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  • Canadian Science Publishing  (2)
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  • Canadian Science Publishing  (2)
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  • 1
    Online Resource
    Online Resource
    A Culture Technique Using Marine Fish Kidney to Obtain Chromosomes
    Canadian Science Publishing ; 1979
    In:  Journal of the Fisheries Research Board of Canada Vol. 36, No. 4 ( 1979-04-01), p. 458-461
    Details
    In: Journal of the Fisheries Research Board of Canada, Canadian Science Publishing, Vol. 36, No. 4 ( 1979-04-01), p. 458-461
    Abstract: A relatively simple and reliable in vitro method for marine fish chromosome study was developed. The addition of 10% chick embryo extract to serum-supplemented Eagle's minimum essential medium with high concentration of NaCl resulted in marked growth of kidney cells in the marine conger eel (Astroconger myriaster) after activation by phytohemagglutinin (PHA). Culture medium without chick embryo extract or PHA and/or with normal concentration of NaCl did not induce substantial growth. In contrast to reports by others, humidified culture was not required for excellent cell growth of these teleost kidney cells. Numerous metaphases unmarred by overlapping chromosomes were recovered and excellent karyograms were available for detailed karyotype analysis. Key words: kidney, culture, marine fish, chromosome
    Type of Medium: Online Resource
    ISSN: 0015-296X
    URL: Article
    DOI: 10.1139/f79-063
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1979
    detail.hit.zdb_id: 3036-3
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  • 2
    Online Resource
    Online Resource
    STUDIES ON THE EFFECTS OF STEROIDS ON DNA SYNTHESIS OF HUMAN CHROMOSOMES IN VITRO
    Canadian Science Publishing ; 1968
    In:  Canadian Journal of Genetics and Cytology Vol. 10, No. 2 ( 1968-06-01), p. 299-310
    Details
    In: Canadian Journal of Genetics and Cytology, Canadian Science Publishing, Vol. 10, No. 2 ( 1968-06-01), p. 299-310
    Abstract: Tritiated thymidine and autoradiographic techniques were used to investigate the sequence and pattern of DNA replication of sex chromosomes in cultured human cells treated with progesterone and testosterone.The average frequencies of progesterone- and testosterone-induced chromatid aberration were 19 and 26% respectively, whereas that of spontaneous aberration was 2.5%. No chromosome aberrations were found in sex chromosomes of either sex. Aberrations were distributed throughout cells in both S and G 2 stages. The general production of aberrations increased with increases in the time between isotopic labeling and fixation. The mean duration of G 2 was over 3 hours in the control, over 4.5 hours in the progesterone-treated group and 4.75 hours in the testosterone group. Thus, G 2 time for human kidney cells treated with steroids was delayed about 1 to 2 hours.Striking asynchrony of DNA duplication in homologous X chromosomes in steroids-treated female cells was also observed at the end of the period. However, even in metaphases that incorporated thymidine-H 3 at 6 hours before fixation the hot-X was detectable. The Y chromosome in male cells showed a high percentage of labeling intensity with particularly heavy grains, differing from that of G group. Metaphases of female cells labelled at 6 hours before fixation in progesterone had an unlabeled, chromosome in the complement of moderately labeled cells. This unlabeled chromosome was also found in testosterone-treated cells prepared 10 hours after labeling and is presumed to be heteropycnotic X chromosome.
    Type of Medium: Online Resource
    ISSN: 0008-4093
    URL: Article
    DOI: 10.1139/g68-042
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1968
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