GLORIA

GEOMAR Library Ocean Research Information Access

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1534-4681
    Keywords: Prostate cancer ; Radical prostatectomy ; Recurrent cancer ; Geographical variation ; Follow-up
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Prostate cancer is often diagnosed early enough in its clinical course to permit radical prostatectomy to be done with curative intent, yet many patients experience tumor recurrence. Most patients receive postoperative surveillance, but the intensity of testing varies appreciably. We sought to evaluate the influence of geographic location on the variability of surveillance intensity. Methods: Questionnaires pertaining to postoperative surveillance were mailed to 4467 members of the American Urological Association (AUA). Practice pattern variation was assessed among 24 large metropolitan statistical areas, among nine United States census regions, and by health maintenance organization penetration rate. Results: Of 4467 urologists surveyed, 1416 (32%) responded and 1050 (24%) responses were evaluable. Correlation analysis showed that mean follow-up intensity across modalities surveyed was highly correlated across tumor, node, metastasis (TNM) stages and years postsurgery. We found no significant main effects attributable to metropolitan statistical area, United States (US) census region, or health maintenance organization (HMO) penetration rate for commonly used surveillance modalities: serum prostate-specific antigen (PSA), office visit, and urinalysis. For infrequently used modalities, there were minimal effects on testing intensity of US census region, metropolitan statistical area, and HMO penetration rate. Few two-way and three-way interactions were significant. Conclusions: The utilization of commonly used surveillance modalities by urologists caring for patients after radical prostatectomy is not affected by metropolitan statistical area, US census region, or HMO penetration rate.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Antonie van Leeuwenhoek 57 (1990), S. 191-203 
    ISSN: 1572-9699
    Keywords: antimycin-sensitive respiration ; astaxanthin ; carotenoids ; photoinduced pigmentation ; pigmented yeasts ; Phaffia rhodozyma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Light and antimycin markedly affected growth and carotenoid synthesis by Phaffia rhodozyma. Exposure of the yeast to high light intensities on agar plates resulted in growth inhibition and decreased carotenoid synthesis. The carotenoid compositions of the yeast were also notably changed by light. β-zeacarotene increased, whereas β-carotene and xanthophylls decreased including astaxanthin, phoenicoxanthin, and 3-hydroxy-3′, 4′-didehydro-β,Ψ-caroten-4-one (HDCO). In liquid medium, growth of the wild-type strain (UCD-FST-67-385) was inhibited by antimycin, but this inhibition was relieved by exposure to light. Light also stimulated carotenoid synthesis about twofold in these antimycin-treated cells. Light may have rescued growth by induction of an alternative oxidase system which facilitated electron disposal when the main respiratory chain was inhibited by antimycin. Isolation and characterization of the oxidase enzymes should be useful in strain development for increased carotenoid production.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 33 (1990), S. 705-708 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Heat inactivation ofClostridium botulinum spores was accelerated in atmospheres containing hydrogen gas. Hydrogen gas also moderately increased the thermal destruction ofBacillus spores. Hydrogen gas may react with components inC. botulinum spores such as transition metals producing hydrogen ions or hydrides, which destroy essential spore components. Thermal processing in modified atmospheres may have applications in food processing and in sterilization of medical supplies.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Abdominal imaging 11 (1986), S. 372-374 
    ISSN: 1432-0509
    Keywords: Colon, foreign bodies ; Enema, complications
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A retained enema tube sheath was encountered in 4 outpatients who underwent preparation for barium enema. This object can be detected on a plain abdominal roentgenogram, but is more readily apparent as an intraluminal foreign body during barium enema examination. We suggest that a prominent warning to remove the sheath before taking a cleansing enema be attached to the enema tip.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Contributions to mineralogy and petrology 131 (1998), S. 71-85 
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract A backscattered and secondary electron SEM study of the grain boundary microstructure in quartz mylonites sampled along the length of the retrograde Simplon Fault Zone established three characteristic components. (1) Fine isolated pores (≤ 1 μm diameter) are scattered across two-grain interfaces, preferentially concentrated on surfaces in extension. Pores are uncommon on three-grain junctions and there is no evidence for fluid interconnectivity along three- and four-grain junctions. The fine porosity may develop by accumulation of original, mainly intragranular fluid inclusions to the grain boundary during deformation and recrystallization and by cavitation of grain boundaries during grain boundary sliding. Dynamic cavitation implies that the “ductile” mylonitic deformation is at least locally dilatant and therefore pressure sensitive. (2) Large “vug”-like pores (up to mm-scale) extend along multi-grain boundaries. Observed in all samples, they are most common in the higher initial temperature, coarse-grained samples with a microstructure dominated by grain boundary migration recrystallization. Grains bordering this connected porosity develop perfect crystal faces, undecorated by fine pores or pits. The irregular “lobate” optical microstructure of many migrating grain boundaries actually consists of a series of straight crystal faces. The coarse porosity is probably due to accumulation during dynamic recrystallization of (CO2-rich ?) fluid with a high wetting angle against quartz. (3) In one sample, interconnected sinuous ridges, ≤ 0.2 μm high, are observed to follow three- and four-grain junctions and disjoint into more isolated worms and spheroidal globules. On two-grain interfaces, these are transitional to more branching vein-like or convoluted brain-like forms. The brain-like and globular forms have been observed, with varying frequency, through the range of samples, with the globules attaining sizes of up to 60 μm. Vein structures have also been observed on intragranular fractures. These topologies do not match across adjoining surfaces and must have developed into free space. The ridge-vein-brain-spheroid structure is distinctly different to that previously observed on experimentally healed microcracks and its origin is not unequivocally established. They could represent unstable meniscus necking of a thin grain-boundary phase of low viscosity, developed due to quasi-adiabatic shear and/or local stress-induced dilatancy during microcracking.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1432-072X
