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  • Canadian Science Publishing  (5)
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  • Canadian Science Publishing  (5)
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  • 1
    Online Resource
    Online Resource
    Ultrastructure of Pyramimonas cyrtoptera sp.nov. (Prasinophyceae), a species with 16 flagella from northern Foxe Basin, Arctic Canada, including observations on growth rates
    Canadian Science Publishing ; 1992
    In:  Canadian Journal of Botany Vol. 70, No. 6 ( 1992-06-01), p. 1259-1273
    Details
    In: Canadian Journal of Botany, Canadian Science Publishing, Vol. 70, No. 6 ( 1992-06-01), p. 1259-1273
    Abstract: A new species of the genus Pyramimonas is reported from the Canadian Arctic, the first record of Pyramimonas from northern Foxe Basin, Northwest Territories. The general structure of the cell and six types of scales are described. Pyramimonas cyrtoptera sp.nov. is remarkable in possessing 16 flagella, two chloroplasts, two pyrenoids, and two pairs of eyespots of unequal size. It is the largest known species of Pyramimonas, measuring 38–42 μm in length. The presence of 16 flagella and two chloroplasts sets it apart from all other species of Pyramimonas. Pyramimonas cyrtoptera belongs to the subgenus Pyramimonas. A new type of hair scale is reported for members of this group. The growth responses of P. cyrtoptera to variations in temperature and salinity indicate that it is as cold stenothermal and euryhaline. Key words: Prasinophyceae, Pyramimonas, flagella, Arctic Canada, temperature, salinity.
    Type of Medium: Online Resource
    ISSN: 0008-4026
    URL: Article
    DOI: 10.1139/b92-159
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1992
    detail.hit.zdb_id: 218116-2
    detail.hit.zdb_id: 1481926-0
    SSG: 12
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Ultrastructural studies on Cymbomonas tetramitiformis (Prasinophyceae). I. General structure, scale microstructure, and ontogeny
    Canadian Science Publishing ; 2003
    In:  Canadian Journal of Botany Vol. 81, No. 7 ( 2003-07-01), p. 657-671
    Details
    In: Canadian Journal of Botany, Canadian Science Publishing, Vol. 81, No. 7 ( 2003-07-01), p. 657-671
    Abstract: The marine prasinophycean flagellate Cymbomonas tetramitiformis has been established in culture from Japan and Australia and is examined in detail by electron microscopy. The results are described as a series of reports, the first of which deals with the general cell structure and the structure and morphogenesis of the organic scales that cover the surface of the cell and the flagella. The cell produces seven types of organic scales, all of which are manufactured in the cisternae of the Golgi apparatus. Two scale types are deposited on the cell body and cover the cell in three close-fitting layers, two types are confined to the flagellar pit region, and three types cover the flagellar surfaces. Cymbomonas is quadriflagellate and closely related to another quadriflagellate, Pyramimonas, in scale structure and scale ontogeny. It differs markedly in cell symmetry, swimming behavior, and in details of the flagellar apparatus. The flagellar end of the cell with the flagellar pit is surrounded by a horseshoe-shaped rim, which is very different from the usually four-lobed anterior end of Pyramimonas. Cymbomonas also shows resemblance in scale structure to members of the order Mamiellales, notably Mamiella, and to the phycoma-producing genera Pterosperma and Halosphaera. The Japanese isolate of Cymbomonas formed cysts for a short period, and the cyst appears to be homologous with the so-called phycoma stage of Pterosperma and Halosphaera. Since two chloroplasts were seen in the cysts, the cysts probably arise as a result of sexual reproduction. Concomitant with the presence of cysts in the culture, tiny uniflagellate cells were also present, and they probably represent gametes. Cymbomonas was described from the Adriatic in 1913 and was not reported again anywhere until 1986 and 1987 when Throndsen refound it in the Gulf of Naples in southern Italy. It has subsequently been found in large parts of the world and is now readily recognized by its characteristic scales and mode of swimming. It is often common but does not appear to form blooms.Key words: Prasinophyceae, marine nanoplankton, ultrastructure, Cymbomonas.
