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  • 1
    Online Resource
    Online Resource
    Genomic in situ hybridization (GISH) reveals high chromosome pairing affinity between 〈i〉Lolium perenne〈/i〉 and 〈i〉Festuca mairei〈/i〉
    Canadian Science Publishing ; 2000
    In:  Genome Vol. 43, No. 2 ( 2000), p. 398-403
    Details
    In: Genome, Canadian Science Publishing, Vol. 43, No. 2 ( 2000), p. 398-403
    Type of Medium: Online Resource
    ISSN: 1480-3321 , 0831-2796
    URL: Article
    DOI: 10.1139/gen-43-2-398
    Language: Unknown
    Publisher: Canadian Science Publishing
    Publication Date: 2000
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  • 2
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    Online Resource
    Application of fiber-FISH in physical mapping of 〈i〉Arabidopsis thaliana〈/i〉
    Canadian Science Publishing ; 1998
    In:  Genome Vol. 41, No. 4 ( 1998), p. 566-572
    Details
    In: Genome, Canadian Science Publishing, Vol. 41, No. 4 ( 1998), p. 566-572
    Type of Medium: Online Resource
    ISSN: 1480-3321 , 0831-2796
    URL: Article
    DOI: 10.1139/gen-41-4-566
    Language: Unknown
    Publisher: Canadian Science Publishing
    Publication Date: 1998
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  • 3
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    Online Resource
    QTL detection for rice grain quality traits using an interspecific backcross population derived from cultivated Asian ( O. sativa L.) and African ( O. glaberrima S.) rice
    Canadian Science Publishing ; 2004
    In:  Genome Vol. 47, No. 4 ( 2004-08-01), p. 697-704
    Details
    In: Genome, Canadian Science Publishing, Vol. 47, No. 4 ( 2004-08-01), p. 697-704
    Abstract: An interspecific advanced backcross population derived from a cross between Oryza sativa 'V20A' (a popular male-sterile line used in Chinese rice hybrids) and Oryza glaberrima (accession IRGC No. 103544 from Mali) was used to identify quantitative trait loci (QTL) associated with grain quality and grain morphology. A total of 308 BC 3 F 1 hybrid families were evaluated for 16 grain-related traits under field conditions in Changsha, China, and the same families were evaluated for RFLP and SSR marker segregation at Cornell University (Ithaca, N.Y.). Eleven QTL associated with seven traits were detected in six chromosomal regions, with the favorable allele coming from O. glaberrima at eight loci. Favorable O. glaberrima alleles were associated with improvements in grain shape and appearance, resulting in an increase in kernel length, transgressive variation for thinner grains, and increased length to width ratio. Oryza glaberrima alleles at other loci were associated with potential improvements in crude protein content and brown rice yield. These results suggested that genes from O. glaberrima may be useful in improving specific grain quality characteristics in high-yielding O. sativa hybrid cultivars.Key words: quantitative trait loci (QTL), grain quality, molecular markers, O. sativa, O. glaberrima.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    URL: Article
    DOI: 10.1139/g04-029
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2004
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  • 4
    Online Resource
    Online Resource
    Construction of a bacterial artificial chromosome (BAC) library for potato molecular cytogenetics research
    Canadian Science Publishing ; 2000
    In:  Genome Vol. 43, No. 1 ( 2000-02-01), p. 199-204
    Details
    In: Genome, Canadian Science Publishing, Vol. 43, No. 1 ( 2000-02-01), p. 199-204
    Abstract: Lack of reliable techniques for chromosome identification is the major obstacle for cytogenetics research in plant species with large numbers of small chromosomes. To promote molecular cytogenetics research of potato (Solanum tuberosum, 2n = 4x = 48) we developed a bacterial artificial chromosome (BAC) library of a diploid potato species S. bulbocastanum. The library consists of 23 808 clones with an average insert size of 155 kb, and represents approximately 3.7 equivalents to the potato genome. The majority of the clones in the BAC library generated distinct signals on specific potato chromosomes using fluorescence in situ hybridization (FISH). The hybridization signals provide excellent cytological markers to tag individual potato chromosomes. We also demonstrated that the BAC clones can be mapped to specific positions on meiotic pachytene chromosomes. The excellent resolution of pachytene FISH can be used to construct a physical map of potato by mapping molecular marker-targeted BAC clones on pachytene chromosomes. Key words: potato, BAC library, chromosome identification, physical mapping, molecular cytogenetics.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    URL: Article
    DOI: 10.1139/g99-099
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2000
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  • 5
    Online Resource
    Online Resource
    Detection of intergenomic translocations with centromeric and noncentromeric breakpoints in Triticum araraticum : mechanism of origin and adaptive significance
    Canadian Science Publishing ; 1995
    In:  Genome Vol. 38, No. 5 ( 1995-10-01), p. 976-981
    Details
    In: Genome, Canadian Science Publishing, Vol. 38, No. 5 ( 1995-10-01), p. 976-981
    Abstract: Triticum araraticum Jakubz. (2n = 4x = 28, A t A t GG), a wild progenitor of the polyploid cultivated wheat T. timopheevii, shows extensive chromosome translocation polymorphism in natural populations from the Middle East and Transcaucasia. From an extensive survey, eight intergenomic translocation types were observed and their breakpoints analyzed by genomic in situ hybridization. The previously reported species-specific 6A t –1G–4G cyclic translocation was found in all accessions studied. In four translocation types, the breakpoints were in interstitial regions of chromosomes and the other four arose via centric–breakage–fusion. A model is presented on the mechanism of origin and the adaptive significance of translocations with centromeric and noncentromeric breakpoints.Key words: intraspecific diversity, intergenomic translocations, Triticum araraticum.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    URL: Article
    DOI: 10.1139/g95-128
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1995
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  • 6
    Online Resource
    Online Resource
    Chronic effects of venlafaxine on synaptophysin and neuronal cell adhesion molecule in the hippocampus of cerebral ischemic miceThis paper is one of a selection of papers published in this special issue entitled “Second International Symposium on Recent Advances in Basic, Clinical, and Social Medicine” and has undergone the Journal's usual peer review process.
