In:
Future Microbiology, Future Medicine Ltd, Vol. 13, No. 1 ( 2018-01), p. 81-95
Abstract:
Aim: To evaluate the effects of population influx of refugees on the prevalence of extended-spectrum β-lactamase-producing Escherichia coli in wastewater networks in Lebanon. Materials & methods: Pulsed-field gel electrophoresis, multilocus sequence typing and antibiotic resistance genes typing were performed. Results: 53.1% of isolates recovered from Al-Qaa refugee camp were positive for the tested resistant determinants compared with 49.1% from river effluents. All isolates carried aac(6)-1b and/or aac(3)-II; none carried armA, rmtB, ant(4′)-Iia, aph(3′)-Ia or carbapenemases. CTX-M-15, TEM-1, OXA-1, CMY-2 and SHV-12 were detected. Single and/or double substitutions were detected in GyrA and ParC. Phylogenetic group B2 and ST6470 were the most prevalent. Pulsed-field gel electrophoresis revealed 19 XbaI patterns and 17 pulsotypes. Conclusion: The introduction of novel resistance patterns into the wastewater network requires effective control.
Type of Medium:
Online Resource
ISSN:
1746-0913
,
1746-0921
DOI:
10.2217/fmb-2017-0093
Language:
English
Publisher:
Future Medicine Ltd
Publication Date:
2018
SSG:
12
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