GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • CSIRO Publishing  (4)
  • 1
    Online Resource
    Online Resource
    Xenografting restores spermatogenesis to cryptorchid testicular tissue but does not rescue the phenotype of idiopathic testicular degeneration in the horse (Equus caballus)
    CSIRO Publishing ; 2010
    In:  Reproduction, Fertility and Development Vol. 22, No. 4 ( 2010), p. 673-
    Details
    In: Reproduction, Fertility and Development, CSIRO Publishing, Vol. 22, No. 4 ( 2010), p. 673-
    Abstract: Spermatogenesis from many mammalian species occurs in fragments of normal testis tissue xenografted to mice. Here we apply xenografting to the study of testicular pathology. Using the horse model, we investigated whether exposure to a permissive extratesticular environment in the mouse host would rescue spermatogenesis in cryptorchid testicular tissue or in tissue affected by idiopathic testicular degeneration (ITD). In cryptorchid tissue, where the extratesticular environment is abnormal, xenografting induced spermatogenesis up to meiosis in a subpopulation of seminiferous tubules. Thus, spermatogonia survive and partially retain their potential to differentiate in cryptorchid horse testes. In contrast, the primary defect in equine ITD is hypothesised to be tissue autologous. In support of this, xenografting did not restore spermatogenesis to tissue affected by ITD, thus confirming that the testis itself is primarily diseased. This outcome was not affected by supplementation of exogenous gonadotropins to the mouse host or by reconstitution of a normal reproductive regulatory axis supplied by functional porcine testicular xenografts. These studies demonstrate the usefulness of xenografting for the study of testicular pathology.
    Type of Medium: Online Resource
    ISSN: 1031-3613
    URL: Article
    DOI: 10.1071/RD09014
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2010
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Freezability of boar spermatozoa is improved by exposure to 2-hydroxypropyl-beta-cyclodextrin
    CSIRO Publishing ; 2000
    In:  Reproduction, Fertility and Development Vol. 12, No. 4 ( 2000), p. 223-
    Details
    In: Reproduction, Fertility and Development, CSIRO Publishing, Vol. 12, No. 4 ( 2000), p. 223-
    Abstract: The influence of 2-hydroxypropyl-beta-cyclodextrin (HBCD) exposure on post-thaw spermatozoa prior to freezing using acrosome integrity and the parameters of motility was studied. Acrosomal status was monitored by means of FITC-labelled peanut agglutinin, and the motility parameters were assessed using a computer-assisted sperm motility analysis (CASA) system. The spermatozoa were exposed to HBCD over a period of 3 h, during which the cells were slowly cooled from 25 to 5˚C, and then frozen into pellets. The percentage of frozen–thawed spermatozoa with intact acrosomes in 40 mM HBCD group was approximately three-fold higher than that of the control. The motility and progressive motility values of the frozen–thawed spermatozoa were found to increase significantly with increased HBCD concentrations. On the other hand, further addition of cholesterol-3-sulfate to the BF5 extender containing 20 mM HBCD resulted in a drastic decrease in the percentage of spermatozoa with intact acrosomes, and decreased motility and progressive motility, suggesting that cholesterol-sulfate probably counter-acted the protective action of HBCD. In conclusion, the results of the present study indicate that HBCD protected boar spermatozoa against freeze–thaw damage, possibly by means of stimulating the efflux of membrane cholesterol.
    Type of Medium: Online Resource
    ISSN: 1031-3613
    URL: Article
    DOI: 10.1071/RD00058
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2000
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    THY1 is a surface marker of porcine gonocytes
    CSIRO Publishing ; 2014
    In:  Reproduction, Fertility and Development Vol. 26, No. 4 ( 2014), p. 533-
    Details
    In: Reproduction, Fertility and Development, CSIRO Publishing, Vol. 26, No. 4 ( 2014), p. 533-
    Abstract: Gonocytes are important for the study of spermatogenesis. Identification and isolation of gonocytes has been reported in rodents but not in pigs due to a lack of molecular markers for gonocytes. The objective of this study was to identify THY1 expression in porcine testicular tissue and subsequently utilise THY1 as a marker to isolate and enrich porcine gonocytes from testes of newborn piglets. Immunohistochemical analysis showed that THY1 was expressed in gonocytes. Double-immunofluorescent analysis of THY1 and ZBTB16 indicated that THY1 and ZBTB16 were partially co-localised in gonocytes. Double-immunofluorescent analysis of both THY1 and GATA4 suggested that THY1+ cells were not Sertoli cells. Magnetic-activated cell sorting of THY1+ cells yielded a cell population with an enrichment of UCHL1+ gonocytes 3.4-fold of that of the unsorted testicular cell population. Western blot and quantitative reverse transcription–polymerase chain reaction analyses confirmed that the selected THY1+ fraction had a higher expression of UCHL1 than the unsorted cells. In conclusion, the study demonstrated that THY1 is a surface marker of gonocytes in testes of pre-pubertal boars and could be utilised to identify and isolate porcine gonocytes. The findings will also facilitate culture and manipulation of male germline stem cells.
    Type of Medium: Online Resource
    ISSN: 1031-3613
    URL: Article
    DOI: 10.1071/RD13075
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2014
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Glutamine protects rabbit spermatozoa against oxidative stress via glutathione synthesis during cryopreservation
    CSIRO Publishing ; 2017
    In:  Reproduction, Fertility and Development Vol. 29, No. 11 ( 2017), p. 2183-
    Details
    In: Reproduction, Fertility and Development, CSIRO Publishing, Vol. 29, No. 11 ( 2017), p. 2183-
    Abstract: Mammalian spermatozoa are extremely susceptible to high doses of reactive oxygen species (ROS). The aim of the present study was to investigate the potential role of glutamine in protecting rabbit spermatozoa against ROS stress during cryopreservation and post-thaw incubation. Freshly ejaculated semen was diluted with Tris–citrate–glucose extender supplemented with glutamine. The addition of 20 mM glutamine significantly improved sperm motility, acrosome integrity, membrane integrity and mitochondrial activity. Meanwhile, 20 mM glutamine addition decreased lipid peroxidation and DNA damage in frozen–thawed spermatozoa. Interestingly, supplementation with 20 mM glutamine led to increases in glutathione content and γ-glutamyl cysteine synthetase and glutathione peroxidase activity, with concomitant decreases in ROS levels during cryopreservation and post-thaw incubation. In conclusion, the addition of glutamine to extender solutions protects rabbit spermatozoa from ROS attack by enhancing glutathione synthesis.
    Type of Medium: Online Resource
    ISSN: 1031-3613
    URL: Article
    DOI: 10.1071/RD17020
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2017
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...