In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 14 ( 2023-11-27)
Abstract:
A comparative analysis of chloroplast (cp) genomes and 45s nuclear ribosomal DNA (nrDNA), and a phylogenomic study of six closely related species (including an overlooked new species) of genus Bupleurum from the western part of Sichuan Province in southwestern China were performed. The six species are similar morphologically and it is difficult to identify them; moreover, their genetic relationships remain unclear. It was found that the cp genomes of the six Bupleurum species were extremely similar, and they were highly homogeneous in terms of cp genome structure, genes and its arrangement. Intergenic spacer rpl 32- trn L, pet A- psb J, trn K- rps 16, and the coding gene ycf 1 were considered highly variable. In phylogenetic trees constructed based on the complete cp genome, protein-coding sequences, nrDNA and ITS sequences, Chinese Bupleurum species all formed two major clades; among these trees, nrDNA tree had the best species resolution; the highly variable regions showed no advantage over other molecular markers. Among the six Bupleurum species, B. malconense, B. sichuanense were close relatives to B. chinense and B. yinchowense , B. chaishoui may also be a consanguinity, while B. microcephalum, B. wenchuanense , and the new species B. pseudochaishoui were closely related. At the end, the new species B. pseudochaishoui Z. Chao sp. nov. was described and illustrated, and a key to the six species was tabulated.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2023.1294670
DOI:
10.3389/fpls.2023.1294670.s001
DOI:
10.3389/fpls.2023.1294670.s002
DOI:
10.3389/fpls.2023.1294670.s003
DOI:
10.3389/fpls.2023.1294670.s004
DOI:
10.3389/fpls.2023.1294670.s005
DOI:
10.3389/fpls.2023.1294670.s006
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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