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  • Wiley-Blackwell  (40)
  • Blackwell Science Ltd  (6)
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  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Electrophysiological studies of photoreceptors from the horseshoe crab Limulus polyphemus continue to provide fundamental new knowledge of the photoresponse in invertebrates. Therefore, it is of particular interest to characterize the molecular components of the photoresponse in this system. Here we describe an arrestin cloned from a cDNA library constructed using poly(A)+ RNA isolated from Limulus lateral eyes. The protein, deduced from the arrestin cDNA, is most similar to arrestin from locust antennae (56% identity) and Drosophila phosrestin I (53% identity). Limulus arrestin was expressed in a heterologous system, and its properties were compared with those of a 46-kDa light-regulated phosprotein (pp46A) in Limulus photoreceptors described in previous studies from this laboratory. Arrestin and pp46A (a) have the same apparent molecular weight on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, (b) have an isoelectric point in the basic pH range, (c) require calmodulin and elevated Ca2+ levels for phosphorylation, (d) are immunoreactive with monoclonal antibody C10C10 directed against a sequence in bovine arrestin (S-antigen) that is perfectly conserved in the deduced arrestin protein, and (e) are associated with photoreceptors. We conclude that the arrestin described here and pp46A are the same protein. The results of this and previous studies show that in Limulus photoreceptors, light regulates the phosphorylation of arrestin in complex ways.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Global change biology 6 (2000), S. 0 
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Changes in soil organic carbon (SOC) in agricultural soils influence soil quality and greenhouse gas concentrations in the atmosphere. Land use, management practices, soil characteristics, and climate influence such changes. Using the Century model we estimated the rate of SOC change in agricultural soils of Canada for the period 1970 to 2010. This estimation was based on the estimated SOC change for 15% of the 1250 agriculturally designated soil landscape of Canada (SLC) polygons. Simulations were carried out for two to five crop rotations and for conventional and no-tillage. The results indicate that the agricultural soils in Canada, whose SOC are currently very close to equilibrium, will stop being a net source of CO2 and will become a sink by the year 2000. Rates of carbon change for the years 1970, 1990, and 2010 were estimated to be −67, − 39, and 11 kgC ha−1. The rate of decline in the carbon content of agricultural soils in Canada has slowed considerably in the 1990s as a result of an increase in the adoption of no-tillage management, a reduction in the use of summer fallowing, and an increase in fertilizer application. We estimate that the proportion of agricultural land storing SOC will have increased from 17% in 1990 to 53% by the year 2000.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford BSL : Blackwell Science Ltd
    Clinical & experimental allergy 29 (1999), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 33 (2003), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 32 (2002), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 34 (2004), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Cat allergy is unique among allergy to mammals in that the major allergen Fel d 1 is a uteroglobin-like protein and not a lipocalin. The biochemical spectrum of the cat allergens is thus uncertain, particularly with regard to the role that a cat lipocalin protein may play in sensitization to cats in allergic individuals.Objective To analyse cDNA encoding a lipocalin allergen and the corresponding recombinant allergen at both the molecular and immunological levels.Methods A submandibular salivary gland cDNA expression library was constructed and screened for clones producing IgE-binding polypeptides. cDNA encoding a lipocalin allergen and its corresponding recombinant allergen were analysed.Results An IgE binding molecule with high sequence identity to the boar salivary lipocalin and the horse lipocalin Equ c 1 allergen was isolated and designated, Fel d 4. Serum from 62.96% of cat-allergic subjects examined had measurable IgE antibody to Fel d 4 but typically at low levels. Despite this in 47% of sera the anti-Fel d 4 IgE titres were higher than the anti-Fel d 1 titres. IgE binding to the lipocalin allergen could be blocked by an allergen extract from cow and to a lesser degree by extracts from horse and dog.Conclusion Fel d 4 is a lipocalin allergen produced by the cat, which binds IgE at relatively high frequency in cat-sensitive individuals. The allergen provides not only a means for investigating differences in the immune response to lipocalin allergens from that found for other mammalian species but also an important reagent for the diagnosis of cat allergy.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 26 (1943), S. 2057-2063 
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Journal of Polymer Science Part A-2: Polymer Physics 8 (1970), S. 207-224 
    ISSN: 0449-2978
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A discussion is given of the behavior of a mathematical model chosen to approximate conditions during precipitation chromatographic fractionation of polymer (Baker Williams fractionation). With only one molecular weight species of polymer a complete solution of the mathematical model was obtained. With more than one species it was necessary to use a computer solution. Curves showing the movement of polymer containing one and two species of polymer are given for a variety of conditions of operation. It was found that the degree of fractionation of polymer approaches a limiting value with increasing length of column. Under some conditions the degree of fractionation may go through a maximum with length of column. The equilibrating distance (distance of travel of the liquid phase required to establish equilibrium of polymer between the liquid and the bed) was found to be very important in determining the fractionation.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 20 (1974), S. 762-770 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The theory of perturbation chromatography and isotopic tracers has been applied to the investigation of the oxygen exchange reaction between carbon monoxide and carbon dioxide in the presence of a copper-zinc oxide catalyst at 205°C and atmospheric pressure. By analyzing the retention times of carbon-14 monoxide and carbon-14 dioxide tracers in six binary carbon monoxide/carbon dioxide carrier gas streams, it has been possible to shed light on the mechanism of the exchange reaction. Two types of carbon monoxide adsorption and two types of carbon dioxide adsorption were identified. Based on a numerical simulation of the experimental data, it has been concluded that the exchange reaction proceeds via the dissociative adsorption of carbon dioxide. Furthermore, the surface oxygen species formed during the dissociative adsorption of carbon dioxide appears to be the active site for the exchange reaction.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 13 (1967), S. 590-593 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A static vapor pressure apparatus is described which is especially suitable for mixtures. A vapor pressure flask with a built-in condenser allows the mixture to be degassed without change in composition. The entire apparatus is assembled from simple parts. Equilibrium is rapidly attained with either an external pressure or a liquid of known vapor pressure used as a reference.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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