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  • Canadian Science Publishing  (13)
  • Biology  (13)
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  • Biology  (13)
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  • 1
    Online Resource
    Online Resource
    Unraveling aerobic cultivable cellulolytic microorganisms within the gastrointestinal tract of sheep ( Ovis aries ) and their evaluation for cellulose biodegradation
    Canadian Science Publishing ; 2022
    In:  Canadian Journal of Microbiology Vol. 68, No. 4 ( 2022-04), p. 237-248
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 68, No. 4 ( 2022-04), p. 237-248
    Abstract: Anaerobic cellulolytic microbes in the gastrointestinal tract (GT) of ruminants have been well-documented; however, knowledge of aerobic microbes with cellulolytic activities in the ruminant GT is comparably limited. Here, we unraveled aerobic cultivable cellulolytic microbes in the GT of Ujimqin sheep (Ovis aries) and evaluated the cellulolytic potential of the promising isolates. Twenty-two strains were found to possess cellulose-degrading potential by Congo-red staining and phylogenetic analysis of the 16S rDNA/ITS sequence revealed that all strains belonged to nine genera, i.e., Bacillus, Streptomyces, Pseudomonas, Lactobacillus, Brachybacterium, Sanguibacter, Rhizobium, Fusarium, and Aspergillus. Strains with high cellulolytic activity were selected to further evaluate the activities of various enzymes in lignocellulosic alfalfa hay (Medicago sativa). Among them, isolate Bacillus subtilis RE2510 showed the highest potential for cellulose degradation, considering the high endoglucanase (0.1478 ± 0.0014 IU mL −1 ), exoglucanase (0.1735 ± 0.0012 IU mL −1 ), and β-glucosidase (0.3817 ± 0.0031 IU mL −1 ) after 10-day incubation with alfalfa hay. A significant destruction effect of the cellulose structure and the attachment of B. subtilis RE2510 to the hay were also revealed using a scanning electron microscope. This study expands our knowledge of aerobic cellulolytic isolates from the GT of sheep and highlights their potential application as a microbial additive in the aerobic process of cellulose bioconversion.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/cjm-2021-0338
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2022
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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    Online Resource
  • 2
    Online Resource
    Online Resource
    H-NS represses transcription of the flagellin gene lafA of lateral flagella in Vibrio parahaemolyticus
    Canadian Science Publishing ; 2018
    In:  Canadian Journal of Microbiology Vol. 64, No. 1 ( 2018-01), p. 69-74
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 64, No. 1 ( 2018-01), p. 69-74
    Abstract: Swarming motility is ultimately mediated by the proton-powered lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf genes is tightly regulated by a number of environmental conditions and regulatory factors. The nucleoid-associated DNA-binding protein H-NS is a small and abundant protein that is widely distributed in bacteria, and H-NS-like protein-dependent expression of laf genes has been identified in Vibrio cholerae and V. parahaemolyticus. The data presented here show that H-NS acts as a repressor of the swarming motility in V. parahaemolyticus. A single σ 28 -dependent promoter was detected for lafA encoding the flagellin of the lateral flagella, and its activity was directly repressed by H-NS. Thus, H-NS represses swarming motility by directly acting on lafA. Briefly, this work revealed a novel function for H-NS as a repressor of the expression of lafA and swarming motility in V. parahaemolyticus.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/cjm-2017-0315
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2018
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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    Online Resource
  • 3
    Online Resource
    Online Resource
    Identification of New Delhi metallo-β-lactamase gene (NDM-1) from a clinical isolate of Acinetobacter junii in China
    Canadian Science Publishing ; 2012
    In:  Canadian Journal of Microbiology Vol. 58, No. 1 ( 2012-01), p. 112-115
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 58, No. 1 ( 2012-01), p. 112-115
    Abstract: New Delhi metallo-β-lactamase-1 (NDM-1) is a novel type of metallo-β-lactamase (MBL) responsible for bacterial resistance to β-lactam antibiotics. Acinetobacter junii was previously shown to possess a MBL phenotype; however, the genes responsible for this phenotype were not identified. In this study, we reported the identification of NDM-1 gene in a clinical isolate of A. junii from a child patient in China, which was resistant to all β-lactams except aztreonam but sensitive to aminoglycosides and quinolones. The cloned NDM-1 gene contained an open reading frame of 813 bp and had a nucleotide sequence 99.9% identical (812/813) to reported NDM-1 genes carried by Acinetobacter baumannii , Enterococcus faecium , Escherichia coli , and Klebsiella pneumoniae . Recombinant NDM-1 protein was successfully expressed in E. coli BL21, and antibiotic sensitivities of the NDM-1-producing E. coli were largely similar to the A. junii 1454 isolate. The findings of this study raise attention to the emergence and spread of NDM-1-carrying bacteria in China.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/w11-112
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2012
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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    Online Resource
  • 4
    Online Resource
    Online Resource
    Effects of inoculation with organic-phosphorus-mineralizing bacteria on soybean ( Glycine max ) growth and indigenous bacterial community diversity
    Canadian Science Publishing ; 2017
