In:
Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 46, No. 11 ( 2000-11-01), p. 1012-1020
Abstract:
In this study, we investigated the size and structure of autotrophic ammonia oxidizer (AAO) communities in the groundwater of a contamination plume originating from a mill-tailings disposal site. The site has high levels of dissolved N from anthropogenic sources, and exhibited wide variations in the concentrations of NO 3 - and NH 3 + NH 4 + . Community structures were examined by PCR-DGGE targeting 16S rDNA with band excision and sequence analysis, and by analysis of amoA fragment clone libraries. AAO population sizes were estimated by competitive PCR targeting the gene amoA, and correlated significantly with nitrate concentration. Most samples revealed novel diversity in AAO 16S rDNA and amoA gene sequences. Both 16S rDNA and amoA analyses suggested that all samples were dominated by Nitrosomonas sp., Nitrosospira sp. being detected in only 3 of 15 samples. This study indicated numerical dominance of Nitrosomonas over Nitrosospira in groundwater, and suggests that groundwater ammonia oxidizers are more similar to those dominating freshwater sediments than bulk soil.Key words: Nitrosomonas, Nitrosospira, nitrification, groundwater, amoA, 16S.
Type of Medium:
Online Resource
ISSN:
0008-4166
,
1480-3275
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
2000
detail.hit.zdb_id:
280534-0
detail.hit.zdb_id:
1481972-7
SSG:
12
Permalink