In:
Frontiers in Immunology, Frontiers Media SA, Vol. 12 ( 2021-3-30)
Abstract:
Human B-cell differentiation has been extensively investigated on genomic and transcriptomic grounds; however, no studies have accomplished so far detailed analysis of antigen-dependent maturation-associated human B-cell populations from a proteomic perspective. Here, we investigate for the first time the quantitative proteomic profiles of B-cells undergoing antigen-dependent maturation using a label-free LC-MS/MS approach applied on 5 purified B-cell subpopulations (naive, centroblasts, centrocytes, memory and plasma B-cells) from human tonsils (data are available via ProteomeXchange with identifier PXD006191). Our results revealed that the actual differences among these B-cell subpopulations are a combination of expression of a few maturation stage-specific proteins within each B-cell subset and maturation-associated changes in relative protein expression levels, which are related with metabolic regulation. The considerable overlap of the proteome of the 5 studied B-cell subsets strengthens the key role of the regulation of the stoichiometry of molecules associated with metabolic regulation and programming, among other signaling cascades (such as antigen recognition and presentation and cell survival) crucial for the transition between each B-cell maturation stage.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2021.637832
DOI:
10.3389/fimmu.2021.637832.s001
DOI:
10.3389/fimmu.2021.637832.s002
DOI:
10.3389/fimmu.2021.637832.s003
DOI:
10.3389/fimmu.2021.637832.s004
DOI:
10.3389/fimmu.2021.637832.s005
DOI:
10.3389/fimmu.2021.637832.s006
DOI:
10.3389/fimmu.2021.637832.s007
DOI:
10.3389/fimmu.2021.637832.s008
DOI:
10.3389/fimmu.2021.637832.s009
DOI:
10.3389/fimmu.2021.637832.s010
DOI:
10.3389/fimmu.2021.637832.s011
DOI:
10.3389/fimmu.2021.637832.s012
DOI:
10.3389/fimmu.2021.637832.s013
DOI:
10.3389/fimmu.2021.637832.s014
DOI:
10.3389/fimmu.2021.637832.s015
DOI:
10.3389/fimmu.2021.637832.s016
DOI:
10.3389/fimmu.2021.637832.s017
DOI:
10.3389/fimmu.2021.637832.s018
DOI:
10.3389/fimmu.2021.637832.s019
DOI:
10.3389/fimmu.2021.637832.s020
DOI:
10.3389/fimmu.2021.637832.s021
DOI:
10.3389/fimmu.2021.637832.s022
DOI:
10.3389/fimmu.2021.637832.s023
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2606827-8
Permalink