    Keywords: Cellulase ; Clostridium thermocellum ; Extracellular enzymes ; Anaerobiosis ; Sulthydryl oxidation ; Metalloenzyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The crude extracellular cellulase from Clostridium thermocellum was oxidatively inactivated by air and inhibited by sulfhydryl reagents. Activity-loss was prevented and reversed by the addition of a high concentration (10 mM) dithiothreitol (DDT) at zero time and up to 24 h respectively. In the presence of a low concentration (0.4 mM) of DTT, the enzyme was more rapidly inactivated than in air alone. This was probably due to autoxidation of the low DTT concentration to H2O2 as shown by its prevention by a high DTT concentration, exclusion of air, or catalase; and by the oxidative inactivation of the enzyme by H2O2. The inactivation by H2O2 could be prevented by a high concentration of DTT but not by air exclusion. EDTA protected the enzyme from inactivation in air by a low concentration of DTT or by H2O2. This is presumably due to the role of metals in oxidation of SH groups. Furthermore, copper (5 μM) also caused inactivation and this was prevented by the presence of a high DTT concentration. Even in the protective atmosphere of a high DTT concentration, cellulase was inactivated by certain apolar chelating agents such as o-phenanthroline and α-α1-dipyridyl, such inactivation being preventable by the prior incubation of the chelator with a mixture of Fe2+ and Fe3+. These data suggest that the clostridial cellulase, unlike the enzyme from aerobic fungi, contains essential sulfhydryl groups and is stimulated by iron. The endo-β-glucanase component of the cellulase complex was not susceptible to oxidative inactivation.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 150 (1988), S. 460-464 
    ISSN: 1432-072X
    Keywords: NAD+-L-glutamate dehydrogenase ; Clostridium botulinum ; Nitrogen metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell-free extracts of proteolytic strains of Clostridium botulinum types A, B and F (group I) were found to have unusually high specific activities of NAD+-dependent L-glutamate dehydrogenase (NAD-GDH). In comparison, nonproteolytic strains of types B, E and F (group II) had low specific activities. The enzyme was purified 131-fold from C. botulinum 113B to a final specific activity of 〉1,092 μmolxmin-1xmg protein-1. The enzyme is a hexamer of a polypeptide of Mr=42,500, and the native molecular weight is 250,800. The apparent K m values for substrates were 5.3 mM for glutamate and 0.028 mM for NAD+ in the deamination reaction, and 7.2 mM for α-ketoglutarate, 243 mM for NH 4 + and 0.028 mM for NADH in the reverse reaction. NADP+ did not serve as a hydrogen acceptor for the enzyme. Activity in the animation direction was inhibited by fumarate, oxalacetate, aspartate, glutamate and glutamine. The results suggest that GDH is important in group I (proteolytic) C. botulinum to generate α-ketoglutarate as a substrate for transamination reactions. We have also found that the high activity decreases significantly when cells are exposed to sodium chloride. Therefore GDH probably has several important physiological roles in group I proteolytic C. botulinum.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 154 (1990), S. 443-447 
    ISSN: 1432-072X
    Keywords: Clostridium botulinum type E ; Tryptophan metabolism ; Nitrogen repression ; Botulinum neurotoxin formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10–20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum type E. On the other hand, the addition of an enzymatic digest of casein (NZ Case) stimulated toxin formation and overcame repression by tryptophan. Immunoblots of proteins in culture fluids using antibodies to type E toxin indicated that tryptophan-repressed cultures produced less neurotoxin protein. Inhibitors of neurotoxin did not accumulate in cultures grown in minimal medium supplemented with high tryptophan. The results suggest that tryptophan availability in foods or in the intestine may be important for toxin formation by C. botulinum type E.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of biomolecular NMR 15 (1999), S. 135-143 
    ISSN: 1573-5001
    Keywords: CH2D relaxation ; order parameters ; protein design ; sidechain dynamics ; ubiquitin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effect of hydrophobic core packing on sidechain dynamics was analyzed by comparing the dynamics of wild-type (WT) ubiquitin to those of a variant which has seven core mutations. This variant, 1D7, was designed to resemble WT by having a well-packed core of similar volume, and we find that its overall level of dynamics is only subtly different from WT. However, the mutations caused a redistribution in the positions of core residues that are dynamic. This correlates with the tendency of these residues to populate unfavorable rotamers, suggesting that strain from poor sidechain conformations may promote increased flexibility as a mechanism to relieve unfavorable steric interactions. The results demonstrate that even when core volume is conserved, different packing arrangements in mutants can alter dynamic behavior.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of bioenergetics and biomembranes 32 (2000), S. 501-506 
    ISSN: 1573-6881
    Keywords: CF1 ; ATP synthase ; proton flow ; electron transport ; slip
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The chloroplast ATP synthase is strictly regulated so that it is very active in the light (rates of ATP synthesis can be higher than 5 μmol/min/mg protein), but virtually inactive in the dark. The subunits of the catalytic portion of the ATP synthase involved in activation, as well as the effects of nucleotides are discussed. The relation of activation to proton flux through the ATP synthase and to changes in the structure of enzyme induced by the proton electrochemical gradient are also presented. It is concluded that the γ and ε subunits of CF1 play key roles in both regulation of activity and proton translocation.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...