ISSN:
1550-7408
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Biologie
Notizen:
Verrucomicrobiales are a newly proposed, deep branched division of bacteria from which only a handful of cultured isolates are available. Culture-independent analysis of environmental samples shows that members of Verrucomicrobiales are widespread and can be divided into several subdivisions. Symbiotic species of Euplotidium (Ciliophora) and Nematodes, epixenosomes and Xiphinemobacter spp. respectively, are according to the 16S sequences also members of this group. Up to now, only minimal sequence data mainly focused on 16S rRNA gene sequences are available for this group of organisms. The phylogenetic relatives of Verrucomicrobiales are still unclear with Chlamydiales and Planctomycetes being the most probable candidates. The present study describes preliminary results on phylogenetic relationships of Verrucomicrobiales by using protein-coding genes like Hsp70 and RpoC/B. The species which are used for this study are Prosthecobacter vanneervenii, P. debontii, Opitutus sp.VeGlc2, O. terrae and epixenosomes. The genes were amplified with designed consensus-degenerated primers and results are available on the Hsp70. The sequences show several differences within the group. Verrucomicrobium and Prosthecobacter sequences cluster together, but in P. debontii, two sequences were found, and the second one appears quite divergent. Also the two Opitutus genes are quite atypical and cluster together in a deep position. Preliminary attempt was performed on Euplotidium-epixenosome complex. However, amplified and cloned sequences revealed to be closely related to ciliate sequences. Further attempt will be performed applying microtiter-plate-subtractive-hybridization to characterize epixenosome genes.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1550-7408.2005.05202003_6_5.x
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