    In:  Canadian Journal of Microbiology Vol. 63, No. 5 ( 2017-05), p. 392-401
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 63, No. 5 ( 2017-05), p. 392-401
    Abstract: Three different organic-phosphorus-mineralizing bacteria (OPMB) strains were inoculated to soil planted with soybean (Glycine max), and their effects on soybean growth and indigenous bacterial community diversity were investigated. Inoculation with Pseudomonas fluorescens Z4-1 and Brevibacillus agri L7-1 increased organic phosphorus degradation by 22% and 30%, respectively, compared with the control at the mature stage. Strains P. fluorescens Z4-1 and B. agri L7-1 significantly improved the soil alkaline phosphatase activity, average well color development, and the soybean root activity. Terminal restriction fragment length polymorphism analysis demonstrated that P. fluorescens Z4-1 and B. agri L7-1 could persist in the soil at relative abundances of 2.0%–6.4% throughout soybean growth. Thus, P. fluorescens Z4-1 and B. agri L7-1 could potentially be used in organic-phosphorus-mineralizing biofertilizers. OPMB inoculation altered the genetic structure of the soil bacterial communities but had no apparent influence on the carbon source utilization profiles of the soil bacterial communities. Principal components analysis showed that the changes in the carbon source utilization profiles of bacterial community depended mainly on the plant growth stages rather than inoculation with OPMB. The results help to understand the evolution of the soil bacterial community after OPMB inoculation.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/cjm-2016-0758
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2017
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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    Online Resource
  • 5
    Online Resource
    Online Resource
    Null mutation in sspA of Cronobacter sakazakii influences its tolerance to environmental stress
    Canadian Science Publishing ; 2021
    In:  Canadian Journal of Microbiology Vol. 67, No. 12 ( 2021-12), p. 902-918
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 67, No. 12 ( 2021-12), p. 902-918
    Abstract: Cronobacter sakazakii is a known foodborne opportunistic pathogen that can affect the intestinal health of infants. Despite undergoing complex manufacturing processes and low water concentration in the finished product, infant formula has been associated with Cronobacter infections, suggesting that the pathogenicity of C. sakazakii may be related to its tolerance to stress. In this study, the effect of the stringent starvation protein A (SspA), which plays an important role in E. coli cellular survival under environmental stress, on the stress tolerance of C. sakazakii BAA894 was investigated by creating a sspA-knockout mutant. The effects of this mutation on acid, desiccation, and drug tolerance were assessed, and the results showed that acid tolerance decreased, while desiccation tolerance increased in LB and decreased in M9. Moreover, the minimum inhibitory concentrations of 10 antibiotics in LB medium and 8 antibiotics in M9 medium were determined and compared between the wild type and ΔsspA strains. Transcriptome analysis showed that 27.21% or 37.78% of the genes in ΔsspA were significantly differentially expressed in LB or M9 media, the genes relevant to microbial metabolism in diverse environments, and bacterial chemotaxis were analyzed in detail. The current study contributes to an improved understanding of the role of SspA in C. sakazakii BAA894 stress tolerance.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/cjm-2021-0114
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2021
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    The effects of Pantoea sp. strain Y4-4 on alfalfa in the remediation of heavy-metal-contaminated soil, and auxiliary impacts of plant residues on the remediation of saline–alkali soils
    Canadian Science Publishing ; 2017
    In:  Canadian Journal of Microbiology Vol. 63, No. 4 ( 2017-04), p. 278-286
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 63, No. 4 ( 2017-04), p. 278-286
    Abstract: The plant-growth-promoting rhizobacterium (PGPR) Y4-4 was isolated from plant rhizosphere soil and identified as Pantoea sp. by 16S rRNA sequence analysis. The effects of strain Y4-4 on alfalfa grown in heavy-metals-contaminated soil was investigated using a pot experiment. In a Cu-rich environment, the shoot dry mass and total dry mass of plants inoculated with strain Y4-4 increased by 22.6% and 21%, and Cu accumulation increased by 15%. In a Pb–Zn-rich environment, the shoot dry mass and total dry mass of plants inoculated with strain Y4-4 increased by 23.4% and 22%, and Zn accumulation increased by 30.3%. In addition, the salt tolerance and biomass of wheat seedlings could be improved by applying strain Y4-4 mixed with plant residue as a result of the Cu-rich plant residues providing copper nutrition to wheat. This study offers an efficient PGPR with strong salt tolerance and a safe strategy for the post-treatment of plant residue.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/cjm-2016-0369
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2017
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    An important role for secreted esterase in disease establishment of the wheat powdery mildew fungus Blumeria graminis f. sp. tritici
    Canadian Science Publishing ; 2011
    In:  Canadian Journal of Microbiology Vol. 57, No. 3 ( 2011-03), p. 211-216
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 57, No. 3 ( 2011-03), p. 211-216