    Type of Medium: Online Resource
    ISSN: 0008-4026
    URL: Article
    DOI: 10.1139/b03-055
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2003
    detail.hit.zdb_id: 218116-2
    detail.hit.zdb_id: 1481926-0
    SSG: 12
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    Online Resource
  • 3
    Online Resource
    Online Resource
    Toxicity tests in eight species of Chrysochromulina (Haptophyta)
    Canadian Science Publishing ; 1997
    In:  Canadian Journal of Botany Vol. 75, No. 1 ( 1997-01-01), p. 129-136
    Details
    In: Canadian Journal of Botany, Canadian Science Publishing, Vol. 75, No. 1 ( 1997-01-01), p. 129-136
    Abstract: Blooms of the marine flagellate Chrysochromulina have resulted in mortality of marine organisms in Scandinavian waters, including fish in aquaculture. Eight species of Chrysochromulina, namely C. apheles, C. brevifilum, C. ericina, C. hirta, C. leadbeateri, C. parva, C. polylepis, and C. simplex, isolated into unialgal culture, were examined for haemolytic activity and toxicity to the brine shrimp, Artemia salina. Haemolytic fractions were obtained from all species, but only C. polylepis cells were toxic to Artemia. Thin-layer chromatography (TLC) analysis in chloroform –methanol–water (75:25:4) and in chloroform–methanol (9:1) yielded up to six haemolytic spots. Except for one spot, these all occurred in extracts of the species examined, including Isochrysis sp., which was used as a control, C. polylepis, and the well-known fish killer Prymnesium parvum. The single unique haemolytic spot (R f values 0.45 and 0.16 in solvents I and II, respectively) occurred in the extract from C. polylepis. When isolated by TLC, the contents of the single spot were toxic to Artemia. Key words: Chrysochromulina, toxicity, haemolytic, Artemia, thin-layer chromatography (TLC).
    Type of Medium: Online Resource
    ISSN: 0008-4026
    URL: Article
    DOI: 10.1139/b97-015
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1997
    detail.hit.zdb_id: 218116-2
    detail.hit.zdb_id: 1481926-0
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Ultrastructure and reconstruction of the flagellar apparatus in Chrysochromulina apheles sp. nov. (Prymnesiophyceae = Haptophyceae)
    Canadian Science Publishing ; 1986
    In:  Canadian Journal of Botany Vol. 64, No. 3 ( 1986-03-01), p. 593-610
    Details
    In: Canadian Journal of Botany, Canadian Science Publishing, Vol. 64, No. 3 ( 1986-03-01), p. 593-610
    Abstract: A new marine species of Chrysochromulina, C. apheles, is described from light and electron microscopy of a culture established from Danish coastal waters. The cells are among the smallest known in any species of Chrysochromulina, measuring ca. 4 μm in diameter. The general fine structure is illustrated and the structure of the haptonema and the flagellar apparatus is described in detail, based on serial sections. The flagellar root system, not previously examined in detail in any member of Chrysochromulina, is shown to consist of four microtubular roots, while cross-banded roots are lacking. Four cross-banded fibres were seen to interconnect the flagellar bases and the haptonema base. The haptonema belongs to the rather unusual six-stranded type. Two very similar looking types of small organic scales are present on the cell body. Unpublished data on the flagellar roots of the type species of Chrysochromulina, C. parva, are included. Chrysochromulina apheles is apparently cosmopolitan. It has presently been found in material from Denmark, Finland, England, Thailand, Australia, and New Zealand.
    Type of Medium: Online Resource
    ISSN: 0008-4026
    URL: Article
    DOI: 10.1139/b86-077
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1986
    detail.hit.zdb_id: 218116-2
    detail.hit.zdb_id: 1481926-0
    SSG: 12
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    Online Resource
  • 5
    Online Resource
    Online Resource
    Light and electron microscopical studies on Pseudoscourfieldia marina , a primitive scaly green flagellate (Prasinophyceae) with posterior flagella
    Canadian Science Publishing ; 1988
    In:  Canadian Journal of Botany Vol. 66, No. 7 ( 1988-07-01), p. 1415-1434
    Details
    In: Canadian Journal of Botany, Canadian Science Publishing, Vol. 66, No. 7 ( 1988-07-01), p. 1415-1434
    Abstract: The supposedly primitive prasinophyte Pseudoscourfieldia marina has been established in culture and examined in detail. The cells swim by means of two unequal but parallel posterior flagella. Pseudoscourfieldia differs from other green flagellates in the structure of the flagellar root system, possession of a conspicuous leaflike attenuated extension from one basal body into the cell, the structure of the pyrenoid, which is invaginated by long extensions from the mitochondrial complex, and the structure of the scaly covering on the body. Pseudoscourfieldia shows distinct similarities to Nephroselmis, which is laterally biflagellate, and to the Tetraselmis group, which possesses four anterior flagella. The attachment of the hair scales on the flagella has been examined in detail for the first time in a green flagellate. The very short hair scales attach to the flagellar surface between each pair of longitudinal rows of scales known as Melkonian's rows, associated with the inside of every third scale in one row. This is probably a feature common to all prasinophytes with Melkonian's rows. Based on the many investigations now published, a classification for the prasinophytes, supposedly some of the most primitive green algae, is suggested. One class, two orders, and four families are recognized and defined, with 15 genera.
    Type of Medium: Online Resource
    ISSN: 0008-4026
    URL: Article
    DOI: 10.1139/b88-197
    Language: French
    Publisher: Canadian Science Publishing
    Publication Date: 1988
    detail.hit.zdb_id: 218116-2
    detail.hit.zdb_id: 1481926-0
    SSG: 12
    Location Call Number Limitation Availability
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