In:
Frontiers in Immunology, Frontiers Media SA, Vol. 12 ( 2021-7-29)
Abstract:
Clear associations have been found between vitamin D deficiency and several autoimmune diseases including multiple sclerosis (MS). However, the benefits of vitamin D supplementation on disease management remain a matter of debate. Objective and Methods Patients with MS ( N =12) and neuromyelitis optica spectrum disorder (NMOSD; N =12) were enrolled along with 15 healthy controls. Changes in lymphocyte subset proportions during stimulation of their peripheral blood mononuclear cells (PBMCs) with the active form of vitamin D, 1,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ), and correlations with serum concentrations of the vitamin D precursor 25-hydroxyvitamin D 3 (serum 25(OH)D 3 ) were explored. The impact of 1,25(OH) 2 D 3 stimulation on the expression of vitamin-D-responsive genes in immune cells was also investigated. Results In both MS and NMOSD, stimulation of PBMCs with 1,25(OH) 2 D 3 followed by steroid suppressed the proliferation of total lymphocytes and T cells. The ratio of CD19 + CD27 + memory B cells (Bmem) to all B cells after stimulation with 1,25(OH) 2 D 3 was negatively correlated with serum 25(OH)D 3 in MS (Spearman’s ρ =–0.594, p =0.042), but positively correlated in NMOSD (Pearson’s r = 0.739, p =0.006). However, there was no relationship between the ratio of Bmem to CD19 + CD24 + CD38 + regulatory B cells and serum 25(OH)D 3 in either MS or NMOSD. In addition, the level of 1,25(OH) 2 D 3 -induced CYP24A1 mRNA expression in PBMCs was significantly and negatively correlated with serum 25(OH)D 3 (for ΔC T , r =0.744, p =0.014) in MS. Conclusion These findings suggest a beneficial impact of stimulation of PBMCs with vitamin D followed by steroid on the T-cell population. The association between patient serum 25(OH)D 3 and the proportion of Bmem under immune-cell stimulation differed between MS and NMOSD. Further investigations are warranted with larger patient populations.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2021.677041
DOI:
10.3389/fimmu.2021.677041.s001
DOI:
10.3389/fimmu.2021.677041.s002
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2606827-8
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