GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Androstenedione as a potential predictor of ovarian response in assisted reproductive technology programs
    Consilium Medicum ; 2020
    In:  Gynecology Vol. 22, No. 1 ( 2020-05-01), p. 38-44
    Details
    In: Gynecology, Consilium Medicum, Vol. 22, No. 1 ( 2020-05-01), p. 38-44
    Abstract: Aim. To assess the possibility of using androstenedione levels in blood serum and follicular fluid to predict ovarian response in assisted reproductive technology programs and to conduct a comparative analysis of the results obtained by 2 methods chemiluminescent immunoassay (CLIA) and high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS). Materials and methods. A prospective study included 55 couples who received in vitro fertilization/intracytoplasmic sperm injection and embryo transfer program therapy. The patients were divided into 3 groups depending on the ovarian response to stimulation: 1st (13 oocytes, n=4), 2nd (49 oocytes, n=27), 3rd (over 10 oocytes, n=24). Androstenedione levels were measured in blood serum obtained on the day of transvaginal ovarian puncture and in follicular fluid samples with CLIA and HPLC-MS/ MS methods at the laboratories of Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology. Results. On the day of transvaginal ovarian puncture, the serum androstenedione levels, which were measured by HPLC-MS/MS, were increasing with an increase of the number of oocytes obtained. The CLIA method revealed a difference in the androstenedione levels between the groups with the number of oocytes obtained of less than 3 and more than 10. Moreover, the androstenedione levels measured by CLIA were significantly different between the patient groups (p0.05). Comparison of serum androstenedione levels measured by CLIA and HPLC-MS/MS, showed high correlations between the values [=0.73 (p0.001)], which makes it possible to use both methods equally, given the existing equipment of the clinical base. Conclusion. Prediction of ovarian response to stimulation is an important step in assisted reproductive technology programs. Measuring androstenedione concentration in blood serum on the day of transvaginal ovarian puncture with highly specific methods (CLIA and HPLC-MS/MS) can be used to predict the degree of ovarian response along with the traditional assessment of the ovarian reserve based on determining anti-Mullerian hormone levels in the early follicular phase of the menstrual cycle.
    Type of Medium: Online Resource
    ISSN: 2079-5831 , 2079-5696
    URL: Article
    DOI: 10.26442/20795696.2020.1.200010
    Language: Unknown
    Publisher: Consilium Medicum
    Publication Date: 2020
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Follicular fluid and assisted reproductive technology programs outcomes (literature review)
    Consilium Medicum ; 2020
    In:  Gynecology Vol. 21, No. 6 ( 2020-04-29), p. 36-40
    Details
    In: Gynecology, Consilium Medicum, Vol. 21, No. 6 ( 2020-04-29), p. 36-40
    Abstract: The review presents data on metabolites in the follicular fluid (FF) from the perspective of reproductive medicine and their use in order to predict outcomes of assisted reproductive technology (ART) programs. It considers various components of this biological medium (hormones, lipids, melatonin, etc.) with an assessment of their predictive value in prognosis of the effectiveness of in vitro fertilization (IVF) programs. The data on experimental directions in this field and the prospects for their use in clinical practice are presented. The article emphasizes that the growing clinical need and the unsolved problem of increasing the effectiveness of ART programs determine the need for further studies of the FF composition. Materials and methods. The review includes data related to this topic from foreign and Russian articles found in PubMed which were published in recent years. Results. Given the established fact of a direct effect of FF composition on growth and maturation of oocytes, and further, on the fertilization process, various FF metabolites are actively investigated as non-invasive markers of quality of oocytes/embryos. The article provides data on the experimental directions in this field and the prospects for their use in clinical practice. However, clinical studies of a relation between various FF metabolites levels and outcomes of IVF programs are contradictory. Conclusion. Owing large economic cost for treatment of infertility with IVF, there is need for expansion and intensification of studies to identify and use reliable predictors in prognosis of ART programs outcomes.
