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  • Canadian Science Publishing  (4)
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  • Canadian Science Publishing  (4)
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  • 1
    In: Genome, Canadian Science Publishing, Vol. 51, No. 4 ( 2008-04), p. 303-308
    Abstract: xs1 is a male-sterile rice mutant derived from a spontaneous mutation. The floret of the mutant, consisting of 6 stamens and 1 pistil, looks the same as that of the wild type except that the filaments are long and thin and the anthers are withered in white transparence. It is confirmed that xs1 is a no-pollen type of male-sterile mutant, for no pollen grains can be stained with I 2 –KI solution and the anther locules are always hollow. Anther transverse sections indicate that the mutant microspores are abnormally condensed and agglomerated to form a deeply stained cluster at the late microspore stage, which results in cessation of the vacuolation process of microspores, and, therefore, the mutant forms no functional pollens for reproduction. Genetic analysis of 4 F 2 populations and 3 BC 1 F 1 populations revealed that the mutation is controlled by a single recessive gene, termed VR1 (Vacuolation retardation 1). Screening of 432 F 2 mutant individuals derived from the cross of xs1 × G603 with simple sequence repeat markers revealed that VR1 is located between the molecular markers RM17411 and RM5030, at distances of 0.7 and 1.5 cM, respectively, on chromosome 4. VR1 is a new male fertility controlling gene located on chromosome 4 in rice.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2008
    detail.hit.zdb_id: 2020635-5
    SSG: 12
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  • 2
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 56, No. 2 ( 2010-02), p. 156-161
    Abstract: We characterized a novel holotype cry gene (cry52Ba1) harbored in a Bacillus thuringiensis isolate BM59-2 native to China that expresses a spherical crystal protein of about 80 kDa. In this study, the full length of the cry52Ba1 gene was cloned from this strain. Sequence analysis of the gene was also performed. Furthermore, cry52Ba1 was expressed in Escherichia coli BL21(DE)pLysS, and the resulting insecticidal activity showed that the Cry52Ba1 protein exhibited high larvicidal activity against Aedes aegypti (Diptera), with a lethal concentration 50 of 1.526 µg/mL (95% confidence: 0.740–3.499 µg/mL).
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2010
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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  • 3
    In: Canadian Journal of Plant Science, Canadian Science Publishing, Vol. 100, No. 4 ( 2020-08-01), p. 425-434
    Abstract: Grain protein content (GPC) is an important nutritional quality trait of wheat. Aegilops tauschii Coss. is a progenitor of common wheat and has been shown to have high GPC. The objective of this study was to identify quantitative trait loci (QTL) for GPC using A. tauschii–Triticum aestivum L. recombinant lines. An advanced BC 2 F 6 population (112 lines) containing A. tauschii segments was developed using synthetic octaploid wheat (hexaploid wheat Zhoumai 18 × A. tauschii T093), which displayed significant phenotype variances. Two quality traits, GPC and wet gluten, and four yield-related traits, thousand kernel weight, spikelet number per plant, grain number per spike, and grain weight per spike, were evaluated. The results show that the mean GPCs of these lines were significantly higher than those of Zhoumai 18. Correlation and mapping analyses indicated that quality traits were weakly negatively correlated with yield traits. Furthermore, 16 A. tauschii-derived QTL for GPC were detected in the recombinant inbred lines, and four stable QTL that have no significant negative effects on yield and are located within the same marker interval were detected in both environments. Additionally, high-protein, high-yield lines 150228 and 150368 with stable QTL were obtained, and both can be directly utilised for fine mapping of the GPC genes and molecular marker–assisted selection to achieve synergistic improvement of wheat yield and protein content.
    Type of Medium: Online Resource
    ISSN: 0008-4220 , 1918-1833
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2020
    detail.hit.zdb_id: 2016989-9
    SSG: 12
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  • 4
    In: Genome, Canadian Science Publishing, Vol. 50, No. 1 ( 2007-01), p. 72-83
    Abstract: Yunnan Province is one of the largest centers of genetic diversity of Oryza sativa L. in China, and in the world. Using a genetically representative core collection of 692 rice landraces from Yunnan, the genetic structure, differentiation, and geographic diversity of this rice germplasm were analyzed. The accessions were classified into different populations, according to the model-based structure analysis. Model-based populations were characterized through the reconstruction of a neighbor-joining tree, principal coordinate analysis, and the estimation of morphologic and SSR variations. Finally, the distribution of genetic diversity and differentiation among districts were studied. Seven model-based populations were identified on the basis of the structure analysis. This classification was partly consistent with Ting’s 5-level taxonomic system. Differentiation between 2 rice subspecies is the most apparent, with a clearer differentiation between soil-watery ecotypes in japonica than in indica; however, differentiation among seasonal ecotypes in indica is clearer than in japonica. Cropping system and man-made restricted-growth environments could be considered to be the main forces driving the intraspecific differentiation of cultivated rice. It has been suggested that, because it possesses the highest genetic diversity and all the necessary conditions as a center of genetic diversity, the southwest region of Yunnan, encompassing Simao, Lincang, and Xishuangbanna districts, is the center of genetic diversity of Yunnan rice landraces.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2007
    detail.hit.zdb_id: 2020635-5
    SSG: 12
    Location Call Number Limitation Availability
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