In:
Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 40, No. 9 ( 1994-09-01), p. 730-735
Abstract:
The effect of the Ca 2+ antagonist flunarizine on Mucor rouxii germlings was analyzed. In sporangiospores cultivated in aerobiosis, the drug blocked germ tube emergence, but enlarged spheres continued to grow isodiametrically. The formation of broad protuberances and irregular buds was observed. Calcofluor staining indicated aberrations on the surface of the flunarizine-treated cells. This was not a simple switch to the yeast form, since labelling with rhodarmine-tagged phalloidin revealed various ring-shaped structures of F-actin, indicating furrowing of the plasma membrane. Flunarizine apparently did not interfere with nuclear divisions or with the integrity of microtubules. In young germlings, flunarizine treatment induced cessation of tip growth and promoted septation associated with the rearrangement of F-actin. Apical growth was reestablished after either the drug was removed or an excess of Ca 2+ ions was added to the medium. The results suggest that tip-related arrangement of F-actin requires flunarizine-sensitive Ca 2+ entry.Key words: Mucor rouxii, F-actin, Ca 2+ antagonist, flunarizine, germination.
Type of Medium:
Online Resource
ISSN:
0008-4166
,
1480-3275
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
1994
detail.hit.zdb_id:
280534-0
detail.hit.zdb_id:
1481972-7
SSG:
12
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