng/ml) and a dynamic range (40–2500 ng/ml) similar to that of ELISAs developed using same biochemical tools. Conclusion: Micromagnets can be fully integrated in multiwell plates at low cost to allow the efficient capture of immunocomplexes carried by magnetic nanoparticles. The method is generic and permits to perform magnetic ELISA in 30 min.
Type of Medium:
Online Resource
ISSN:
1757-6180
,
1757-6199
DOI:
10.4155/bio-2016-0232
Language:
English
Publisher:
Future Science Ltd
Publication Date:
2017
SSG:
15,3
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