    Type of Medium: Online Resource
    ISSN: 2079-5831 , 2079-5696
    URL: Article
    DOI: 10.26442/20795696.2019.6.190663
    Language: Unknown
    Publisher: Consilium Medicum
    Publication Date: 2020
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Blood bisphenol A concentration in patient with infertility affects in vitro fertilization outcomes
    Consilium Medicum ; 2021
    In:  Gynecology Vol. 23, No. 2 ( 2021-05-27), p. 161-166
    Details
    In: Gynecology, Consilium Medicum, Vol. 23, No. 2 ( 2021-05-27), p. 161-166
    Abstract: Aim. To analyze the relationship between bisphenol A levels in the blood of patients with infertility and in vitro fertilization (IVF) outcomes. Materials and methods. Infertility treatment of 301 married couples was performed using IVF. The level of bisphenol A in the blood of patients was determined by gas chromatography-mass spectrometry. The influence of bisphenol A levels on parameters of early embryogenesis and outcomes of IVF programs was assessed. Results. Bisphenol A was detected in 92.9% (277/298) blood samples of women and in 92.7% (141/154) blood samples of men. Bisphenol A levels in women had a statistically significant positive correlation with the level of bisphenol A in their spouses (r=0.533, p0.0001). No differences were found in the main parameters of early embryogenesis and pregnancy rates in quartile bisphenol A subgroups in women and men. There was a trend towards an increase in the miscarriage rate with an increase in the level of bisphenol A. The threshold level of bisphenol A that increased the miscarriage rate was 0.9 ng/ml for women and 0.4 ng/ml for men. With an increase in the threshold level of bisphenol A in both spouses (men 0.4 ng/ml and women 0.9 ng/ml), the odds ratio for miscarriage after IVF 8.8 (95% confidence interval 0.88113.08). Conclusions. Bisphenol A was found in the majority of infertile patients. A significant positive correlation was noted between the level of bisphenol A in patients and their spouses. An increase in the level of bisphenol A in the blood is associated with an increased risk of early reproductive losses after IVF, which requires further research.
    Type of Medium: Online Resource
    ISSN: 2079-5831 , 2079-5696
    URL: Article
    DOI: 10.26442/20795696.2021.2.200706
    Language: Unknown
    Publisher: Consilium Medicum
    Publication Date: 2021
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Association between vitamin D, 〈i〉VDR〈/i〉 polymorphism and IVF outcomes
    Consilium Medicum ; 2022
    In:  Gynecology Vol. 24, No. 1 ( 2022-01-15), p. 30-34
    Details
    In: Gynecology, Consilium Medicum, Vol. 24, No. 1 ( 2022-01-15), p. 30-34
    Abstract: Aim. To analyze the outcomes of assisted reproductive technology (ART) cycles in patients with different levels of vitamin D. Materials and methods. The study included 100 infertile patients who applied for ART. Vitamin D levels were determined by mass spectrometry. The VDR polymorphism was evaluated using real-time PCR. Results. 50% of patients (n=50) had a sufficient level of vitamin D (group 3), 36% of patients (n=36) had an insufficient level of vitamin D (group 2), 14% of patients (n=14) had a deficiency of vitamin D (group 1). The level of vitamin D was not associated neither with baseline patients characteristics nor with embryological stage of the cycle. The clinical pregnancy rate was lower in the group 1 (vitamin D deficiency), but the difference was not significant. There was a trend to an increase of miscarriage rate in the groups of patients with vitamin D insufficiency and deficiency: 66.7% in group 1, 26.3% in group 2, and 13.6% in group 3. The live birth rate was higher in the groups of patients with normal and insufficient levels of vitamin D compared with the group of vitamin D deficiency: 46.0 and 38.9% compared to 7.1%, p=0.0821. In multivariate analysis clinical pregnancy rate was influenced by vitamin D deficiency and the genetic characteristics of the VDR gene: the presence of the G allele (FOKI-rs2228570). Conclusion. The vitamin D deficiency impairs the effectiveness of ART cycles.
