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  • 1
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    Online Resource
    Molecular and morphological characterisation of Sphaeronema alni Turkina & Chizhov, 1986 (Nematoda: Sphaeronematidae) from Spain compared with a topotype population from Russia
    Brill ; 2010
    In:  Nematology Vol. 12, No. 4 ( 2010), p. 649-659
    Details
    In: Nematology, Brill, Vol. 12, No. 4 ( 2010), p. 649-659
    Abstract: The occurrence of a male-less population of Sphaeronema alni parasitising chestnut (Castanea sativa) roots and inducing a stelar syncytium is reported for the first time in Pola de Somiedo (Oviedo province), Spain. Morphometric and molecular characters of the Spanish population matched those of a topotype population from Russia. SEM observations showed swollen females having the first lip annulus wider than the second and appearing as a cap-like, circumoral elevation. The second-stage juveniles, having a single band in the lateral fields, were characterised by a non-annulated dome-shaped lip region derived from the fusion of the oral disc with all the lip sectors and lip annuli, and showing slit-like amphidial apertures and an oval prestoma. The sequences of the D2-D3 expansion segments of 28S rRNA, partial 18S rRNA and ITS rRNA gene for the Spanish and topotype populations of S. alni were congruent and matched those deposited in GenBank for another population from Germany, thereby confirming their conspecificity. A PCR-RFLP profile of D2-D3 of 28S rRNA for identification of this species was also provided. The phylogenetic relationships between S. alni populations and representatives of the suborder Criconematina, as inferred from analysis of partial 18S rRNA and D2-D3 of 28S gene sequences obtained in this and previous studies, indicated that S. alni formed a basal clade on the majority consensus Bayesian phylogenetic trees, standing together with Meloidoderita sp. or alone. These findings provide additional evidence of the need to clarify the position of Sphaeronema within Criconematina and its relationships with representatives of Tylenchulinae.
    Type of Medium: Online Resource
    ISSN: 1388-5545 , 1568-5411
    URL: Article
    DOI: 10.1163/138855410X489338
    Language: Unknown
    Publisher: Brill
    Publication Date: 2010
    detail.hit.zdb_id: 2019332-4
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Molecular variability and phylogeny of Schistonchus caprifici (Gasperrini, 1864) Cobb, 1927 (Nematoda: Aphelenchoididae) from Italy and Spain
    Brill ; 2010
    In:  Nematology Vol. 12, No. 4 ( 2010), p. 641-648
    Details
    In: Nematology, Brill, Vol. 12, No. 4 ( 2010), p. 641-648
    Abstract: Molecular characterisation of four Schistonchus caprifici populations (two from Spain and two from Italy), using the partial 18S, the D2-D3 expansion segments of 28S of ribosomal DNA (rDNA) and the partial mitochondrial gene for cytochrome c oxidase I (mtCOI), is provided. Amplicons from partial mtCOI were analysed using 'Single Strand Conformation Polymorphism' (SSCP) analysis in order to rapidly screen for genetic (haplotypic) variability. SSCP analysis of mtCOI revealed a close relationship between the Spanish and Italian populations with low intra-population variability and only two haplotypes were detected. Mitochondrial COI and ribosomal genes were analysed using Maximum Likelihood and Bayesian inference to reconstruct phylogenetic relationships within S. caprifici and other members of the family Aphelenchoididae. The phylogenetic analysis based on mtCOI showed no geographic variability among Italian, Spanish and Turkish populations. The results of phylogenetic analyses based on the D2-D3 expansion segments of 28S rDNA region and the partial 18S rDNA genes revealed a closer phylogenetic relationship between S. aureus, S. laevigatus, S. virens and S. centerae, whilst S. caprifici and S. guangzhouensis clustered separately. The phylogeny of the genus Schistonchus was well related to some morphological characters, such as position of excretory pore, presence/absence of labial disc and number and position of caudal papillae. The monophyly of the genus Schistonchus was rejected by the Shimodaira-Hasegawa test based on tree topologies.