    Canadian Science Publishing ; 2010
    In:  Biochemistry and Cell Biology Vol. 88, No. 4 ( 2010-08), p. 655-663
    Details
    In: Biochemistry and Cell Biology, Canadian Science Publishing, Vol. 88, No. 4 ( 2010-08), p. 655-663
    Abstract: Venlafaxine, a novel antidepressant, inhibits serontonin and norepinephrine reuptake in the presynaptic cleft. Unlike typical selective serontonin reuptake inhibitors (SSRIs), venlafaxine may have modulatory effects on nerve terminals and neuronal plasticity. Our preliminary data found that 5 mg·kg –1 ·d –1 of venlafaxine treatment prevented decreased synaptophysin (SYP) in the hippocampus, which results from chronic restrained stress in the rat model. The present study investigates whether venlafaxine regulates alterations of synaptophysin and neuronal cell adhesion molecule (NCAM) in a post-stroke depression mouse model. We compared the expression level of SYP and NCAM in the hippocampus of global cerebral ischemic (GCI) mice treated with different doses of venlafaxine using immunohistological and Western blot analysis. Pre-treatment with intraperitoneal injection of venlafaxine (2.5 and 5.0 mg·kg –1 ·d –1 ) for 14 days significantly prevented the decrease of SYP in the hilus area of the hippocampus in vehicle-treated GCI mice. NCAM was significantly higher in the hippocampus of vehicle-treated GCI mice, and pretreatment with venlafaxine prevented alterations of NCAM, with the high-dose venlafaxine group comparable with vehicle-sham mice. The results suggest the alteration of neuronal remodeling proteins in the hippocampus may be an underlying mechanism of venlafaxine in treating post-stroke depression.
    Type of Medium: Online Resource
    ISSN: 0829-8211 , 1208-6002
    URL: Article
    DOI: 10.1139/O10-015
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2010
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  • 7
    Online Resource
    Online Resource
    Alloplasmic wheat – Elymus ciliaris chromosome addition lines
    Canadian Science Publishing ; 1993
    In:  Genome Vol. 36, No. 2 ( 1993-04-01), p. 327-333
    Details
    In: Genome, Canadian Science Publishing, Vol. 36, No. 2 ( 1993-04-01), p. 327-333
    Abstract: Alloplasmic euploid wheat with the cytoplasm of Elymus ciliaris (2n = 4x = 28, S c S c Y c Y c ) is male sterile and has reduced vigor. However, alloplasmic plants with E. ciliaris chromosomes 1S c or 1Y c marked by gliadin genes Gli-S c 1 and Gli-Y c l, respectively, are vigorous and fertile. The Rf genes on 1S c and 1Y c are named Rf-S c 1 and Rf-Y c 1. Two chromosome translocations involving 1Y c were isolated. The first involved the short arm of 1Y c translocated to the short arm of wheat chromosome 3B. The second involved the short arm of 1Y c translocated to the short arm of a chromosome, designated L, of E. ciliaris. The second line also has another E. ciliaris chromosome designated A and lacks wheat chromosome 6A. This line is resistant to Puccinia recondita. The relationship between fertility restoration and nucleolar organizing regions is discussed. Key words : Triticum aestivum, Elymus ciliaris, chromosome addition, Rf genes, nucleolar organizing regions.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    URL: Article
    DOI: 10.1139/g93-045
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1993
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  • 8
    Online Resource
    Online Resource
    Genomic in situ hybridization (GISH) reveals high chromosome pairing affinity between Lolium perenne and Festuca mairei
    Canadian Science Publishing ; 2000
    In:  Genome Vol. 43, No. 2 ( 2000-03-15), p. 398-403
    Details
    In: Genome, Canadian Science Publishing, Vol. 43, No. 2 ( 2000-03-15), p. 398-403
    Abstract: Intergeneric hybridizations have been made between species of Lolium and Festuca. It has been demonstrated, largely through conventional cytogenetic analysis, that the genomes of the two genera are related, however, much information is lacking on exactly how closely related the genomes are between the two species. We applied genomic in situ hybridization (GISH) techniques to the F 1 hybrids of tetraploid Festuca mairei with a genomic constitution of M 1 M 1 M 2 M 2 and diploid Lolium perenne with a genomic constitution of LL. It was shown in the triploid hybrids (LM 1 M 2 ) that the chromosomes of M 1 and M 2 from F. mairei could pair with each other, and it was further discovered that L chromosomes of L. perenne paired with M 1 and M 2 chromosomes. Our results showed that meiocytes of Lolium-Festuca are amenable to GISH analysis, and provided direct evidence for the hypothesis that the chromosomes of Lolium and Festuca may be genetically equivalent and that reciprocal mixing of the genomes may be possible. Key words: Lolium, Festuca, in situ hybridization, meiosis.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    URL: Article