    Type of Medium: Online Resource
    ISSN: 2079-5831 , 2079-5696
    URL: Article
    DOI: 10.26442/20795696.2022.1.201245
    Language: Unknown
    Publisher: Consilium Medicum
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Diagnosis of Ia–Ic stages of serous high-grade ovarian cancerby the lipid profile of blood serum
    Consilium Medicum ; 2021
    In:  Gynecology Vol. 23, No. 4 ( 2021-09-22), p. 335-340
    Details
    In: Gynecology, Consilium Medicum, Vol. 23, No. 4 ( 2021-09-22), p. 335-340
    Abstract: Background. Ovarian cancer is the first fatal malignancy of the female reproductive system. Early detection is associated with better outcomes, but is significantly difficult because of asymptomatic or low-symptomatic course. Aim. To study the possibility of detecting of OC in early stages (IaIc) by the lipid profile of blood serum obtained using high-performance liquid chromatography with mass spectrometric (MS) detection. Materials and methods. An observational "case-control" study was conducted in period November 2019 July 2020 in the Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology. 41 patients were included: group 1 (main) 28 patients with histologically verified high grade serous ovarian cancer of IIV FIGO stage, group 2 (control) 13 conditionally healthy women. Venous blood samples were collected immediately before the operation. Extracts of serum lipids were obtained in accordance with the modified Folch method. The composition of the samples was analyzed by electrospray ionization MS. Using the method of discriminant analysis and orthogonal projections to latent structures (OPLS-DA) were building OPLS-models based on profile of significant lipids. The comparison based on the non-parametric MannWhitney test. Results. The presence of 128 lipids in blood serum samples makes a major contribution to the OPLS-models, that are different for patients with IIV OC stage and controls. The OPLS-model parameters are: R2=0.87 and Q2=0.80, the area under the ROC curve reached 1, sensitivity and specificity of the model 100%. The second OPLS-model was developed to assign patients to 13 blood serum samples of the control group or to 5 blood samples of patients with I-II stages of OC: 108 lipids made the main contribution to this model (R2=0.97, Q2=0.86). The third OPLS-model was constructed to distinguish patients with earlier (IaIa stages; n=5) and advanced (IIaIVa; n=23) stages: R2=0.96 and Q2=1.00, AUC=0.99. Diglycerides, triglycerides, phosphatidylcholines, ethanolamines, sphingomyelins, ceramides, phosphatidylserines, phosphoinositols and prostaglandins significantly differ in the blood serum samples of patients with IaIc stages of OC and patients with IIIV stages and controls, that indicates the diagnostic value. Conclusion. It is possible to distinguish a healthy person from patient with IaIc or IIIV stages of OC. Serum oncolipids profile obtained by high-performance liquid chromatography with MS detection can be used as markers of early stages of OC, that are associated with better prognosis.
    Type of Medium: Online Resource
    ISSN: 2079-5831 , 2079-5696
    URL: Article
    DOI: 10.26442/20795696.2021.4.200911
    Language: Unknown
    Publisher: Consilium Medicum
    Publication Date: 2021
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Effect of activated glycyrrhizic acid on epithelial cell lipidome in HPV-associated cervical lesions: A prospective cohort study
    Consilium Medicum ; 2023
    In:  Gynecology Vol. 25, No. 2 ( 2023-07-14), p. 164-169
    Details
    In: Gynecology, Consilium Medicum, Vol. 25, No. 2 ( 2023-07-14), p. 164-169
    Abstract: Aim. To determine the change of lipid levels in the cervical epithelial cells during the treatment of chronic cervicitis and low-grade squamous intraepithelial lesions (LSIL) with 0.1% activated glycyrrhizic acid. Materials and methods. The prospective cohort study included 40 patients with chronic cervicitis and 40 patients with LSIL. Cytological evaluation of cervical smears was performed according to the Bethesda system (2014). All patients received Epigen Intim Spray for 18 months. Amplification of type-specific DNA fragments of human papillomavirus (HPV) and human DNA (sampling control SC) was done using a kit of reagents to detect, type, and quantitatively determine 21 HPV types by Quantum-21 HPV PCR. Lipid extract of a cervical epithelial scraping taken before and after therapy was analyzed by high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). Lipids were identified using the Lipid Match R-script and by characteristic MS/MS. Correlation analysis of the lipidome of cervicovaginal fluid and SC was performed using the Spearman test, and the Mann-Whitney test was used to compare lipid levels at the two time points. Results. During the treatment of chronic cervicitis, the levels of CL lipids 16:0_16:1_18:1_18:1, HexCer-NDS d20:0/26:0, PC 16: 0_18: 1 and 16: 0_20:4 were decreasing; during the treatment of LSIL, the levels of ceramides and glucosylceramides were decreasing. Chronic cervicitis is characterized by a positive correlation of SC smear with cardiolipins, glucosylceramides, and epithelial lipids with an ether bond; LSIL showed a negative correlation with phosphatidylcholines and phosphatidylethanolamines with an ether bond. Conclusion. As a result of treatment with activated glycyrrhizic acid, the lipid spectrum of epithelial cells significantly changed, depending on the type of lesion. It is also necessary to consider SC scrapings in further studies of epithelial cell lipidome and the type of HPV-associated lesions that affects the adjustments.
    Type of Medium: Online Resource
    ISSN: 2079-5831 , 2079-5696
    URL: Article
    DOI: 10.26442/20795696.2023.2.202252
    Language: Unknown
    Publisher: Consilium Medicum
    Publication Date: 2023
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...