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  • 1
    Online Resource
    Online Resource
    Expression and cellular distribution of zona pellucida glycoproteins in canine oocytes before and after in vitro maturation
    Cambridge University Press (CUP) ; 2015
    In:  Zygote Vol. 23, No. 6 ( 2015-12), p. 863-873
    Details
    In: Zygote, Cambridge University Press (CUP), Vol. 23, No. 6 ( 2015-12), p. 863-873
    Abstract: This study was aimed at investigating zona pellucida glycoproteins (ZP) ZP2, ZP3 mRNA expression as well as ZP3, ZP4 (ZPB) protein distribution before and after in vitro maturation (IVM) in canine oocytes. The cumulus–oocyte complexes (COCs) were recovered from 27 anoestrous mongrel bitches and matured for 72 h in TCM199 medium. The canine COCs were analysed before and after IVM. Using real-time quantitative polymerase chain reaction (RQ-PCR), both groups of oocytes were analysed for detection of ZP2 and ZP3 mRNA profiles as well as using confocal microscopic analysis for observation of ZP3 and ZP4 protein distribution. In post-IVM canine oocytes an increase in transcript content of ZP2 and ZP3 genes as well as a decrease in ZP3 and ZP4 protein levels were observed when compared with pre-IVM oocytes. Moreover, the ZP4 protein before IVM was significantly distributed in the peripheral area of cytoplasm, whereas after IVM it was localized rather than in the entire cytoplasm. In contrast, the ZP3 protein was found both before and after IVM was distributed in the peripheral area of the cytoplasm. In conclusion, we suggest that the expression of ZP2 and ZP3 genes is associated with the maturation stage of canine oocytes, as higher mRNAs levels were found after IVM. However, a decreased expression of ZP3 and ZP4 proteins after IVM suggests maturation-dependent down-regulation of these protein translations, which may result in disturbed fertilization.
    Type of Medium: Online Resource
    ISSN: 0967-1994 , 1469-8730
    URL: Article
    DOI: 10.1017/S0967199414000549
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2015
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  • 2
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    Online Resource
    Targeting Glioblastoma Invasion with GSK-3 inhibitors: Rapid Effects on the EMT Marker Vimentin
    Cambridge University Press (CUP) ; 2014
    In:  Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques Vol. 41, No. s2 ( 2014-10), p. S1-S2
    Details
    In: Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques, Cambridge University Press (CUP), Vol. 41, No. s2 ( 2014-10), p. S1-S2
    Type of Medium: Online Resource
    ISSN: 0317-1671 , 2057-0155
    URL: Article
    DOI: 10.1017/cjn.2014.47
    RVK:
    XA 10000
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2014
    detail.hit.zdb_id: 2577275-2
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  • 3
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    Expression of INHβA and INHβB proteins in porcine oocytes cultured in vitro is dependent on the follicle size
    Cambridge University Press (CUP) ; 2015
    In:  Zygote Vol. 23, No. 2 ( 2015-04), p. 205-211
    Details
    In: Zygote, Cambridge University Press (CUP), Vol. 23, No. 2 ( 2015-04), p. 205-211
    Abstract: The current study aimed to investigate differential expression of inhibin βA (INHβA) and inhibin βB (INHβB) in porcine oocytes before or after in vitro maturation (IVM) isolated from follicles of various sizes. Porcine oocytes isolated from large, medium and small follicles (40 from each) were used to study the INHβA and INHβB protein expression pattern using western blot analysis before or after 44 h of oocyte IVM. An increased expression of INHβA was found in oocytes collected from large and medium follicles compared with small follicles before or after IVM ( P 〈 0.001, P 〈 0.05, respectively). Similarly, higher INHβB levels were observed in oocytes recovered from large follicles compared with small ( P 〈 0.01). As INHβA and INHβB are expressed in both porcine follicular somatic cells and oocytes, it can be assumed that these transforming growth factor beta (TGFβ) superfamily factors are involved in the regulation of molecular bi-directional pathways during follicle and oocyte development, and can be recognized as markers of follicle and oocyte maturation. Moreover, the current study clearly demonstrated that inhibin expression is substantially associated with porcine follicle growth and development.
    Type of Medium: Online Resource
    ISSN: 0967-1994 , 1469-8730
    URL: Article
    DOI: 10.1017/S0967199413000439
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2015
    detail.hit.zdb_id: 1483381-5
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  • 4
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    Expression and cellular distribution of estrogen and progesterone receptors and the real-time proliferation of porcine cumulus cells
    Cambridge University Press (CUP) ; 2015
    In:  Zygote Vol. 23, No. 6 ( 2015-12), p. 836-845
    Details
    In: Zygote, Cambridge University Press (CUP), Vol. 23, No. 6 ( 2015-12), p. 836-845
    Abstract: Although the expression of estrogen and progesterone receptors within porcine ovary and cumulus–oocyte complexes (COCs) is well recognized, still little information is known regarding expression of the progesterone receptor (PGR), PGR membrane component 1 (PGRMC1) and of estrogen-related receptors (ERRγ and ERRβ/γ) in separated cumulus cells in relation to real-time proliferation. In this study, a model of oocytes-separated cumulus cells was used to analyze the cell proliferation index and the expression PGR, PGRMC1 and of ERRγ and ERRβ/γ during 96-h cultivation in vitro using real-time quantitative PCR (qRT-PCR) and confocal microscopic observation. We found that PGR protein expression was increased at 0 h, compared with PGR protein expression after 96 h of culture ( P 〈 0.001). The expression of PGRMC1, ERRγ and ERRβ/γ was unchanged. After using qRT-PCR we did not found statistical differences in expression of PGR, PGRMC1, ERRγ and ERRβ/γ during 96 h of cumulus cells in vitro culture (IVC). We supposed that the differential expression of the PGR protein at 0 h and after 96 h is related to a time-dependent down-regulation, which may activate a negative feedback. The distribution of PGR, PGRMC1 proteins may be linked with the translocation of receptors to the cytoplasm after the membrane binding of respective agonists and intra-cytoplasmic signal transduction. Furthermore, cumulus cells analyzed at 0 h were characterized by decreased proliferation index, whereas those after 96 h of culture revealed a significant increase of proliferation index, which may be associated with differentiation/luteinization of these cells during real-time proliferation.
