GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Cold Spring Harbor Laboratory  (5)
  • 1
    Online Resource
    Online Resource
    Calcium Imaging of Neuronal Circuits In Vivo Using a Circuit-Tracing Pseudorabies Virus: Figure 1.
    Cold Spring Harbor Laboratory ; 2010
    In:  Cold Spring Harbor Protocols Vol. 2010, No. 4 ( 2010-04), p. pdb.prot5410-
    Details
    In: Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory, Vol. 2010, No. 4 ( 2010-04), p. pdb.prot5410-
    Abstract: Pseudorabies virus (PRV) is a neuroinvasive virus of the herpes family that has a broad host range but does not infect higher-order primates. PRV characteristically travels along chains of synaptically connected neurons and has been used extensively for elucidating neural circuits in the peripheral and central nervous system in vivo. The recombinant virus PRV369 is an attenuated retrograde tracer that encodes G-CaMP2, a fluorescent calcium sensor protein that is stable at physiological pH and mammalian temperature. This protocol describes the use of PRV369 to express G-CaMP2 in a neuronal circuit and to monitor its activity in a living animal, specifically in the submandibular ganglia (SMG), the peripheral parasympathetic ganglia that innervate the salivary glands. The procedure describes the delivery of PRV369 to the glands and shows how SMG neurons can then be imaged post-inoculation to explore connectivity and activity.
    Type of Medium: Online Resource
    ISSN: 1940-3402 , 1559-6095 , 1559-6095
    URL: Article
    DOI: 10.1101/pdb.prot5410
    Language: English
    Publisher: Cold Spring Harbor Laboratory
    Publication Date: 2010
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Cerebellar Craniotomy for In Vivo Calcium Imaging of Astrocytes
    Cold Spring Harbor Laboratory ; 2011
    In:  Cold Spring Harbor Protocols Vol. 2011, No. 10 ( 2011-10), p. pdb.prot065805-
    Details
    In: Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory, Vol. 2011, No. 10 ( 2011-10), p. pdb.prot065805-
    Abstract: The cerebellar cortex contains two astrocyte types: the Bergmann glia of the molecular layer and the velate protoplasmic astrocytes of the granule cell layer. In vivo, these cell types generate both subcellular calcium transients and trans-glial calcium waves. It is possible to perform in vivo calcium imaging in cerebellar astrocytes. One method involves injection of a replication-incompetent recombinant adenovirus for gene transfer of a fluorescent calcium indicator protein. A second method uses multicell bolus loading (MCBL) in the molecular layer of the cerebellum with synthetic calcium indicators. This protocol presents a cerebellar craniotomy procedure which can be used to prepare a virus-injected animal for in vivo imaging. It can also be used to prepare an animal for MCBL.
    Type of Medium: Online Resource
    ISSN: 1940-3402 , 1559-6095
    URL: Article
    DOI: 10.1101/pdb.prot065805
    Language: English
    Publisher: Cold Spring Harbor Laboratory
    Publication Date: 2011
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Preferential Loading of Bergmann Glia with Synthetic Acetoxymethyl Calcium Dyes: Figure 1.
    Cold Spring Harbor Laboratory ; 2011
    In:  Cold Spring Harbor Protocols Vol. 2011, No. 10 ( 2011-10), p. pdb.prot065813-
    Details
    In: Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory, Vol. 2011, No. 10 ( 2011-10), p. pdb.prot065813-
    Abstract: The cerebellar cortex contains two astrocyte types: the Bergmann glia of the molecular layer and the velate protoplasmic astrocytes of the granule cell layer. In vivo, these cell types generate both subcellular calcium transients and trans-glial calcium waves. This protocol outlines a method for in vivo calcium imaging in cerebellar astrocytes of mice which have undergone a cerebellar craniotomy. Multicell bolus loading (MCBL) is performed using the synthetic calcium indicators Fluo-5F AM and Fluo-4 AM. In the cerebellum, a degree of cell-type specificity can be achieved by varying the depth of injection. This protocol describes a loading procedure following craniotomy which allows preferential labeling of Bergmann glia.
