In:
Biochemistry and Cell Biology, Canadian Science Publishing, Vol. 76, No. 5 ( 1998-10-01), p. 787-790
Abstract:
A mutant of Proteus mirabilis had been previously isolated as defective in swarming. The mutation had been found to be in a gene related to the Escherichia coli zntA gene, which encodes the ZntA Zn(II)-translocating P-type ATPase. In this study the P. mirabilis genewas expressed in an E. coli strain in which the zntA gene had been disrupted. The P. mirabilis gene complemented the sensitivity to salts of zinc and cadmium. Everted membrane vesicles from the zntA-disrupted strain lost ATP-driven 65 Zn(II) uptake. Membranes from the complemented strain had restored 65 Zn(II) transport. These results demonstrate that the P. mirabilis homologue of ZntA is a Zn(II)-translocating P-type ATPase.Key words: zinc resistance, P-type ATPase, Proteus mirabilis.
Type of Medium:
Online Resource
ISSN:
0829-8211
,
1208-6002
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
1998
SSG:
12
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