    Type of Medium: Online Resource
    ISSN: 1388-5545 , 1568-5411
    URL: Article
    DOI: 10.1163/138855410X12628646276087
    Language: Unknown
    Publisher: Brill
    Publication Date: 2010
    detail.hit.zdb_id: 2019332-4
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Morphological and molecular characterisation of a new awl nematode, Dolichodorus mediterraneus sp. n. (Nematoda: Dolichodoridae), from Spain
    Brill ; 2007
    In:  Nematology Vol. 9, No. 2 ( 2007), p. 189-199
    Details
    In: Nematology, Brill, Vol. 9, No. 2 ( 2007), p. 189-199
    Abstract: A new species of awl nematode, Dolichodorus mediterraneus sp. n., is described from southern Spain. The new species is described and illustrated by light microscopy and scanning electron microscopy studies from specimens collected in a sandy soil around roots of cork oak (Quercus suber L.) from Hato Raton, Aznalcázar (Seville), southern Spain. Dolichodorus mediterraneus sp. n. is characterised by a prominent, rounded, lip region, which is offset from the body and has four to six very fine annuli; lateral fields areolated with three lines, ending posterior to anus level; tail 72-122 μm long, abruptly tapering to an acuminate, often spicate, terminus; tail projection 51-79 μ m long; spicules ventrally arcuate, 33-42 μm long; gubernaculum slightly ventrally curved, 14-22 μm long. Scanning electron microscopy observations showed a similar en face pattern to other Dolichodorus spp. Molecular data from the ribosomal large subunit D2-D3 expansion segment of 28S rDNA from paratypes yielded a single fragment of approximately 800 bp. This is the first record of the genus Dolichodorus in Spain and only the second in Europe. A key to species is provided.
    Type of Medium: Online Resource
    ISSN: 1388-5545 , 1568-5411
    URL: Article
    DOI: 10.1163/156854107780739108
    Language: Unknown
    Publisher: Brill
    Publication Date: 2007
    detail.hit.zdb_id: 2019332-4
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Seven new species of Trichodorus (Diphtherophorina, Trichodoridae) from Spain, an apparent centre of speciation
    Brill ; 2013
    In:  Nematology Vol. 15, No. 1 ( 2013), p. 57-100
    Details
    In: Nematology, Brill, Vol. 15, No. 1 ( 2013), p. 57-100
    Abstract: During a survey for Trichodoridae in cultivated and natural environments, mainly from southern Spain, 7 new Trichodorus species were found. Four of them, T. andalusicus n. sp., T. asturanus n. sp., T. silvestris n. sp. and T. parasilvestris n. sp., belong to the T. lusitanicus morpho-species group characterised in the male by the slightly ventrally curved spicules with a mid-blade constriction with bristles, and in the female by well developed, rounded triangular to quadrangular, vaginal sclerotised pieces. They were mainly differentiated based upon differences in body length, onchiostyle length, spicule shape and number of ventromedian cervical papillae in the male, and size and shape of the vaginal sclerotised pieces in the female. The presence of the T. lusitanicus morpho-species group with 8 species, apparently endemic to the Iberian Peninsula, could point to a recent speciation event. Additionally, 3 new species, T. onubensis n. sp., T. iliplaensis n. sp. and T. paragiennensis n. sp., more closely resembling the morpho-species group of T. sparsus , to which the Spanish species T. giennensis also belongs, were identified. They were differentiated based on body length, length of onchiostyle and position of the secretory-excretory pore and, in the male, by length and shape of the spicules and number of ventromedian cervical papillae and in the female by the vaginal sclerotised pieces. Molecular support to differentiate the new species using the D2-D3 expansion segments of 28S rDNA is also provided. In addition, a new population of T. variabilis from Greece was studied as well as T. lusitanicus type specimens, and a population of T. giennensis and T. lusitanicus were also sequenced.