    Type of Medium: Online Resource
    ISSN: 1940-3402 , 1559-6095
    URL: Article
    DOI: 10.1101/pdb.prot065813
    Language: English
    Publisher: Cold Spring Harbor Laboratory
    Publication Date: 2011
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Translational regulation of glutathione peroxidase 4 expression through guanine-rich sequence-binding factor 1 is essential for embryonic brain development
    Cold Spring Harbor Laboratory ; 2008
    In:  Genes & Development Vol. 22, No. 13 ( 2008-07-01), p. 1838-1850
    Details
    In: Genes & Development, Cold Spring Harbor Laboratory, Vol. 22, No. 13 ( 2008-07-01), p. 1838-1850
    Abstract: Phospholipid hydroperoxide glutathione peroxidase (GPx4) is a moonlighting selenoprotein, which has been implicated in basic cell functions such as anti-oxidative defense, apoptosis, and gene expression regulation. GPx4-null mice die in utero at midgestation, and developmental retardation of the brain appears to play a major role. We investigated post-transcriptional mechanisms of GPx4 expression regulation and found that the guanine-rich sequence-binding factor 1 (Grsf1) up-regulates GPx4 expression. Grsf1 binds to a defined target sequence in the 5′-untranslated region (UTR) of the mitochondrial GPx4 (m-GPx4) mRNA, up-regulates UTR-dependent reporter gene expression, recruits m-GPx4 mRNA to translationally active polysome fractions, and coimmunoprecipitates with GPx4 mRNA. During embryonic brain development, Grsf1 and m-GPx4 are coexpressed, and functional knockdown (siRNA) of Grsf1 prevents embryonic GPx4 expression. When compared with mock controls, Grsf1 knockdown embryos showed significant signs of developmental retardations that are paralleled by apoptotic alterations (TUNEL staining) and massive lipid peroxidation (isoprostane formation). Overexpression of m-GPx4 prevented the apoptotic alterations in Grsf1-deficient embryos and rescued them from developmental retardation. These data indicate that Grsf1 up-regulates translation of GPx4 mRNA and implicate the two proteins in embryonic brain development.
    Type of Medium: Online Resource
    ISSN: 0890-9369 , 1549-5477
    URL: Article
    DOI: 10.1101/gad.466308
    RVK:
    WA 15000
    Language: English
    Publisher: Cold Spring Harbor Laboratory
    Publication Date: 2008
    detail.hit.zdb_id: 1467414-2
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Injection of Recombinant Adenovirus for Delivery of Genetically Encoded Calcium Indicators into Astrocytes of the Cerebellar Cortex: Figure 1.
    Cold Spring Harbor Laboratory ; 2011
    In:  Cold Spring Harbor Protocols Vol. 2011, No. 10 ( 2011-10), p. pdb.prot065797-
    Details
    In: Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory, Vol. 2011, No. 10 ( 2011-10), p. pdb.prot065797-
    Abstract: The cerebellar cortex contains two astrocyte types: the Bergmann glia of the molecular layer and the velate protoplasmic astrocytes of the granule cell layer. In vivo, these cell types generate both subcellular calcium transients and trans-glial calcium waves. This protocol outlines a method for in vivo calcium imaging in cerebellar astrocytes, using the injection of a replication-incompetent recombinant adenovirus for gene transfer of the fluorescent calcium indicator protein (FCIP) G-CaMP2. The adenovirus contains a cytomegalovirus (CMV) immediate-early (IE) promoter which confines expression of G-CaMP2 to astrocytes. Expression is sufficiently high to allow calcium signals to be recorded in Bergmann glial processes as well as the processes and somata of velate protoplasmic astrocytes. To obtain structural information, G-CaMP2 fused with the brighter chromophore DsRed allows three-dimensional (3D) reconstruction of cells. G-CaMP2 expression lasts for at least 3 wk, enabling long-term functional imaging in both anesthetized and awake animals.
    Type of Medium: Online Resource
    ISSN: 1940-3402 , 1559-6095
    URL: Article
    DOI: 10.1101/pdb.prot065797
    Language: English
    Publisher: Cold Spring Harbor Laboratory
    Publication Date: 2011
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...