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  • Cambridge University Press (CUP)  (5)
  • 1
    In: British Journal of Nutrition, Cambridge University Press (CUP), Vol. 122, No. 11 ( 2019-12-14), p. 1303-1312
    Abstract: Consumption of sugar-sweetened beverages (SSB) by infants and young children are less explored in Asian populations. The Growing Up in Singapore Towards healthy Outcomes cohort study examined associations between SSB intake at 18 months and 5 years of age, with adiposity measures at 6 years of age. We studied Singaporean infants/children with SSB intake assessed by FFQ at 18 months of age ( n 555) and 5 years of age ( n 767). The median for SSB intakes is 28 (interquartile range 5·5–98) ml at 18 months of age and 111 (interquartile range 57–198) ml at 5 years of age. Association between SSB intake (100 ml/d increments and tertile categories) and adiposity measures (BMI standard deviation scores ( sd units), sum of skinfolds (SSF)) and overweight/obesity status were examined using multivariable linear and Poisson regression models, respectively. After adjusting for confounders and additionally for energy intake, SSB intake at age 18 months were not significantly associated with later adiposity measures and overweight/obesity outcomes. In contrast, at age 5 years, SSB intake when modelled as 100 ml/d increments were associated with higher BMI by 0·09 (95 % CI 0·02, 0·16) sd units, higher SSF thickness by 0·68 (95 % CI 0·06, 1·44) mm and increased risk of overweight/obesity by 1·2 (95 % CI 1·07, 1·23) times at age 6 years. Trends were consistent with SSB intake modelled as categorical tertiles. In summary, SSB intake in young childhood is associated with higher risks of adiposity and overweight/obesity. Public health policies working to reduce SSB consumption need to focus on prevention programmes targeted at young children.
    Type of Medium: Online Resource
    ISSN: 0007-1145 , 1475-2662
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2019
    detail.hit.zdb_id: 2016047-1
    SSG: 12
    SSG: 21
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  • 2
    Online Resource
    Online Resource
    Cambridge University Press (CUP) ; 2009
    In:  Chinese Journal of Agricultural Biotechnology Vol. 6, No. 1 ( 2009-04), p. 1-9
    In: Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 6, No. 1 ( 2009-04), p. 1-9
    Abstract: The techniques used to detect genetically modified organisms (GMO), including qualitative polymerase chain reaction (PCR), quantitative PCR, enzyme-linked immunosorbent assay (ELISA) and many others, are systematically described and discussed. The application progress of GMO in species-specific detection, endogenous genes, standard substances and restraining factors influencing detection are reviewed. The ongoing problems and development prospects of detection techniques of GMO are also pointed out.
    Type of Medium: Online Resource
    ISSN: 1479-2362 , 1479-2370
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2009
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  • 3
    Online Resource
    Online Resource
    Cambridge University Press (CUP) ; 2007
    In:  Chinese Journal of Agricultural Biotechnology Vol. 4, No. 1 ( 2007-04), p. 15-19
    In: Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 4, No. 1 ( 2007-04), p. 15-19
    Abstract: A rapid and accurate real-time quantitative polymerase chain reaction (real-time PCR) method with SYBR Green I was established for detecting Escherichia coli O157:H7. A pair of primers were designed to amplify the eae gene. The dissociation curves showed that the amplification product was very specific. The optimal conditions and standard curve were established. The result indicated that real-time PCR was 1000 times more sensitive than ordinary PCR.
    Type of Medium: Online Resource
    ISSN: 1479-2362 , 1479-2370
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2007
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  • 4
    Online Resource
    Online Resource
    Cambridge University Press (CUP) ; 2007
    In:  Acta Neuropsychiatrica Vol. 19, No. 6 ( 2007-12), p. 344-350
    In: Acta Neuropsychiatrica, Cambridge University Press (CUP), Vol. 19, No. 6 ( 2007-12), p. 344-350
    Abstract: The Ser9Gly polymorphism in dopamine D3 receptor gene ( DRD3 ) was considered an important factor in the pathogenesis of schizophrenia. Allele and genotype frequencies of this polymorphism were studied in different ethnic groups of schizophrenic patients. However, the results have been inconclusive. Objective: To determine whether the DRD3 Ser9Gly polymorphism is associated with schizophrenia or influences its psychopathological symptoms in Han Chinese population. Method: We recruited 256 schizophrenic patients and 285 normal controls matched for gender, age and ethnicity. Pretreatment psychotic symptoms were evaluated with the Positive and Negative Symptom Scale (PANSS) in 128 acutely exacerbated schizophrenic in-patients. Genotyping of Ser9Gly polymorphism was performed with a polymerase chain reaction restriction fragment length polymorphism method and reconfirmed by a direct sequencing technique. Results: No significant difference was found between either patients with schizophrenia or with more homogeneous schizophrenic subgroups and healthy controls in genotype distributions and allele frequencies for the DRD3 Ser9Gly polymorphism. Similarly, DRD3 Ser9Gly genotype differences failed to reach significance in PANSS global, positive, negative and general symptoms scores. There is a trend ( P = 0.064) towards higher PANSS positive symptoms scores in subjects carrying the Gly/Gly genotype. Conclusion: This study does not support the role of DRD3 Ser9Gly polymorphism in increasing genetic risk for schizophrenia in Han Chinese population. Still, there is a possibility that the DRD3 Ser9Gly variant may reflect genetic variation of severity of positive symptoms in acutely exacerbated schizophrenia. Further studies are warranted to investigate the effect of the DRD3 Ser9Gly polymorphism in relation to longer time course of schizophrenia, including treatment response to antipsychotics.
    Type of Medium: Online Resource
    ISSN: 0924-2708 , 1601-5215
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2007
    detail.hit.zdb_id: 2077830-2
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  • 5
    Online Resource
    Online Resource
    Cambridge University Press (CUP) ; 2022
    In:  Disaster Medicine and Public Health Preparedness Vol. 16, No. 3 ( 2022-06), p. 949-955
    In: Disaster Medicine and Public Health Preparedness, Cambridge University Press (CUP), Vol. 16, No. 3 ( 2022-06), p. 949-955
    Abstract: The aim of this study was to investigate risk factors and psychological stress of health-care workers (HCWs) with coronavirus disease 2019 (COVID-19) in a nonfrontline clinical department. Methods: Data of 2 source patients and all HCWs with infection risk were obtained in a department in Wuhan from January to February 2020. A questionnaire was designed to evaluate psychological stress of COVID-19 on HCWs. Results: The overall infection rate was 4.8% in HCWs. Ten of 25 HCWs who contacted with 2 source patients were diagnosed with confirmed COVID-19 (8/10) and suspected COVID-19 (2/10). Other 2 HCWs were transmitted by other patients or colleagues. Close care behaviors included physical examination (6/12), life nursing (4/12), ward rounds (4/12), endoscopic examination (2/12). Contacts fluctuated from 1 to 24 times and each contact was short (8.1 min ± 5.6 min). HCWs wore surgical masks (11/12), gloves (7/12), and isolation clothing (3/12) when providing medical care. Most HCWs experienced a mild course with 2 asymptomatic infections, taking 9.8 d and 20.9 d to obtain viral shedding and clinical cure, respectively. Psychological stress included worry (58.3%), anxiety (83.3%), depression (58.3%), and insomnia (58.3%). Conclusions: Close contact with COVID-19 patients and insufficient protection were key risk factors. Precaution measures and psychological support on COVID-19 is urgently required for HCWs.
    Type of Medium: Online Resource
    ISSN: 1935-7893 , 1938-744X
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2022
    detail.hit.zdb_id: 2375268-3
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