    Abstract: The activity of esterase secreted by conidia of wheat powdery mildew fungus, Blumeria graminis f. sp. tritici, was assayed using indoxyl acetate hydrolysis, which generates indigo blue crystals. Mature, ungerminated, and germinating conidia secrete esterase(s) on artificial media and on plant leaf surfaces. The activity of these esterases was inhibited by diisopropyl fluorophosphate, which is selective for serine esterases. When conidia were inoculated on wheat leaves pretreated with diisopropyl fluorophosphate, both appressorial germ tube differentiation and symptom development were significantly impaired, indicating an important role of secreted serine esterases in wheat powdery mildew disease establishment.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/W10-120
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2011
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Effect of common bean seed exudates on growth, lipopolysaccharide production, and lipopolysaccharide transport gene expression of Rhizobium anhuiense
    Canadian Science Publishing ; 2020
    In:  Canadian Journal of Microbiology Vol. 66, No. 3 ( 2020-03), p. 186-193
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 66, No. 3 ( 2020-03), p. 186-193
    Abstract: Lipopolysaccharide (LPS) is essential for successful nodulation during the symbiosis of rhizobia and legumes. However, the detailed mechanism of the LPS in this process has not yet been clearly elucidated. In this study, the effects of common bean seed exudates on the growth, lipopolysaccharide production, and lipopolysaccharide transport genes expression (lpt) of Rhizobium anhuiense were investigated. Rhizobium anhuiense exposed to exudates showed changes in LPS electrophoretic profiles and content, whereby the LPS band was wider and the LPS content was higher in R. anhuiense treated with seed exudates. Exudates enhanced cell growth of R. anhuiense in a concentration-dependent manner; R. anhuiense exposed to higher doses of the exudate showed faster growth. Seven lpt genes of R. anhuiense were amplified and sequenced. Sequences of six lpt genes, except for lptE, were the same as those found in previously analyzed R. anhuiense strains, while lptE shared low sequence similarity with other strains. Exposure to the exudates strongly stimulated the expression of all lpt genes. Approximately 6.7- (lptG) to 301-fold (lptE) increases in the transcriptional levels were observed after only 15 min of exposure to exudates. These results indicate that seed exudates affect the LPS by making the cell wall structure more conducive to symbiotic nodulation.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/cjm-2019-0413
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2020
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Effects of copper on the composition and diversity of microbial communities in laboratory-scale swine manure composting
    Canadian Science Publishing ; 2018
    In:  Canadian Journal of Microbiology Vol. 64, No. 6 ( 2018-06), p. 409-419
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 64, No. 6 ( 2018-06), p. 409-419
    Abstract: This study investigated the effects of adding copper at 3 treatment levels (0 (control: CK), 200 (low: L), and 2000 (high: H) mg·kg −1 treatments) on the bacterial communities during swine manure composting. The abundances of the bacteria were determined by quantitative PCR and their compositions were evaluated by high-throughput sequencing. The results showed that the abundance of bacteria was inhibited by the H treatment during days 7–35, and principal component analysis clearly separated the H treatment from the CK and L treatments. Actinobacteria, Firmicutes, and Proteobacteria were the dominant bacterial taxa, and a high copper concentration decreased the abundances of bacteria that degrade cellulose and lignin (e.g., class Bacilli and genus Truepera), especially in the mesophilic and thermophilic phases. Moreover, network analysis showed that copper might alter the co-occurrence patterns of bacterial communities by changing the properties of the networks and the keystone taxa, and increase the competition by increasing negative associations between bacteria during composting. Temperature, water-soluble carbohydrates, and copper significantly affected the variations in the bacterial community according to redundancy analysis. The copper content mainly contributed to the bacterial community in the thermophilic and cooling phases, where it had positive relationships with potentially pathogenic bacteria (e.g., Corynebacterium_1 and Acinetobacter).
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/cjm-2017-0622
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2018
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Short hairpin RNA-mediated inhibition of measles virus replication in vitro
    Canadian Science Publishing ; 2010
    In:  Canadian Journal of Microbiology Vol. 56, No. 1 ( 2010-01), p. 77-80
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 56, No. 1 ( 2010-01), p. 77-80
    Abstract: Rab9 has been identified as a key component for the replication of measles virus (MV). In this study, gene-specific shRNAs were developed to suppress the replication of MV in culture cells by silencing the expression of Rab9 GTPase gene. Rab9 GTPase gene-specific shRNAs were designed and cloned into the expression vector of pSUPER.neo+EGFP. Vero-E6 cells were transfected with the recombinant plasmid via liposome and then infected with MV. The expression of Rab9 GTPase mRNA and protein were assayed by RT-PCR and Western blotting, respectively. ShRNA-mediated inhibition of MV replication was further evaluated by detecting the titer of MV. The results showed that the expression of Rab9 GTPase was dramatically and stably downregulated by the generated shRNAs targeting Rab9 GTPase gene, which contribute to the inhibition of MV replication, indicating these shRNAs could be potentially developed into therapeutic agents for the treatment of MV infection in the future.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/W09-103
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2010
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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