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  • Cambridge University Press (CUP)  (6)
  • Biology  (6)
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  • Cambridge University Press (CUP)  (6)
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  • Biology  (6)
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  • 1
    Online Resource
    Online Resource
    A multiplex PCR for the identification of Echinococcus multilocularis , E. granulosus sensu stricto and E. canadensis that infect human
    Cambridge University Press (CUP) ; 2019
    In:  Parasitology Vol. 146, No. 12 ( 2019-10), p. 1595-1601
    Details
    In: Parasitology, Cambridge University Press (CUP), Vol. 146, No. 12 ( 2019-10), p. 1595-1601
    Abstract: Echinococcus granulosus sensu stricto ( s.s. ), Echinococcus multilocularis and Echinococcus canadensis are the common causes of human echinococcosis in China. An accurate species identification tool for human echinococcosis is needed as the treatments and prognosis are different among species. The present work demonstrates a method for the simultaneous detection of these three Echinococcus species based on multiplex polymerase chain reaction (mPCR). Specific primers of this mPCR were designed based on the mitochondrial genes and determined by extensive tests. The method can successfully detect either separated or mixed target species, and generate expected amplicons of distinct size for each species. Sensitivity of the method was tested by serially diluted DNA, showing a detection threshold as less as 0.32 pg for both E. granulosus s.s. and E. canadensis , and 1.6 pg for E. multilocularis . Specificity assessed against 18 other parasites was found to be 100% except weakly cross-react with E. shiquicus . The assay was additionally applied to 69 echinococcosis patients and 38 healthy persons, confirming the high reliability of the method. Thus, the mPCR described here has high application potential for clinical identification purposes, and can further provide a useful tool for evaluation of serology and imaging method.
    Type of Medium: Online Resource
    ISSN: 0031-1820 , 1469-8161
    URL: Article
    DOI: 10.1017/S0031182019000921
    RVK:
    WA 15000
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2019
    detail.hit.zdb_id: 1491287-9
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Phylogeny of diplozoids in five genera of the subfamily Diplozoinae Palombi, 1949 as inferred from ITS-2 rDNA sequences
    Cambridge University Press (CUP) ; 2006
    In:  Parasitology Vol. 134, No. 5 ( 2006-05), p. 695-703
    Details
    In: Parasitology, Cambridge University Press (CUP), Vol. 134, No. 5 ( 2006-05), p. 695-703
    Abstract: The phylogenetic relationship of 5 genera, i.e. Diplozoon Nordmann, 1832, Paradiplozoon Achmerov, 1974, Inustiatus Khotenovsky, 1978, Sindiplozoon Khotenovsky, 1981, and Eudiplozoon Khotenovsky, 1985 in the subfamily Diplozoinae Palombi, 1949 (Monogenea, Polyopisthocotylea) was inferred from rDNA ITS-2 region using neighbour-joining (NJ), maximum likelihood (ML) and Bayesian methods. The phylogenetic trees produced by using NJ, ML and Bayesian methods exhibit essentially the same topology. Surprisingly, freshwater species of Paradiplozoon from Europe clustered together with species of Diplozoon , but separated from Chinese Paradiplozoon species. The results of molecular phylogeny and lower level of divergence (4·1–15·7%) in ITS-2 rDNA among Paradiplozoon from Europe and Diplozoon and, on the other hand, high level of divergence (45·3–53·7%) among Paradiplozoon species from Europe and China might indicate the non-monophyletic origin of the genus Paradiplozoon . Also, the generic status of European Paradiplozoon needs to be revised. The species of Paradiplozoon in China is a basal group in Diplozoinae as revealed by NJ and Bayesian methods, and Sindiplozoon appears to be closely related to European Paradiplozoon and Diplozoon with their relationship to Eudiplozoon and Inustiatus being unresolved.
