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  • Frontiers Media SA  (17)
  • 1
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    Methyl Gallate Suppresses the Migration, Invasion, and Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma Cells via the AMPK/NF-κB Signaling Pathway in vitro and in vivo
    Frontiers Media SA ; 2022
    In:  Frontiers in Pharmacology Vol. 13 ( 2022-6-13)
    Details
    In: Frontiers in Pharmacology, Frontiers Media SA, Vol. 13 ( 2022-6-13)
    Abstract: Methyl gallate (MG), a polyphenolic compound found in plants, is widely used in traditional Chinese medicine. MG is known to alleviate several cancer symptoms. However, most studies that have reported the antitumor effects of MG have done so at the cellular level, and the inhibitory effect and therapeutic mechanism of MG in hepatocellular carcinoma (HCC) have not been extensively explored in vivo . We aimed to understand the therapeutic mechanism of MG in HCC in vitro and in vivo . MTT and colony formation assays were used to determine the impact of MG on the proliferation of a human HCC cell line, BEL-7402; wound healing and transwell assays were used to quantify the migration and invasion of HCC cells. Western blotting was used to quantify the expression of the AMPK/NF-κB signaling pathway proteins. In vivo tumor growth was measured in a xenograft tumor nude mouse model treated with MG, and hematoxylin–eosin staining and immunohistochemistry (IHC) were used to visualize the histological changes in the tumor tissue. We found that MG showed anti-proliferative effects both in vitro and in vivo . MG downregulated the protein expression of AMPK, NF-κB, p-NF-κB, and vimentin and upregulated the expression of E-cadherin in a dose-dependent manner. Additionally, MG inhibited the migration and invasion of HCC cells by decreasing MMP9 and MMP2 expression and increasing TIMP-2 expression. These were consistent with the results of IHC in vivo . MG inhibited the proliferation, migration, and invasion of HCC cells. This effect potentially involves the regulation of the AMPK/NF-κB pathway, which in turn impacts epithelial-mesenchymal transition and MMP expression.
    Type of Medium: Online Resource
    ISSN: 1663-9812
    URL: Article
    DOI: 10.3389/fphar.2022.894285
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2587355-6
    SSG: 15,3
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  • 2
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    Data_Sheet_1.PDF
    Frontiers Media SA ; 2021
    In:  Frontiers in Bioinformatics Vol. 1 ( 2021-3-25)
    Details
    In: Frontiers in Bioinformatics, Frontiers Media SA, Vol. 1 ( 2021-3-25)
    Abstract: In proteins, functional centers consist of the key amino acids required to perform molecular functions such as catalysis, ligand-binding, hormone- and gas-sensing. These centers are often embedded within complex multi-domain proteins and can perform important cellular signaling functions that enable fine-tuning of temporal and spatial regulation of signaling molecules and networks. To discover hidden functional centers, we have developed a protocol that consists of the following sequential steps. The first is the assembly of a search motif based on the key amino acids in the functional center followed by querying proteomes of interest with the assembled motif. The second consists of a structural assessment of proteins that harbor the motif. This approach, that relies on the application of computational tools for the analysis of data in public repositories and the biological interpretation of the search results, has to-date uncovered several novel functional centers in complex proteins. Here, we use recent examples to describe a step-by-step guide that details the workflow of this approach and supplement with notes, recommendations and cautions to make this protocol robust and widely applicable for the discovery of hidden functional centers.
