In:
Frontiers in Immunology, Frontiers Media SA, Vol. 13 ( 2022-11-10)
Abstract:
Lympho-epithelial interactions between intestinal T resident memory cells (Trm) and the epithelium have been associated with inflammatory bowel disease (IBD) activity. We developed ex vivo autologous organoid-mucosal T cell cocultures to functionally assess lymphoepithelial interactions in Crohn’s Disease (CD) patients compared to controls. We demonstrate the direct epithelial cell death induced by autologous mucosal T cells in CD patients but not in controls. These findings were positively correlated with T cell infiltration of the organoids. This potential was inhibited by limiting lympho-epithelial interactions through CD103 and NKG2D blocking antibodies. These data directly demonstrate for the first time the direct deleterious effect of mucosal T cells on the epithelium of CD patients. Such ex-vivo models are promising techniques to unravel the pathophysiology of these diseases and the potential mode of action of current and future therapies.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2022.1008456
DOI:
10.3389/fimmu.2022.1008456.s001
DOI:
10.3389/fimmu.2022.1008456.s002
DOI:
10.3389/fimmu.2022.1008456.s003
DOI:
10.3389/fimmu.2022.1008456.s004
DOI:
10.3389/fimmu.2022.1008456.s005
DOI:
10.3389/fimmu.2022.1008456.s006
DOI:
10.3389/fimmu.2022.1008456.s007
DOI:
10.3389/fimmu.2022.1008456.s008
DOI:
10.3389/fimmu.2022.1008456.s009
DOI:
10.3389/fimmu.2022.1008456.s010
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2606827-8
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