    Type of Medium: Online Resource
    ISSN: 0031-1820 , 1469-8161
    URL: Article
    DOI: 10.1017/S0031182006001971
    RVK:
    WA 15000
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2006
    detail.hit.zdb_id: 1491287-9
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
  • 3
    Online Resource
    Online Resource
    A new molecular nomenclature for Taenia hydatigena : mitochondrial DNA sequences reveal sufficient diversity suggesting the assignment of major haplotype divisions
    Cambridge University Press (CUP) ; 2021
    In:  Parasitology Vol. 148, No. 3 ( 2021-03), p. 311-326
    Details
    In: Parasitology, Cambridge University Press (CUP), Vol. 148, No. 3 ( 2021-03), p. 311-326
    Abstract: Cysticercosis caused by the metacestode larval stage of Taenia hydatigena formerly referred to as Cysticercus tenuicollis is a disease of veterinary importance that constitutes a significant threat to livestock production worldwide, especially in endemic regions due to condemnation of visceral organs and mortality rate of infected young animals. While the genetic diversity among parasites is found to be potentially useful in many areas of research including molecular diagnostics, epidemiology and control, that of T. hydatigena across the globe remains poorly understood. In this study, analysis of the mitochondrial DNA ( mt DNA) of adult worms and larval stages of T. hydatigena isolated from dogs, sheep and a wild boar in China showed that the population structure consists of two major haplogroups with very high nucleotide substitutions involving synonymous and non-synonymous changes. Compared with other cestodes such as Echinococcus spp., the genetic variation observed between the haplogroups is sufficient for the assignment of major haplotype or genotype division as both groups showed a total of 166 point-mutation differences between the 12 mitochondrial protein-coding gene sequences. Preliminary analysis of a nuclear protein-coding gene ( pepck ) did not reveal any peculiar changes between both groups which suggests that these variants may only differ in their mitochondrial makeup.
    Type of Medium: Online Resource
    ISSN: 0031-1820 , 1469-8161
    URL: Article
    DOI: 10.1017/S003118202000205X
    RVK:
    WA 15000
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2021
    detail.hit.zdb_id: 1491287-9
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
  • 4
    Online Resource
    Online Resource
    Genetic differentiation in populations of the cestode Bothriocephalus acheilognathi (Cestoda, Pseudophyllidea) as revealed by eight microsatellite markers
    Cambridge University Press (CUP) ; 2003
    In:  Parasitology Vol. 126, No. 5 ( 2003-05), p. 493-501
    Details
    In: Parasitology, Cambridge University Press (CUP), Vol. 126, No. 5 ( 2003-05), p. 493-501
    Abstract: The genetic structure of populations of the fish cestode, Bothriocephalus acheilognathi collected from Bailianhe Reservoir (BLH), Changshou (CSH) and Liangzi (LZH) Lakes was investigated by using 8 microsatellite loci. A total of 108 adult worms were genotyped at each of the 8 loci. For the 3 populations, the mean number of alleles per locus ranged from 2·38 to 5·5, and the mean expected heterozygosity ranged from 0·432 to 0·559. The average polymorphic information content (PIC) was from 0·384 to 0·492. The significant F is values indicated non-random mating within LZH and BLH populations. On the other hand, when samples were further classified into subpopulations at the level of host fish species, no or little heterozygote deficiency was detected at most loci, showing that cross-fertilization, predominantly, but not exclusively, must have occurred within the subpopulations. Microsatellite markers also revealed an unexpected high level of genetic differentiation, as measured by R st and N m values or by δ u 2 genetic distance among subpopulations from different hosts. Factors influencing the population genetic structure and the parasite host specificity are discussed.