    Type of Medium: Online Resource
    ISSN: 2673-7647
    URL: Article
    URL: Article
    DOI: 10.3389/fbinf.2021.652286
    DOI: 10.3389/fbinf.2021.652286.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 3091287-8
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  • 3
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    Table_1.docx
    Frontiers Media SA ; 2020
    In:  Frontiers in Cell and Developmental Biology Vol. 8 ( 2020-8-4)
    Details
    In: Frontiers in Cell and Developmental Biology, Frontiers Media SA, Vol. 8 ( 2020-8-4)
    Type of Medium: Online Resource
    ISSN: 2296-634X
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
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    URL: Article
    URL: Article
    DOI: 10.3389/fcell.2020.00688
    DOI: 10.3389/fcell.2020.00688.s001
    DOI: 10.3389/fcell.2020.00688.s002
    DOI: 10.3389/fcell.2020.00688.s003
    DOI: 10.3389/fcell.2020.00688.s004
    DOI: 10.3389/fcell.2020.00688.s005
    DOI: 10.3389/fcell.2020.00688.s006
    DOI: 10.3389/fcell.2020.00688.s007
    DOI: 10.3389/fcell.2020.00688.s008
    DOI: 10.3389/fcell.2020.00688.s009
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2020
    detail.hit.zdb_id: 2737824-X
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  • 4
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    Image_3.JPEG
    Frontiers Media SA ; 2022
    In:  Frontiers in Human Neuroscience Vol. 16 ( 2022-4-7)
    Details
    In: Frontiers in Human Neuroscience, Frontiers Media SA, Vol. 16 ( 2022-4-7)
    Abstract: Schizophrenia patients with auditory verbal hallucinations (AVHs) are diseased groups of serious psychosis with still unknown etiology. The aim of this research was to identify the neurophysiological correlates of auditory verbal hallucinations. Revealing the neural correlates of auditory hallucination is not merely of great clinical significance, but it is also quite essential to study the pathophysiological correlates of schizophrenia. In this study, 25 Schizophrenia patients with AVHs (AVHs group, 23.2 ± 5.35 years), 52 Schizophrenia patients without AVHs (non-AVHs group, 25.79 ± 5.63 years) and 28 healthy subjects (NC group, 26.14 ± 5.45 years) were enrolled. Dynamic functional connectivity was studied with a sliding-window method and functional connectivity states were then obtained with the k-means clustering algorithm in the three groups. We found that schizophrenia patients with AVHs were characterized by significant decreased static functional connectivity and enhanced variability of dynamic functional connectivity (non-parametric permutation test, Bonferroni correction, p & lt; 0.05). In addition, the AVHs group also demonstrated increased number of brain states, suggesting brain dynamics enhanced in these patients compared with the non-AVHs group. Our findings suggested that there were abnormalities in the connection of brain language regions in auditory verbal hallucinations. It appears that the interruption of connectivity from the language region might be critical to the pathological basis of AVHs.
    Type of Medium: Online Resource
    ISSN: 1662-5161
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fnhum.2022.838181
    DOI: 10.3389/fnhum.2022.838181.s001
    DOI: 10.3389/fnhum.2022.838181.s002
    DOI: 10.3389/fnhum.2022.838181.s003
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2425477-0
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  • 5
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    Study on atomization mechanisms and spray fragmentation characteristics of water and emulsion butachlor
    Frontiers Media SA ; 2023
    In:  Frontiers in Plant Science Vol. 14 ( 2023-10-6)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 14 ( 2023-10-6)
    Abstract: Agricultural chemicals are commonly used to control pests and weeds, but cause pesticide waste problems. Oil-based emulsions are often used as pesticide formulations to improve pesticide utilization. In this study, the spray visualization experiment of the water and oil-based emulsion butachlor is carried out using an ST flat fan nozzle at 0.1–0.5 MPa pressure. The dimensionless method is used to analyze the difference in liquid sheet fragmentation morphology and disintegration process and the influence of different fragmentation methods on droplet size. It is found that the hydrophobic components in pesticide have a significant effect on the morphology and process of atomization fragmentation. When spray liquid is water, the liquid sheet breaks up into liquid ligaments due to the Rayleigh instability, then the ligaments break up into droplets. The side view of a liquid sheet is a large-amplitude wave disturbance. When the spray liquid is the emulsion butachlor, holes are generated on the liquid sheet, then the holes break up into droplets. The fragmentation method of emulsion spray is the perforation mechanism. Compared with water spray, the presence of the pesticide butachlor increases the droplet size and spray angle and improves the uniformity of droplet size distribution but reduces the breakup length. The spray angle shows a power law dependence of the Weber number with a power of 0.17 for all conditions tested here. At 0.3 MPa, DV50 increases 25%, and span decreases from 1.187 to 1.172. This study could provide reference for the addition of agricultural additives, the improvement of spray operation efficiency, and the establishment of spray fragmentation mechanism.