    Type of Medium: Online Resource
    ISSN: 0967-1994 , 1469-8730
    URL: Article
    DOI: 10.1017/S0967199414000495
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2015
    detail.hit.zdb_id: 1483381-5
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  • 5
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    Online Resource
    Effect of the physical activity program on the treatment of resistant hypertension in primary care
    Cambridge University Press (CUP) ; 2018
    In:  Primary Health Care Research & Development Vol. 19, No. 6 ( 2018-11), p. 575-583
    Details
    In: Primary Health Care Research & Development, Cambridge University Press (CUP), Vol. 19, No. 6 ( 2018-11), p. 575-583
    Abstract: Regular physical activity is widely recommended for patients with arterial hypertension as an essential component of lifestyle modification. Much less is known about the impact of physical exercise on the management of treatment of resistant hypertension (RH). The aim was to assess the effect of physical activity program intensified by mobile phone text reminders on blood pressure control in subjects with RH managed in the primary care. Methods In total, 53 patients with primary hypertension were qualified, including 27 who met the criteria for RH and 26 with well-controlled hypertension (WCH). Ambulatory 24-h blood pressure was monitored and body composition evaluated with bioimpedance and habitual physical activity profile was determined continuously over 72 h with accelerometer. All measurements were performed at baseline and after three and six months. The patients were asked to modify their lifestyle according to American Heart Association Guidelines that included regular aerobic physical activity tailored to individual needs. Findings Physical activity in RH increased significantly after six months compared with control subjects ( P =0.001). Office systolic blood pressure (SBP) and diastolic blood pressure (DBP) in the RH group decreased significantly after three months but after six months only office DBP remained significantly lower. After three months 24-h SBP decreased by 3.1±11 mmHg ( P =0.08) and DBP by 2.0±6 mmHg ( P =0.17) in RH, whereas in WCH respective changes were +1.2±10 and −0.3±6 mmHg. After six months 24-h BP changes were similar. Conclusion Individualized structured physical activity program increases physical activity in the treatment of resistant hypertensives in primary care but the effect on 24-h blood pressure is only transient.
    Type of Medium: Online Resource
    ISSN: 1463-4236 , 1477-1128
    URL: Article
    DOI: 10.1017/S1463423618000154
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2018
    detail.hit.zdb_id: 2027892-5
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  • 6
    Online Resource
    Online Resource
    Morphogenesis-related gene-expression profile in porcine oocytes before and after in vitro maturation
    Cambridge University Press (CUP) ; 2017
    In:  Zygote Vol. 25, No. 3 ( 2017-06), p. 331-340
    Details
    In: Zygote, Cambridge University Press (CUP), Vol. 25, No. 3 ( 2017-06), p. 331-340
    Abstract: Mammalian oocyte maturation is achieved when oocytes reach metaphase II (MII) stage, and accumulate mRNA and proteins in the cytoplasm following fertilization. It has been shown that oocytes investigated before and after in vitro maturation (IVM) differ significantly in transcriptomic and proteomic profiles. Additionally, folliculogenesis and oogenesis is accompanied by morphogenetic changes, which significantly influence further zygote formation and embryo growth. This study aimed to determine new transcriptomic markers of porcine oocyte morphogenesis that are associated with cell maturation competence. An Affymetrix microarray assay was performed on an RNA template isolated from porcine oocytes before ( n = 150) and after ( n = 150) IVM. The brilliant cresyl blue (BCB) staining test was used for identification of cells with the highest developmental capacity. DAVID (Database for Annotation, Visualization, and Integrated Discovery) software was used for the extraction of the genes belonging to a cell morphogenesis Gene Ontology group. The control group consisted of freshly isolated oocytes. In total, 12,000 different transcripts were analysed, from which 379 genes were downregulated and 40 were upregulated in oocytes following IVM. We found five genes, SOX9 , MAP1B , DAB2 , FN1 , and CXCL12 , that were significantly upregulated in oocytes after IVM ( in vitro group) compared with oocytes analysed before IVM ( in vivo group). In conclusion, we found new transcriptomic markers of oocyte morphogenesis, which may be also recognized as significant mediators of cellular maturation capacity in pigs. Genes SOX9 , MAP1B , DAB2 , FN1 , and CXCL12 may be involved in the regulation of the MII stage oocyte formation and several other processes that are crucial for porcine reproductive competence.
    Type of Medium: Online Resource
    ISSN: 0967-1994 , 1469-8730
    URL: Article
    DOI: 10.1017/S096719941700020X
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2017
    detail.hit.zdb_id: 1483381-5
    SSG: 12
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