In:
Pathogens and Immunity, Case Western Reserve University, Vol. 2, No. 1 ( 2017-02-01), p. 23-
Abstract:
Molecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST). In addition, we suggest that MLST may have higher discriminative power compared to hsp60 sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.
Type of Medium:
Online Resource
ISSN:
2469-2964
DOI:
10.20411/pai.v1i2.99
Language:
Unknown
Publisher:
Case Western Reserve University
Publication Date:
2017
detail.hit.zdb_id:
3032410-5
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