In:
Frontiers in Bioengineering and Biotechnology, Frontiers Media SA, Vol. 9 ( 2021-7-15)
Abstract:
Rare sugars are regarded as functional biological materials due to their potential applications as low-calorie sweeteners, antioxidants, nucleoside analogs, and immunosuppressants. D -Allose is a rare sugar that has attracted substantial attention in recent years, owing to its pharmaceutical activities, but it is still not widely available. To address this limitation, we continuously produced D -allose from D -allulose using a packed bed reactor with commercial glucose isomerase (Sweetzyme IT). The optimal conditions for D -allose production were determined to be pH 8.0 and 60°C, with 500 g/L D -allulose as a substrate at a dilution rate of 0.24/h. Using these optimum conditions, the commercial glucose isomerase produced an average of 150 g/L D -allose over 20 days, with a productivity of 36 g/L/h and a conversion yield of 30%. This is the first report of the successful continuous production of D -allose from D -allulose by commercial glucose isomerase using a packed bed reactor, which can potentially provide a continuous production system for industrial applications of D -allose.
Type of Medium:
Online Resource
ISSN:
2296-4185
DOI:
10.3389/fbioe.2021.681253
DOI:
10.3389/fbioe.2021.681253.s001
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2719493-0
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