    Type of Medium: Online Resource
    ISSN: 1388-5545 , 1568-5411
    URL: Article
    DOI: 10.1163/156854112X645598
    Language: Unknown
    Publisher: Brill
    Publication Date: 2013
    detail.hit.zdb_id: 2019332-4
    SSG: 12
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  • 5
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    Online Resource
    Comparative morphometrics and ribosomal DNA sequence analysis of Longidorus orientalis Loof, 1983 (Nematoda: Longidoridae) from Spain and Iran
    Brill ; 2010
    In:  Nematology Vol. 12, No. 4 ( 2010), p. 631-640
    Details
    In: Nematology, Brill, Vol. 12, No. 4 ( 2010), p. 631-640
    Abstract: During recent nematode surveys in a muddy soil around undetermined graminaceous plants in El Rocío, Huelva Province, in southern Spain, and from the rhizosphere of date palm associated with graminaceous vegetation from Abadan, in Khuzestan Province, south-west Iran, populations of Longidorus orientalis were identified. Morphological and morphometrical studies on these populations fit the original description and represent the first report from Spain and Europe. Molecular characterisation of L. orientalis from Spain and Iran, using D2-D3 expansion regions of 28S rRNA, 18S rRNA and ITS-rRNA, is provided. Sequences of the D2-D3 expansion regions and partial 18S genes were analysed using maximum likelihood and Bayesian inference to reconstruct phylogenetic relationships within L. orientalis and other Longidorus species. The results revealed a closer phylogenetic relationship with L. goodeyi for the D2-D3 expansion region and with L. vineacola for the partial 18S region.
    Type of Medium: Online Resource
    ISSN: 1388-5545 , 1568-5411
    URL: Article
    DOI: 10.1163/138855410X12628646276041
    Language: Unknown
    Publisher: Brill
    Publication Date: 2010
    detail.hit.zdb_id: 2019332-4
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Description and molecular characterisation of Paralongidorus litoralis sp. n. and P. paramaximus Heyns, 1965 (Nematoda: Longidoridae) from Spain
    Brill ; 2008
    In:  Nematology Vol. 10, No. 1 ( 2008), p. 87-101
    Details
    In: Nematology, Brill, Vol. 10, No. 1 ( 2008), p. 87-101
    Abstract: Paralongidorus litoralis sp. n., a new bisexual species of the genus, is described and illustrated by light microscopy, scanning electron microscopy and molecular studies from specimens collected in a coastal sand dune soil around roots of lentisc (Pistacia lentiscus L.) from Zahara de los Atunes (Cadiz), southern Spain. Paralongidorus litoralis sp. n. is characterised by the large body size (7.5-10.0 mm), a rounded lip region, clearly offset from the body by a collar-like constriction, and bearing a very large stirrup-shaped, amphidial fovea, with conspicuous slit-like aperture, a very long and flexible odontostyle ca 190 μm long, guiding ring located at 35 μm from anterior end, and males with spicules ca 70 μm long. In addition, identification data of a Spanish population of P. paramaximus Heyns, 1965 recovered from sandy soil of a commercial citrus orchard at Alcala de Guadaira (Seville), southern Spain, agree very well with the original description of the species from South Africa. The 18S rRNA and D2 and D3 expansion regions of 28S rRNA gene sequences were obtained for P. litoralis sp. n. and P. paramaximus. Phylogenetic analyses of P. litoralis sp. n. and P. paramaximus rRNA gene sequences and of Longidoridae sequences published in GenBank were done using maximum likelihood and Bayesian inference. In trees generated from the 18S data set Paralongidorus clustered as an external clade from Longidorus, and in trees generated from D2-D3 of 28S dataset Paralongidorus was monophyletic and nested within Longidorus. Maximum likelihood test supported the hypothesis of validity of the Paralongidorus genus.
    Type of Medium: Online Resource
    ISSN: 1388-5545 , 1568-5411
    URL: Article
    DOI: 10.1163/156854108783360186
    Language: Unknown
    Publisher: Brill
    Publication Date: 2008
    detail.hit.zdb_id: 2019332-4
    SSG: 12
    Location Call Number Limitation Availability
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