    DOI: 10.1139/g99-129
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2000
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  • 9
    Online Resource
    Online Resource
    Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research
    Canadian Science Publishing ; 2006
    In:  Genome Vol. 49, No. 9 ( 2006-09), p. 1057-1068
    Details
    In: Genome, Canadian Science Publishing, Vol. 49, No. 9 ( 2006-09), p. 1057-1068
    Abstract: Fluorescence in situ hybridization (FISH), which allows direct mapping of DNA sequences on chromosomes, has become the most important technique in plant molecular cytogenetics research. Repetitive DNA sequence can generate unique FISH patterns on individual chromosomes for karyotyping and phylogenetic analysis. FISH on meiotic pachytene chromosomes coupled with digital imaging systems has become an efficient method to develop physical maps in plant species. FISH on extended DNA fibers provides a high-resolution mapping approach to analyze large DNA molecules and to characterize large genomic loci. FISH-based physical mapping provides a valuable complementary approach in genome sequencing and map-based cloning research. We expect that FISH will continue to play an important role in relating DNA sequence information to chromosome biology. FISH coupled with immunoassays will be increasingly used to study features of chromatin at the cytological level that control expression and regulation of genes.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    URL: Article
    DOI: 10.1139/g06-076
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2006
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  • 10
    Online Resource
    Online Resource
    A bacterial artificial chromosome (BAC) library of Malus floribunda 821 and contig construction for positional cloning of the apple scab resistance gene Vf
    Canadian Science Publishing ; 2001
    In:  Genome Vol. 44, No. 6 ( 2001-12-01), p. 1104-1113
    Details
    In: Genome, Canadian Science Publishing, Vol. 44, No. 6 ( 2001-12-01), p. 1104-1113
    Abstract: The apple scab resistance gene Vf, originating from the wild species Malus floribunda 821, has been incorporated into a wide variety of apple cultivars through a classical breeding program. With the aim of isolating the Vf gene, a bacterial artificial chromosome (BAC) library consisting of 31 584 clones has been constructed from M. floribunda 821. From the analysis of 88 randomly selected BAC clones, the average insert size is estimated at 125 kb. If it is assumed that the genome size of M. floribunda 821 is 769 Mb/haploid, the library represents about 5× haploid genome equivalents. This provides a 99% probability of finding any specific sequence from this library. PCR-based screening of the library has been carried out using eight random genomic sequence-characterized amplified regions (SCARs), chloroplast- and mitochondria-specific SCARs, and 13 high-density Vf-linked SCAR markers. An average of five positive BAC clones per random SCAR has been obtained, whereas less than 1% of BAC clones are derived from the chloroplast or mitochondrial genomes. Most BAC clones identified with Vf-linked SCAR markers are physically linked. Three BAC contigs along the Vf region have been obtained by assembling physically linked BAC clones based on their fingerprints. The overlapping relatedness of BAC clones has been further confirmed by cytogenetic mapping using fiber fluorescence in situ hybridization (fiber-FISH). The M. floribunda 821 BAC library provides a valuable genetic resource not only for map-based cloning of the Vf gene, but also for finding many other important genes for improving the cultivated apple.Key words: apple, resistance Vf gene, BAC library, sequence-characterized amplified regions (SCARs), fiber fluorescence in situ hybridization (fiber-FISH), positional cloning.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    URL: Article
    DOI: 10.1139/g01-105
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2001
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