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  • Cambridge University Press (CUP)  (3)
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  • Cambridge University Press (CUP)  (3)
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  • 1
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    Online Resource
    Isolation, identification and expression profile of fourteen differentially expressed sequence tags in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs
    Cambridge University Press (CUP) ; 2007
    In:  Chinese Journal of Agricultural Biotechnology Vol. 4, No. 1 ( 2007-04), p. 9-14
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    In: Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 4, No. 1 ( 2007-04), p. 9-14
    Abstract: In order to detect the molecular mechanism of heterosis in pigs, an mRNA differential display (DD) technique was performed to investigate the differences in gene expression in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs. Fourteen expressed sequence tags (ESTs), differentially expressed between the hybrid and purebred pigs, were isolated and identified through semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Nucleotide sequence analysis revealed that the 14 ESTs are not homologous to any of the known genes or ESTs. These novel ESTs were then deposited in the GenBank database. Tissue expression profile analysis showed that the ESTs were expressed in most tissues, including heart, spleen, liver, kidney, small intestine, ovary and lung, and this also implied that these genes must be important for the life process. Our results indicate the diversity of differential display of genes between the hybrids and purebreds in the Meishan×Large White cross combination. Results also suggest that heterosis in pigs might be derived from the differential expression of many indispensable genes in specific life phases.
    Type of Medium: Online Resource
    ISSN: 1479-2362 , 1479-2370
    URL: Article
    DOI: 10.1017/S1479236207001209
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2007
    detail.hit.zdb_id: 2188055-4
    detail.hit.zdb_id: 2198867-5
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  • 2
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    Isolation, cloning and sequence analysis of differentially expressed genes in the backfat tissue between hybrids and parents in pigs
    Cambridge University Press (CUP) ; 2006
    In:  Chinese Journal of Agricultural Biotechnology Vol. 3, No. 1 ( 2006-04), p. 47-52
    Details
    In: Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 3, No. 1 ( 2006-04), p. 47-52
    Abstract: In order to reveal the molecular basis of heterosis, Large White (LW), an introduced European pig breed, and Meishan (MS), a Chinese indigenous pig breed, were selected to hybridize directly and reciprocally in the present experiment. mRNA differential display (DD) technique was performed to identify genes that were differentially expressed in the backfat tissues of hybrids (LW×MS, MS×LW) and purebred (LW×LW, MS×MS) pigs. The ten anchor primers in combination with ten arbitrary primers (100 sets in total) were used and nearly 1500 reproducible bands were observed in polyacrylamide gels. The 40 differentially displayed bands were selected for cloning and sequencing. Thirty-six out of 40 expressed sequence tags (ESTs) proved to be novel and the sequences were submitted to GenBank (accession No. CV507051-CV507087); the other four showed similarity to known genes published in GenBank. Three among 36 novel ESTs were chosen for further identification with semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). The result showed that two ESTs were differentially expressed, and the third showed no obvious difference between hybrids and purebreds. In order to reduce the percentage of false-positive DD, RNA pools of four types of pigs were constructed, by mixing samples from six pigs of the same genotype, and subjected to DD. Stringent annealing temperature was applied and only bands that could be repeated in duplicate PCR were used for further study. The results showed that the expression pattern of these 36 ESTs differed among the four genotypes of pigs, suggesting that the genes corresponding to these differentially expressed ESTs might be related to the heterosis occurring in fat tissue.
    Type of Medium: Online Resource
    ISSN: 1479-2362 , 1479-2370
    URL: Article
    DOI: 10.1079/CJB200592
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2006
    detail.hit.zdb_id: 2188055-4
    detail.hit.zdb_id: 2198867-5
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  • 3
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    The genetic evaluation of crossbred pig populations for heterosis research by microsatellite markers
    Cambridge University Press (CUP) ; 2006
    In:  Chinese Journal of Agricultural Biotechnology Vol. 3, No. 3 ( 2006-12), p. 243-248
    Details
    In: Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 3, No. 3 ( 2006-12), p. 243-248
    Abstract: The molecular genetic characteristics were evaluated, and the genetic effects of marker loci on heterosis of three traits (birth weight, BWT; average daily gain, ADG; and feed and meat ratio, FMR) were analysed in the experimental pig populations: Yorkshire (Y, n =34), Landrace (L, n =46) and Meishan (M, n =55); Yorkshire×Landrace (YL, n =32) and its reciprocal (LY, n =36), Yorkshire×Meishan (YM, n =82) and its reciprocal (MY, n =47), by 39 microsatellite markers selected from pig chromosomes SSC4, 6, 7, 8 and 13. The results indicated that observed alleles ranged from 2 to 6, average 4.13, observed heterozygosity varied from 0.39 (Y) to 0.58 (YM+MY) and average polymorphism information content (PIC) varied from 0.33 (Y) to 0.5 (YM). There were two loci ( sw2155 and sw1037 ) at which the alleles were fixed in Y and L, and there were three loci ( sw2409 , sw2454 and sw1691 ) at which the alleles were fixed in M. The results from heterozygosity and kinship analysis revealed an intrinsic genetic relationship among the seven populations. Furthermore, the results on genetic effect analysis indicate that several marker loci had a significant effect on heterosis of the three traits in the two different F 1 crossbred populations ( P ⩽0.01), for example s0161 , swr1130 and sw1856 for BWT, sw1856 and swr2036 for ADG, and sw1302 and swr2036 for FMR. The significant marker loci implied a deep genetic relationship between molecular marker loci and heterosis.
    Type of Medium: Online Resource
    ISSN: 1479-2362 , 1479-2370
    URL: Article
    DOI: 10.1079/CJB2006115
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2006
    detail.hit.zdb_id: 2188055-4
    detail.hit.zdb_id: 2198867-5
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