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    DOI: 10.3389/fpls.2023.1265013
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2687947-5
    detail.hit.zdb_id: 2613694-6
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  • 6
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    BTG2 and SerpinB5, a novel gene pair to evaluate the prognosis of lung adenocarcinoma
    Frontiers Media SA ; 2023
    In:  Frontiers in Immunology Vol. 14 ( 2023-3-17)
    Details
    In: Frontiers in Immunology, Frontiers Media SA, Vol. 14 ( 2023-3-17)
    Abstract: Lung adenocarcinoma (LUAD), as the most frequent pathological subtype of non−small cell lung cancer, is often characterized by poor prognosis and low 5-year survival rate. Exploriton of new biomarkers and accurate molecular mechanisms for effectively predicting the prognosis of LUAD patients is still necessary. Presently, BTG2 and SerpinB5, which play important roles in tumors, are studied as a gene pair for the first time with the aim of exploring whether they can be used as potential prognostic markers. Methods Using the bioinformatics method to explore whether BTG2 and SerpinB5 can become independent prognostic factors, and explore their clinical application value and whether they can be used as immunotherapeutic markers. In addition, we also verify the conclusions obtained from external datasets, molecular docking, and SqRT-PCR. Results The results show that compared with normal lung tissue, BTG2 expression level was down-regulated and SerpinB5 was up-regulated in LUAD. Additionally, Kaplan–Meier survival analysis demonstrate that the prognosis of low expression level of BTG2 was poor, and that of high expression level of SerpinB5 was poor, suggesting that both of them can be used as independent prognostic factors. Moreover, the prognosis models of the two genes were constructed respectively in this study, and their prediction effect was verified by external data. Besides, ESTIMATE algorithm reveals the relationship between this gene pair and the immune microenvironment. Furthermore, patients with a high expression level of BTG2 and a low expression level of SerpinB5 have higher immunophenoscore for CTLA-4 and PD-1 inhibitors than patients with a low expression level of BTG2 and a high expression level of SerpinB5, indicating that such patients have a more obvious effect of immunotherapy. Discussion Collectively, all the results demonstrate that BTG2 and SerpinB5 might serve as potential prognostic biomarkers and novel therapeutic targets for LUAD.
