In:
Regenerative Medicine, Future Medicine Ltd, Vol. 12, No. 5 ( 2017-07), p. 503-511
Abstract:
Aim: We tested whether the a simple injection known as the patch assay could reconstitute mature hair follicles by culture-expanded human cells and explored whether the assay could reflect the trichogenicity of cultured cells. Materials & methods: Dissociated culture-expanded fetal or adult scalp dermal cells combined with foreskin keratinocytes were subcutaneously injected into the back skin of immunosuppressive mice to form the patch skin. The patches were collected and characterized and were analyzed for hair formation efficiency. Results: Using culture-expanded human fetal cells, the patch assay can efficiently reconstitute mature hair follicles and the efficiency of hair formation in the patch assay correlates with cell trichogenicity. Conclusion: The patch assay has the potential for testing the trichogenicity of human cells.
Type of Medium:
Online Resource
ISSN:
1746-0751
,
1746-076X
DOI:
10.2217/rme-2017-0017
Language:
English
Publisher:
Future Medicine Ltd
Publication Date:
2017
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