In:
Parasitology, Cambridge University Press (CUP), Vol. 143, No. 12 ( 2016-10), p. 1629-1638
Abstract:
Interleukin (IL)-13-associated signal pathway plays an important role in schistosomiasis hepatic fibrosis. In this study we tried to investigate the effects of corilagin to ameliorate schistosomiasis hepatic fibrosis through regulating IL-13-associated signal pathway in vitro and in vivo . Cellular model was set up with hepatic stellate cells-T6 cells stimulated by rIL-13 and male Balb/c mice were infected with Schistosoma japonicum cercariaeas as animal model. Liver histological changes were observed with haematoxylin and eosin staining. Masson staining was employed to observe the change of egg granulomas. Expression of Col (collagen) and Col III were examined with Immunohistochemistry. Western bolt was employed to detect the JAK-1 and IL13R α 1 proteins. The mRNA expression of Col I, Col III, IL-13, JAK-1 and IL13R α 1 were tested by quantitative polymerase chain reaction. As a result, less inflammatory changes were found in all corilagin groups compared with model group and praziquantel group. The mRNA levels of Col I, Col III, IL-13, JAK-1 and IL13R α 1 were significantly decreased after corilagin intervention ( P 〈 0·01). JAK-1 and IL-13R α 1 protein levels were also greatly decreased in the corilagin groups ( P 〈 0·01). In conclusion, corilagin could ameliorate schistosomiasis hepatic fibrosis by down-regulating the expression of IL-13 and signal molecules in IL-13 pathway.
Type of Medium:
Online Resource
ISSN:
0031-1820
,
1469-8161
DOI:
10.1017/S0031182016001128
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2016
detail.hit.zdb_id:
1491287-9
SSG:
12
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