    Type of Medium: Online Resource
    ISSN: 1664-3224
    URL: Article
    DOI: 10.3389/fimmu.2023.1098700
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2606827-8
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  • 7
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    DataSheet1.DOCX
    Frontiers Media SA ; 2021
    In:  Frontiers in Pharmacology Vol. 12 ( 2021-8-3)
    Details
    In: Frontiers in Pharmacology, Frontiers Media SA, Vol. 12 ( 2021-8-3)
    Abstract: Background: Ginkgo biloba extract (GBE) and donepezil have been reported to be effective in patients with Alzheimer’s disease (AD). Nonetheless, how these drugs impact spontaneous brain activities and how they consequently improve functional recovery are currently unclear. Objectives: This study was to explore the efficacy of GBE vs. donepezil and their add-on efficacy on functional recovery and the adaption of spontaneous brain activities following pharmacologic treatment in patients with AD. Methods: Patients with AD were enrolled and assigned to the GBE group ( n = 50), the donepezil group ( n = 50), or the combined group ( n = 50). Neuropsychological assessments, including minimum mental state examination (MMSE), Alzheimer’s disease assessment scale-cognition (ADAS-Cog), instrumental activity of daily living (IADL), geriatric depression scale (GDS), neuropsychiatric inventory (NPI), and quality of life in Alzheimer’s disease (QOL-AD), were conducted at baseline, 1 month, 3 months, and 6 months. Resting-state functional magnetic resonance imaging (rs-fMRI) was collected to compare the amplitude of low-frequency fluctuation (ALFF), percent amplitude of fluctuation (PerAF), regional homogeneity (ReHo), and degree centrality (DC) at baseline and 6 months. Results: No major significant differences were detected in all comparisons between groups across all follow-up time points. For intragroup comparison, MMSE and ADAS-Cog scores differed significantly across all follow-ups in three groups. The combined group showed significant improvement of GDS scores between baseline and 6 months ( p = 0.007). The GBE group ( p = 0.044) and donepezil group ( p = 0.012) demonstrated significant improvement of NPI scores between baseline and 6 months. Significant correlations were observed between IADL and ALFF in the right gyrus rectus ( p = 0.03) and in the left superior cerebellum gyrus ( p = 0.01), between GDS and ALFF in the right middle temporal gyrus ( p = 0.01), between NPI and PerAF in the left fusiform gyrus ( p = 0.03), and between MMSE and ReHo in right superior frontal gyrus ( p = 0.04). Conclusion: GBE was comparable with donepezil in the improvement of functional recovery in patients with AD while the combined application of GBE and donepezil seems unnecessary. GBE-mediated improvement of functional recovery was characterized by decreased ALFF values in the right gyrus rectus and decreased PerAF values in the left fusiform gyrus. These featured variations of imaging metrics in specific brain regions may serve as biomarkers in the monitoring of the therapeutic efficacy of GBE.
    Type of Medium: Online Resource
    ISSN: 1663-9812
    URL: Article
    URL: Article
    DOI: 10.3389/fphar.2021.721216
    DOI: 10.3389/fphar.2021.721216.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2587355-6
    SSG: 15,3
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  • 8
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    Development of Halide Perovskite Single Crystal for Radiation Detection Applications
    Frontiers Media SA ; 2020
    In:  Frontiers in Chemistry Vol. 8 ( 2020-4-23)
    Details
    In: Frontiers in Chemistry, Frontiers Media SA, Vol. 8 ( 2020-4-23)
    Type of Medium: Online Resource
    ISSN: 2296-2646
    URL: Article
    DOI: 10.3389/fchem.2020.00268
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2020
    detail.hit.zdb_id: 2711776-5
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  • 9
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    Analysis between preoperative cervical radiographic parameters represented by the K-line tilt and the short-term prognosis of laminoplasty for posterior longitudinal ligament ossification: A retrospective study
    Frontiers Media SA ; 2022
    In:  Frontiers in Surgery Vol. 9 ( 2022-9-22)
    Details
    In: Frontiers in Surgery, Frontiers Media SA, Vol. 9 ( 2022-9-22)
    Abstract: To investigate the relationship between preoperative radiographic parameters and the short-term prognosis of patients with cervical ossification of the posterior longitudinal ligament (OPLL) who underwent laminoplasty (LAMP). Methods A retrospective analysis of Cervical OPLL 50 patients with K-line (+) OPLL with no cervical kyphosis who received LAMP was performed. Based on preoperative neutral position x-ray, the K-line tilt, C2–C7 SVA (sagittal vertical axis), CL (cervical lordosis), T1 slope, and T1 slope-CL were recorded. The JOA (Japanese orthopaedic association scores) score and the cervical kyphosis change were recorded 1 year after surgery. Patients were divided into good and poor prognosis groups according to the median (12.5) of the postoperative JOA score. Results There were differences between the two groups in K-line tilt, C2–C7 SVA, and T1 slope (all p s  & lt; 0.05). There was a strong linear correlation between the three, K-Line tilt, JOA score, and C2–C7 SVA. The degree of influence of K-line tilt, C2–C7 SVA, T1 slope on postoperative JOA score was analyzed using multiple linear regression, and the absolute value of the standardized coefficient Beta were 0.550, 0.319, 0.185, respectively. There was no cervical kyphosis change 1 year after surgery. Conclusion As preoperative cervical parameters, the influence of K-line tilt, C2–C7 SVA, and T1 slope on postoperative JOA score decreases in order. There was a linear relationship between preoperative K-line tilt and postoperative JOA score, implying that patients with cervical OPLL with high K-line tilt were not eligible for LAMP. K-line tilt was not predictive of cervical kyphosis change after LAMP in patients with OPLL at short-term follow-up.