    Type of Medium: Online Resource
    ISSN: 0031-1820 , 1469-8161
    URL: Article
    DOI: 10.1017/S003118200300297X
    RVK:
    WA 15000
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2003
    detail.hit.zdb_id: 1491287-9
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
  • 5
    Online Resource
    Online Resource
    Detection of Neospora caninum in aborted bovine fetuses and dam blood samples by nested PCR and ELISA and seroprevalence in Beijing and Tianjin, China
    Cambridge University Press (CUP) ; 2009
    In:  Parasitology Vol. 136, No. 11 ( 2009-09), p. 1251-1256
    Details
    In: Parasitology, Cambridge University Press (CUP), Vol. 136, No. 11 ( 2009-09), p. 1251-1256
    Abstract: Neospora caninum infection is a significant cause of abortion in cattle. We investigated the tissue distribution of N. caninum in aborted bovine fetuses and dam blood samples by a nested PCR assay, and compared the nested PCR with ELISA in the diagnosis of N. caninum infection. In total, 26 aborted fetuses and 813 blood samples were collected from 8 dairy herds in Beijing ( n =212) and Tianjin ( n =601), China. Fifteen fetuses (57·7%) were tested N. caninum- positive by the nested PCR. N. caninum DNA was detected from the brain of 52%, kidneys of 22%, skeletal muscle of 18%, and heart of 4% of the aborted fetuses. The PCR-positive cases (55%, 11/20) were higher than seropositive cows (40%, 8/20) in a subset of 20 fetuses, but the PCR results of blood samples of the 20 cows were all negative. The seroprevalence of the 813 samples was 15·5% (43·4% of samples from Beijing, 5·7% of samples from Tianjin), compared to the PCR-positive blood samples of 0·9%. Our study showed that the nested PCR is a valuable diagnostic tool for the primary diagnosis of N. caninum in aborted fetuses, while ELISA is the preferred assay for testing blood samples collected from cows. The two assays are complementary in determining whether abortions are associated with N. caninum infection in cattle.
    Type of Medium: Online Resource
    ISSN: 0031-1820 , 1469-8161
    URL: Article
    DOI: 10.1017/S0031182009990813
    RVK:
    WA 15000
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2009
    detail.hit.zdb_id: 1491287-9
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
  • 6
    Online Resource
    Online Resource
    Evaluation of salinomycin isolated from Streptomyces albus JSY-2 against the ciliate, Ichthyophthirius multifiliis
    Cambridge University Press (CUP) ; 2019
    In:  Parasitology Vol. 146, No. 4 ( 2019-04), p. 521-526
    Details
    In: Parasitology, Cambridge University Press (CUP), Vol. 146, No. 4 ( 2019-04), p. 521-526
    Abstract: The present study was undertaken to investigate the antiparasitic activity of extracellular products of Streptomyces albus . Bioactivity-guided isolation of chloroform extracts affording a compound showing potent activity. The structure of the compound was elucidated as salinomycin (SAL) by EI-MS, 1 H NMR and 13 C NMR. In vitro test showed that SAL has potent anti-parasitic efficacy against theronts of Ichthyophthirius multifiliis with 10 min, 1, 2, 3 and 4 h (effective concentration) EC 50 (95% confidence intervals) of 2.12 (2.22–2.02), 1.93 (1.98–1.88), 1.42 (1.47–1.37), 1.35 (1.41–1.31) and 1.11 (1.21–1.01) mg L −1 . In vitro antiparasitic assays revealed that SAL could be 100% effective against I. multifiliis encysted tomonts at a concentration of 8.0 mg L −1 . In vivo test demonstrated that the number of I. multifiliis trophonts on Erythroculter ilishaeformis treated with SAL was markedly lower than that of control group at 10 days after exposed to theronts ( P 〈 0.05). In the control group, 80% mortality was observed owing to heavy I. multifiliis infection at 10 days. On the other hand, only 30.0% mortality was recorded in the group treated with 8.0 mg L −1 SAL. The median lethal dose (LD 50 ) of SAL for E. ilishaeformis was 32.9 mg L −1 .
    Type of Medium: Online Resource
    ISSN: 0031-1820 , 1469-8161
    URL: Article
    DOI: 10.1017/S0031182018001919
    RVK:
    WA 15000
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2019
    detail.hit.zdb_id: 1491287-9
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
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