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  • 1
    ISSN: 1432-2048
    Keywords: Glycine ; Vacuolar H−+-ATP-ase ; Tonoplast (negative staining)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Highly purified tonoplast fractions isolated by preparative free-flow electrophoresis from hypocotyls of etiolated soybean (Glycine max L. (Merr.)) were examined by negative-staining electron microscopy, and many but not all vesicles were found to exhibit head and stalk structures resembling the 9-nm stalked F1 ATPase particles reported previously for Neurospora (Bowman et al., 1989, J. Biol. Chem. 264, 15606–15612). The structures show distinguishing characteristics similar to those for Neurospora. These include a cleft in the particle not exhibited by mitochondrial F1 ATPase and a tendency to disappear from the membrane when treated with nitrate plus Mg−2+-ATP-containing solutions. The position of the stalked ATPase structures, indicates that some of the tonoplast vesicles were oriented cytoplasmic side out whereas others were oriented cytoplasmic side in.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Free-flow electrophoresis ; Filipin ; Tonoplast ; Plasma Membrane ; Tonoplast ATPase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Preparative free-flow electrophoresis has been employed in combination with density gradient centrifugation to prepare fractions enriched in either tonoplast or plasma membrane from dark grown seedlings of cress (roots), zucchini (hypocotyls), soybean (hypocotyls) and maize (coleoptiles). A polyclonal antibody to the 72,000 Mr subunit to the maize tonoplast ATPase was used to identify the tonoplast fractions from the free-flow electrophoresic separations and to show the absence of tonoplast contamination in plasma membranes derived from the same homogenates. These findings confirm the identity of the tonoplast fraction based on the presence of the proton translocating ATPase determined previously from sucrose gradient fractionation and inhibitor studies to be a tonoplast marker. Using staining with phosphotungstic acid at low pH, the plasma membrane fractions obtained after free-flow electrophoresis were shown to be 〉 90% plasma membrane-derived with little or no cross-contamination of plasma membrane vesicles in the tonoplast-containing fractions. Finally, the composition of the fractions was correlated with the characteristic morphologic appearance after filipin treatment and freeze-fracture. By means of morphometric analyses using this criterion, both the identity and the purity of the tonoplast and the plasma membrane fractions received further confirmation. Essentially homogeneous fractions were obtained by subjecting fractions already enriched by a centrifugation method to the final separation by free-flow electrophoresis.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2018-11-19
    Description: The field of nutrition is facing numerous social, ecological and economic challenges in the coming decades. The food industry belongs to the most significant economic sectors worldwide and the increasing population of 9 billion in 2050 will cause a growing demand on food. So far, changing lifestyles, especially the global rising consumption of meat and dairy products are increasing environmental damage. Moreover our health and wellbeing are the direct result of healthy or unhealthy nourishment and influence follow-up indicators like individual and public health, the expense of the health sector and work productivity. The material footprint is a tool to measure and optimize the resource consumption of both products and their ingredients and the production processes along the whole value chain. It covers the whole life cycle of the products, from the extraction of raw materials to the processing industry, distribution, consumption, recycling, and disposal. In order to decrease resource consumption to a level in line with the planetary boundaries, the material footprint of household consumption should achieve a level of six to eight tonnes per capita in a year by 2050. This means a reduction in natural resource consumption by a factor of 5 to 10 in Western European countries. In order to ensure a decent lifestyle for all people in 2050, also the material footprint of nutrition has to be reduced significantly by 2050. The paper shows the relevance and role of nutrition in the overall material footprint of households on the basis of existing studies on the overall resource consumption caused by household consumption. Quantified meal and diet examples are given. It also discusses the causes of food waste and raises the question how a reduction of food waste is possible and can help decreasing the resource consumption in the food sector.
    Keywords: ddc:600
    Repository Name: Wuppertal Institut für Klima, Umwelt, Energie
    Language: English
    Type: conferenceobject , doc-type:conferenceObject
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