In:
Cryoletters, CryoLetters Limited Liability Partnership, Vol. 44, No. 3 ( 2023-05-01), p. 151-159
Abstract:
BACKGROUND: Semen cryopreservation is a biotechnology used frequently in animal production; however, there are some obstacles, such as those caused by high levels of reactive oxygen species (ROS). Moringa oleifera (MO) is known as a potent source of antioxidants and might
be an important adjuvant . OBJECTIVE: The objective of this study was to determine the effect of different concentrations of MO extract supplementation on goat semen cryopreservation efficiency. MATERIALS AND METHODS: Ejaculates (n=6) from four goat breeders were pooled
and diluted in skimmed milk (SM) or Tris-egg yolk (TEY)-based extenders and supplemented with different concentrations of MO extract (0, 1, 2 and 5 mg/mL). After the freeze-thaw cycle, sperm kinetics and viability were assessed. RESULTS: With the SM extender, straightness, wobble and
plasma membrane integrity were lower than in the control group (ρ 〈 0.05). With the TEY extender, wobble was lower in with 5 mg/mL MO extract than in the control group (ρ 〈 0.05). As regards sperm ultrastructure, evaluated by SEM, the MO extract, regardless of the diluent used,
damaged the membrane of sperm cells in a dose-dependent manner. CONCLUSION: The addition of aqueous extract of MO leaves in both diluents at all concentrations tested affects the parameters of sperm progressivity and damages the plasma membrane in a dose-dependent manner.
Type of Medium:
Online Resource
ISSN:
0143-2044
DOI:
10.54680/fr23310110712
Language:
English
Publisher:
CryoLetters Limited Liability Partnership
Publication Date:
2023
detail.hit.zdb_id:
2139919-0
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