    Type of Medium: Online Resource
    ISSN: 2296-875X
    URL: Article
    DOI: 10.3389/fsurg.2022.950707
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2773823-1
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  • 10
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    Table_4.XLSX
    Frontiers Media SA ; 2023
    In:  Frontiers in Nutrition Vol. 10 ( 2023-8-24)
    Details
    In: Frontiers in Nutrition, Frontiers Media SA, Vol. 10 ( 2023-8-24)
    Abstract: The aim of this study was to assess the global burden of disease from non-communicable chronic diseases (NCD) due to diet low in fruits from 1990 to 2019. Methods Based on data from the Global Burden of Disease (GBD) 2019, the global burden of disease due to diet low in fruits was analyzed for each country or region, disaggregated by disease type, age, sex, and year. The number of deaths and disability-adjusted life years (DALYs), population attributable fraction (PAF), age-standardized mortality rate (ASMR) and age-standardized DALY rate (ASDR) were calculated, and the average annual percentage change (AAPC) was calculated to describe trends in ASMR and ASDR from 1990 to 2019. Results From 1990 to 2019, the number of deaths and DALYs due to diet low in fruits increased by 31.5 and 27.4%, respectively. Among the tertiary diseases, ischemic heart disease, stroke, and diabetes and kidney disease were the top three contributors to the global increase in deaths and DALYs. However, both ASMR and ASDR showed a decreasing trend. The fastest decline in ASMR and ASDR was in stroke, with AAPC of −2.13 (95% CI: −2.22, −2.05, p & lt; 0.05) and −0.56 (95% CI: −0.62, −0.51, p & lt; 0.05), respectively. For GBD regions, high PAF occurred mainly in South Asia, Oceania, and sub-Saharan Africa. Age-specific PAF for stroke and ischemic heart disease death attributable to diet low in fruits was significantly negatively associated with age. Diet low in fruits related ASMR and ASDR showed an M-shaped relationship with the socio-demographic index (SDI), but with an overall decreasing trend. Conclusion The number of deaths and DALYs due to diet low in fruits continues to increase. Therefore, early nutritional interventions should be implemented by the relevant authorities to reduce the burden of diseases caused by diet low in fruits.
    Type of Medium: Online Resource
    ISSN: 2296-861X
    URL: Article
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    DOI: 10.3389/fnut.2023.1202763
    DOI: 10.3389/fnut.2023.1202763.s001
    DOI: 10.3389/fnut.2023.1202763.s002
    DOI: 10.3389/fnut.2023.1202763.s003
    DOI: 10.3389/fnut.2023.1202763.s004
    DOI: 10.3389/fnut.2023.1202763.s005
    DOI: 10.3389/fnut.2023.1202763.s006
    DOI: 10.3389/fnut.2023.1202763.s007
    DOI: 10.3389/fnut.2023.1202763.s008
    DOI: 10.3389/fnut.2023.1202763.s009
    DOI: 10.3389/fnut.2023.1202763.s010
    DOI: 10.3389/fnut.2023.1202763.s011
    DOI: 10.3389/fnut.2023.1202763.s012
    DOI: 10.3389/fnut.2023.1202763.s013
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2776676-7
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